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Geranium plant "Siren" |
| PP6379 |
Geranium plant "Siren"
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| Patent Drawings: | |
| Inventor: |
Craig |
| Date Issued: |
November 8, 1988 |
| Application: |
06/882,069 |
| Filed: |
July 3, 1986 |
| Inventors: |
Craig; Richard (State College, PA)
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| Assignee: |
Research Corporation (New York, NY) |
| Primary Examiner: |
Bagwill; Robert E. |
| Assistant Examiner: |
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| Attorney Or Agent: |
Scully, Scott Murphy & Presser |
| U.S. Class: |
PLT/330 |
| Field Of Search: |
Plt/68 |
| International Class: |
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| U.S Patent Documents: |
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| Foreign Patent Documents: |
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| Other References: |
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| Abstract: |
This invention relates to a new distinct cultivar of geranium substantially as illustrated and described characterized as being particularly well adapted to both commercial greenhouse production and garden performance, and possessing unique flavonol and anthocyanin profiles and double flowers when compared to geranium cultivar "Bruni". |
| Claim: |
What is claimed is:
1. A new distinct cultivar of geranium substantially as illustrated and described characterized as being particularly well adapted to both commercial greenhouse production andgarden performance, and possessing unique flavonol and anthocyanin profiles and double flowers when compared to the geranium cultivar "Bruni". |
| Description: |
The present invention relates to a new anddistinct cultivar of geranium Pelargonium.times.hortorum called "Siren". The cultivar is particularly well adapted to both commercial greenhouse production as well as garden performance. The cultivar is characterized as to novelty as having doubleflowers when compared to the parent geranium cultivar "Bruni". The cultivar is further characterized by unique biochemical fingerprint profiles.
The cultivar was developed from an organized, scientifically designed breeding program carried out at the Department of Horticulture, The Pennsylvania State University, University Park, PA 16802 and specifically was selected from theself-pollinated progeny of the geranium cultivar "Bruni". The selection was asexually propagated by cuttings and the reproductions ran true.
With reference to the detailed description of the cultivar which follows, the test plant was grown in full sun under glass, 62.degree. F. night and 68.degree. F. cloudy days, 75.degree. F. sunny days. Soilless medium was fertilized once perweek with 15-15-15 at 275 ppm N. Color readings were taken under cool white fluorescent lamps at 150 footcandles and color identification was by reference to The Royal Horticultural Society Colour Charts except where common terms of color definition areemployed.
DESCRIPTION OF THE FIGURES
FIG. 1 illustrates in color the cultivar including foliage and flowers.
FIG. 2 illustrates the anthocyanin profile obtained from HPLC. Quantitative values are found in the tables. Analyses included a single peak that represented both pelargonidin and petunidin 3,5-diglucosides. Corrections were made inaccompanying tables.
Peak No.
1. Delphinidin 3,5-diglucoside
2. Cyanidin 3,5-diglucoside
3. Pelargonidin 3,5-diglucoside
4. Peonidin 3,5-diglucoside
5. Malvidin 3,5-diglucoside
FIG. 3 illustrates the flavonol profile obtained from HPLC. Quantitative values are found in the tables.
Peak No.
1. Quercetin 3-rhamnosylgalactoside
2. Quercetin 3-rutinoside
3. Quercetin 3-galactoside
4. Quercetin 3-glucoside
5. Kaempferol 3-rhamnosylgalactoside
6. Kaempferol 3-galactoside
7. Kaempferol 3-rutinoside
8. Kaempferol 3-glucoside; Kaempferol 7-glucoside; Quercetin 3-rhamnoside
9. Kaempferol 3-xyloside
10. Kaempferol 3-arabinoside
11. Kaempferol 3-rhamnoside
THE PLANT
Classification:
Botanical.--Pelargonium.times.hortorum.
Tradename.--#719 (80-210-27)="Siren".
Form: Compact, globe, symmetrical.
Height: 25.0-30.0 cm.
