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Compositions and methods of using apoptosis signaling kinase related kinase (ASKRK) |
| RE40624 |
Compositions and methods of using apoptosis signaling kinase related kinase (ASKRK)
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| Patent Drawings: | |
| Inventor: |
Johnson, et al. |
| Date Issued: |
January 20, 2009 |
| Application: |
11/900,008 |
| Filed: |
September 7, 2007 |
| Inventors: |
Johnson; Jeffrey D. (Moraga, CA)
Zhou; Yun-Ping (East Brunswick, NJ)
Marlen; Kimberly (San Pablo, CA)
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| Assignee: |
Metabolex, Inc. (Hayward, CA) |
| Primary Examiner: |
Mondesi; Robert B. |
| Assistant Examiner: |
Gebreyesus; Kagnew H |
| Attorney Or Agent: |
Townsend and Townsend and Crew LLP |
| U.S. Class: |
435/252.3; 435/194; 435/320.1; 435/325; 435/69.1; 536/23.2 |
| Field Of Search: |
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| International Class: |
C12N 15/56 |
| U.S Patent Documents: |
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| Foreign Patent Documents: |
WO 01/77338; WO 02/46384; WO 03/064639 |
| Other References: |
Butler, et al., "Beta-Cell Deficit and Increased Beta-Cell Apoptosis in Humans with Type 2 Diabetes." Jan. 2003, Diabetes, vol. 52, No. 1,102-110. cited by other.
Gotoh et al., "Reactive Oxygen Species- and Dimerization-induced Activation of Apoptosis Signal-regulating Kinase 1 in Tumor Necrosis Factor-.alpha. Signal Transduction", J Biol Chem, 1998, pp. 17477-17482, vol. 273. cited by other.
Hatai et al., "Execution of Apoptosis Signal-regulating Kinase 1 (ASK1)-induced Apoptosis by the Mitochondria-dependent Caspase Activation", J Biol Chem, 2000, pp. 26576-26581, vol. 275. cited by other.
Ichijo, et al., Induction of Apoptosis byASK1, a Mammalian MAPKKK That Activates SAPK/JNK and p38 Signaling Pathways, Science, 1997, pp. 90-94, vol. 275. cited by other.
Johnson et al., "Mitogen-Activated Protein Kinase Pathways Mediated by ERK, JNK, and p38 Protein Kinases", Science, 2002, pp. 1911-1912, vol. 298. cited by other.
Kagi et al., "TNF Receptor 1-Dependent .beta. Cell Toxicity as an Effector Pathway in Autoimmune Diabetes", J Immunol, 1999, pp. 4598-4605, vol. 162. cited by other.
Kaneto et al., "Apoptotic Cell Death Triggered by Nitric Oxide in Pancretic .beta.-Cells", Diabetes, 1995, pp. 733-738, vol. 44. cited by other.
Kyriakis et al., "Mammalian Mitogen-Activated Protein Kinase Signal Transduction Pathhways Activated by Stress and Inflammation", Physiol Rev, 2001, pp. 807-869, vol. 81. cited by other.
Liu et al., "Thioredoxin Promotes ASK1 Ubiquitination and Degradation to Inhibit ASK1-Mediated Apoptosis in a Redox Activity-Independent Manner", Circ Res, 2002, pp. 1259-1266, vol. 90. cited by other.
Mandrup-Poulsen, et al., "Human Tumor Necrosis Factor Potentiates Human Interleukin 1-Mediated Rat Pancreatic .beta.-Cell Cytotoxicity", J Immunol, 1987, pp. 4077-4082, vol. 139. cited by other.
Mathis et al., ".beta.-Cell death during progression to diabetes", Nature, 2001, pp. 792-798, vol. 414. cited by other.
Nishitoh et al., "ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats", Genes and Development, 2002, pp. 1345-1355, vol. 16. cited by other.
Takeda et al., "Roles of MAPKKK ASK1 in Stress-Induced Cell Death", Cell Structure and Function, 2003, pp. 23-29, vol. 28. cited by other.
Tibbles et al., "The stress-activated protein kinase pathways", Cell Mol. Life Sci., 1999, pp. 1230-1254, vol. 55. cited by other.
Tobiume et al., "ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis", EMBO Reports, 2001, pp. 222-228, vol. 2. cited by other.
Tobiume et al., "Activation of Apoptosis Signal-Regulating Kinase 1 by the Stress-Induced Activating Phosphorylation of Pre-Formed Oligomer", J Cell Physiology, 2002, pp. 95-104, vol. 191. cited by other.
Tournier et al., "Requirement of JNK for Stress-Induced Activation of the Cytochrome c-Mediated Death Pathway", Science 2000, pp. 870-874, vol. 288. cited by other.
Zhou et al., "Apoptosis in Insulin-secreting Cells", J Clin. Invest., 1998, pp. 1623-1632, vol. 101. cited by other.
Database EMBL [Online] , Feb. 22, 2002 (Feb. 22, 2002), "ih13f08.y1 Human insulinoma Homo sapiens cDNA clone Image: 5' similar to SW:M3K4_Mouse 035099 Mitogen-Activated Protein Kinase 5;, mRNA sequence." XP002441451, retrieved from EBI Accession No.EMBL: BM508924, Database Accession No. BM508924. cited by other.
Database EMBL [Online] Jan. 24, 2003 (Jan. 24, 2003), "Mus musculus mitogen-activated protein kinase kinase kinase 15, mRNA (cDNA clone Image: 4975522), containing frame-shift errors." XP00244152 retrieved from EBI Accession No. EBL: BC043943,Database Accession No. BC043943. cited by other.
Mathis et al., ".beta.Cell death during progression to diabetes", Nature, 2001, pp. 792-798, vol. 434. cited by other. |
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| Abstract: |
The invention provides apoptosis signaling kinase related kinase (ASKRK) nucleic acid and polypeptide sequences and methods of using such sequences to identify modulators of ASKRK. Such modulators can be used for the treatment of diabetes or for delaying the onset of diabetes. The invention also provides methods of diagnosing diabetes or pre-diabetes and methods of making a prognosis based on the detection of ASKRK nucleic acids and proteins. |
| Claim: |
What is claimed is:
1. An isolated nucleic acid encoding a polypeptide comprising SEQ ID NO:2.
2. The isolated nucleic acid of claim 1, wherein the nucleic acid comprises SEQ ID NO:1.
3. An expression vector comprising the nucleic acid of claim 1.
4. A host cell in vitro comprising the expression vector of claim 3.
5. The host cell of claim 4, wherein the host cell is a pancreatic beta cell. |
| Description: |
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