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Mammalian checkpoint proteins polypeptides and encoding sequences thereof |
| RE40789 |
Mammalian checkpoint proteins polypeptides and encoding sequences thereof
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| Patent Drawings: | |
| Inventor: |
Elledge, et al. |
| Date Issued: |
June 23, 2009 |
| Application: |
10/267,717 |
| Filed: |
October 9, 2002 |
| Inventors: |
Elledge; Stephen J. (Houston, TX)
Sanchez; Yolanda (Cincinnati, OH)
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| Assignee: |
Baylor College of Medicine (Houston, TX) |
| Primary Examiner: |
Sisson; Bradley L |
| Assistant Examiner: |
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| Attorney Or Agent: |
Sickler; Jennifer S.Molen; Monique VanderSewell; Gardere Wynne |
| U.S. Class: |
435/194; 435/193; 530/324; 530/326; 530/350 |
| Field Of Search: |
530/350; 530/326; 530/827; 536/23.5; 435/69.1; 435/252.3; 435/320.1 |
| International Class: |
C12N 9/12; A61K 38/00; C07K 1/00; C12N 9/10 |
| U.S Patent Documents: |
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| Foreign Patent Documents: |
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| Other References: |
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Susan B. Parker, Gregor Eichele, Pumin Zhang, Alan Rawls, Arthur T. Sands, Allan Bradley, Eric N. Olson, J. Wade Harper and Stephen J. Elledge, "P53-Independent Expression of p21.sup.CIP1 in Muscle and Other Terminally Differentiating Cells,"Science, vol. 267, Feb. 17, 1995, pp. 1024-1027. cited by other.
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| Abstract: |
The present invention relates to the isolation of gene sequences encoding mammalian cell cycle checkpoints, as well as the expression of the encoded proteins using recombinant DNA technology. The expressed proteins are used to generate specific antibodies and to inhibit the growth of cells. The human checkpoint gene sequences are used as a probe for a portion of the chromosome associated with tumors and other malignancies, as well as growth and/or development deficiencies. |
| Claim: |
What is claimed is:
1. A purified .Iadd.human .Iaddend.protein.Iadd., wherein the protein is a polypeptide having checkpoint kinase activity and .Iaddend.encoded by .[.the nucleotide sequenceof.]. SEQ ID NO: 3.
2. A purified .Iadd.human .Iaddend.protein .[.comprising the amino acid sequence set forth in.]. .Iadd.having checkpoint kinase activity, wherein the protein comprises .Iaddend.SEQ ID NO: 1.
3. A fusion protein comprising .[.a portion of.]. at least 15 sequential amino acids of .[.the.]. .Iadd.a .Iaddend.carboxy-terminus .[.of the CHK1 protein of claim 2,.]. .Iadd.sequence of SEQ ID NO:1 .Iaddend.and a non-.[.CHk1.]. .Iadd.Chk1.Iaddend.protein .[.sequence.]. .
4. The fusion protein of claim 3, wherein said non-Chk1 protein sequence .[.comprises.]. .Iadd.is combined with .Iaddend.an affinity tag .Iadd.for purification of the fusion protein.Iaddend..
5. The fusion protein of claim 4, wherein said affinity tag .[.comprises.]. .Iadd.is .Iaddend.a histidine tag.
6. A purified .[.Chk1.]. protein .[.encoded by the nucleotide sequence.]. of claim 5.
.Iadd.7. The fusion protein of either claim 3 or claim 6, wherein the non-Chk1 protein is glutathione S-I transferase..Iaddend. |
| Description: |
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