Growth: Short internodes, self branching; leaves and stem somewhat small; flowers borne close to foliage.
Strength: Excellent, uniform.
Leaves:
Size.--6.0-11.0 cm.
Shape.--Reniform, variously lobed.
Margin.--Crenate.
Texture.--Pubescent.
Color.--Top: Fan 3, green group 137-B (RHSCC). Bottom: Fan 3, green group 138-B (RHSCC).
Ribs and veins.--Palmate veins; Ribs suppressed and distinct, Fan 3, green group 143-C (RHSCC).
Petioles: Stalked 6.0-9.0 cm.
Stem:
Color.--Fan 3, yellow-green group 144-A (RHSCC).
Internodes.--1.5-2.0 cm.
THE BUD
Shape: Globular.
Size: 1.5 cm diameter.
INFLORESCENCE
Blooming habit: Medium to small diameter bloom, very double-looking, ball-shaped head, flowers close to foliage.
Size: 7.0-10.0 cm. across.
Borne: Heavy, strong peduncle; modified umbel.
Florets:
Form.--Round-cupped, 3.5-5.0 cm -- has double appearance from twisted, irregular petals.
Petals.--12-16.
Size.--1.5-2.0 cm long and 1.0-1.5 cm wide.
Texture and appearance.--Smooth-satiny, dark cherry red. Top: Fan 1, red group -- 43-A (RHSCC). Bottom: Fan 1, red group -- 40-B (RHSCC).
Petaloids:
Quantity.--2-4.
Shape.--Twisted.
Color.--Same as petals.
Pedicel: 2.0-2.5 cm in length.
Peduncle: 12.0-14.0 cm long, heavy and strong.
Persistence: Non-shattering.
Disease resistance: Not evaluated.
REPRODUCTIVE ORGANS
Stamens:
Anthers.--Small, purplish 2.0-3.0 mm.
Filaments.--Flat white, 5.0-7.5 mm long, top red.
Pollen.--Yellow.
Pistils:
Number.--1.
Length.--1.0 cm.
Stigma.--4- or 5-lobed, orange.
Style.--Green; 5 cm.
Ovaries: Green, pubescent; 4- or 5-lobed.
Fruit: None.
BIOCHEMICAL PROFILES
In recent years, biochemical analysis has played an increasing role in plant systematics and taxonomy. In order to further characterize the cultivar, flavonols and anthocyanins were extracted from florets of the cultivar and its parent andsubjected to analysis by high pressure liquid chromatography (HPLC). Background information supporting the validity of the HPLC technique can be found in an article by Asen & Griesbach ("High Pressure Liquid Chromatographic Analysis of Flavonoids inGeranium Florets as an Adjunct for Cultivar Identification", S. Asen and R. Griesbach, J. Amer. Soc. Hort. Sci. 108(5):845-850 (1983)), the contents of which are incorporated herein by reference. Briefly, the method for performing the analysis wascarried out as follows:
Flavonoid extraction.
The sample size for flavonoid identification consisted of the petals from six florets just after anthesis. Three different samples were collected from each cultivar and handled separately for analysis. The petals were weighed, ground in 20 mlof 1% HCl-MeOH with a mortar and pestle, filtered through one layer of Whatman #1 filter paper, and washed with 1% HCl-MeOH. The volume was adjusted to 90 ml and 2-15 ml aliquots were removed for the analysis and handled separately. Each aliquot wastaken to dryness at 40.degree. C. in vacuo. All traces of HCl were removed by azeotropic distillation with MeOH. One of the dried extracts was reconstituted in 2 ml of 1% HCl-MeOH and was used for anthocyanin analysis. The other was reconstituted in2 ml of MeOH and was used for flavonol analysis. Each sample was stored at -34.degree. C. until analyzed.
HPLC.
