United States Patent
US Patent 6689577: Reagent and procedure for the detection of…
US 6689577 · granted 2004-02-10
| Patent Number | 6689577 |
|---|---|
| Title | Reagent and procedure for the detection of pathogens, especially spirochetes from body fluids |
| Filed | 2000-02-16 |
| Granted | 2004-02-10 |
| Inventor(s) | Bela Pal Bozsik, Janos Albert, Ilona Dufla |
| Assignee | BOZSIK BELA P |
| CPC Classification | C12Q1/04, C12Q1/02, C12Q1/00 |
| Number of Claims | 2 |
Abstract
A reagent for microscopy-based detection of pathogens, especially spirochetes, from body fluids characterized by that containing the following ingredients: tetracain (125-200 mg/l), mannit (1500-2000 mg/l), EGTA (etilene bis[oxyetiline-nitrilo]-tetraacetate), 0.76-0.114 mg/l magnesium-chloride (preferably in amount of 0.10 mg/l), caffeine-sodium-benzoate (2000-4000 mg/l), glucose (1800-2200 mg/l), glycerol (75-105 mg/l), optimally tri-sodium-citrate (preferable in an amount of 10000 mg/l), Hoechst 33342 dye (1.11 mg/l), if required 20 to 40 ml of distilled water and RPMI 1640 culture media to make 100 ml. Also, a method for detecting pathogens.
Claims Summary
1. A reagent for microscopy-based detection of pathogens from body fluids, containing the following ingredients: 125-200 mg/l of tetracaine, 1500-2000 mg/l of mannite, 0.76-0.114 mg/l of etilene bis[exyetiline-nitrilo]-tetraacetate, about 0.60 mg/l of magnesium-chloride, 2000-4000 mg/l of caffeine-sodium-benzoate, 1800-2200 mg/l of glucose, 75-105 mg/l of glycerol, 10000 mg/l of tri-sodium-citrate, 20 to 40 ml of distilled water and a culture media.