United States Patent
US Patent 5891709: Campy-Cefex selective and differential medium for…
US 5891709 · granted 1999-04-06
Abstract
A solid or semi-solid culture medium, designated Campy-Cefex, for the isolation of Campylobacter species. The culture medium includes:(a) a nutrient medium with an energy source effective to support growth of Campylobacter;(b) agar;(c) blood;(d) a first selective agent selected from cycloheximide, its salts, or mixtures thereof; and(e) a second selective agent selected from cefoperazone, its salts, or mires thereof.In use, the sample to be analyzed is inoculated onto the Campy-Cefex culture medium, and subsequently incubated for a sufficient time and under conditions effective to promote growth of Campylobacter. Following incubation, the culture medium may then be examined for the presence of any colonies of Campylobacter.
| Patent Number | 5891709 |
|---|---|
| Title | Campy-Cefex selective and differential medium for campylobacter |
| Filed | 1992-07-13 |
| Granted | 1999-04-06 |
| Inventor(s) | Kwiatek; Kris, Stern; Norman J., Wojton; Boleslaw J. |
| Assignee | The United States of America as represented by the Secretary of the |
| Number of Claims | 11 |
Abstract
A solid or semi-solid culture medium, designated Campy-Cefex, for the isolation of Campylobacter species. The culture medium includes:(a) a nutrient medium with an energy source effective to support growth of Campylobacter;(b) agar;(c) blood;(d) a first selective agent selected from cycloheximide, its salts, or mixtures thereof; and(e) a second selective agent selected from cefoperazone, its salts, or mires thereof.In use, the sample to be analyzed is inoculated onto the Campy-Cefex culture medium, and subsequently incubated for a sufficient time and under conditions effective to promote growth of Campylobacter. Following incubation, the culture medium may then be examined for the presence of any colonies of Campylobacter.
Claim 1
A composition for the isolation of Campylobacter species comprising: (a) a nutrient medium with an energy source effective to support growth of Campylobacter jejuni or Campylobacter coli or both; (b) agar; (c) blood; (d) a first selective agent selected from the group consisting of cycloheximide, salts of cycloheximide and mixtures thereof; and (e) a second selective agent selected from the group consisting of cefoperazone, salts of cefoperazone, and mixtures thereof; and wherein said composition does not include bacitracin, colistin sulfate or novobiocin as selective agents.
Claims
11 totalA composition for the isolation of Campylobacter species comprising: (a) a nutrient medium with an energy source effective to support growth of Campylobacter jejuni or Campylobacter coli or both; (b) agar; (c) blood; (d) a first selective agent selected from the group consisting of cycloheximide, salts of cycloheximide and mixtures thereof; and (e) a second selective agent selected from the group consisting of cefoperazone, salts of cefoperazone, and mixtures thereof; and wherein said composition does not include bacitracin, colistin sulfate or novobiocin as selective agents.