Samples were analyzed on a Waters High Performance Liquid Chromatograph equipped with an automatic injection system (Waters Assoc. Wisp 71OA), dual pumps (Waters Assoc. Model 6000A), solvent programmer (Waters Assoc. Model 600), data module(Waters Assoc.), variable wavelength detector (Waters Assoc. Model 480), and a C.sub.18 column (25 cm.times.0.46 cm and 5 .mu.m particle size, Supelco).
Most of the flavonol compounds were separated by a linear gradient of 8% to 23% pump B over 55 min (pump A=1% triethylamine buffered to pH 3.0 with H.sub.3 PO.sub.4 (TEAP); pump B=CH.sub.3 CN) at a flow rate of 1.2 ml/min and a chart speed of 0.5cm/min. Detection was at 340 nm.
The anthocyanins were resolved by a linear gradient of 30% to 50% pump B over 40 min (pump A=1.5% H.sub.3 PO.sub.4 ; pump B=20% HOAc+25% CH.sub.3 CN+55% of 1.5% H.sub.3 PO.sub.4) at a flow rate of 1.0 ml/min and a chart speed of 0.5 cm/min.Detection was at 546 nm utilizing a fixed wavelength detector.
The flavonoids were quantified by injecting standards and comparing their peak areas with those from the plant samples. The results are expressed as .mu.g of flavonoid/g fresh weight of plant material.
Results
Chromatographic profiles for anthocyanins and flavonols are presented in FIGS. 2 and 3, respectively; quantification of these profiles by comparison to standards is presented in Tables 1 and 2, respectively.
The anthocyanins petunidin and pelargonidin 3,5-diglucoside were not resolved by the solvent system used. Past research has shown only negligible amounts of petunidin 3,5-diglucoside to be present in geranium florets compared to pelargonidin3,5-diglucoside. In light of this, the peak corresponding to petunidin and pelargonidin 3,5-diglucoside was quantified as pelargonidin 3,5-diglucoside.
Kaempferol 3-rhamnoside could not be quantitated for several cultivars and is designated as ND (not determined). The chromatograms showed a small, wide peak in the region of elution for this compound. If a substantial amount of this compoundwere present, a distinct peak appeared but minute quantities, if present, were masked.
Other barriers to quantitation of several flavonols existed. Kaempferol 3-glucoside, kaempferol 7-glucoside, and quercetin 3-rhamnoside all had the same retention time under these conditions. If these compounds are needed to distinguish betweencultivars, they would have to be separated by other solvents or column types. Quercetin 3-xyloside appeared in several of the comparisons, but standards were not available to quantify this compound.
TABLE 1 ______________________________________ Anthocyanin concentration in petals of geranium florets .mu.g anthocyanidin 3,5-diglucoside/g fresh wt. Cul- Delphin- Cya- Pelar- tivar idin nidin gonidin Peonidin Malvidin Total ______________________________________ 719 --.sup.z 149 1933 1461 43 3586 Bruni -- 78 6251 2418 -- 7747 ______________________________________ .sup.z -- = not detected.
TABLE 2 ______________________________________ Flavonol concentration in petals of geranium florets ______________________________________ .mu.g/g fresh wt. Qu3-.sup.z Qu3- Qu3- Qu3- Km3- Cultivar rhagal rut gal glu rhagal ______________________________________ 719 t.sup.y t t 18 78 Bruni 11 34 --.sup.w t 307 ______________________________________ .mu.g/g fresh wt. Km3- Km3- Km3- Km3- Km3- Cultivar gal rut xyl arab rha Total ______________________________________719 178 171 34 117 ND.sup.x 611 Bruni 47 1257 13 49 185 1908 ______________________________________ .sup.z Abbreviations: Km = Kaempferol; Qu = Quercetin; arab = arabinoside; gal = galactoside; glu = glucoside; rha = rhamnoside rhagal =rhamnosylgalactoside; rut = rutinoside; xyl = xyloside. .sup.y t = trace <10 .mu.g. .sup.x ND = not determined. .sup.w -- = not detected.
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