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Reduction of ascorbic acid interference in assays of aqueous fluids |
| T978003 |
Reduction of ascorbic acid interference in assays of aqueous fluids
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| Inventor: |
Wu, et al. |
| Date Issued: |
January 2, 1979 |
| Application: |
05/879,573 |
| Filed: |
February 21, 1978 |
| Inventors: |
Wu; Tai-Wing (Rochester, NY)
Yau; Shirley S. (Rochester, NY)
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| U.S. Class: |
435/28 |
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| International Class: |
C12Q 1/26 |
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| Abstract: |
interference caused by ascorbic acid is eliminated by the presence of ascorbate oxidase in reagent compositions used in enzymatic assay of aqueous fluids such as blood serum for a specific analyte wherein the analyte is oxidized to peroxide and the peroxide reacts with an indicator to produce a detectable product. A preferred reagent composition contains an enzyme capable of catalyzing in the presence of oxygen the oxidation of the analyte to a peroxide, an indicator composition to produce a detectable product and ascorbate oxidase. |
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