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Circovirus sequences associated with piglet weight loss disease (PWD)
7622126 Circovirus sequences associated with piglet weight loss disease (PWD)
Patent Drawings:Drawing: 7622126-10    Drawing: 7622126-11    Drawing: 7622126-12    Drawing: 7622126-13    Drawing: 7622126-14    Drawing: 7622126-15    Drawing: 7622126-16    Drawing: 7622126-17    Drawing: 7622126-18    Drawing: 7622126-19    
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Inventor: Jestin, et al.
Date Issued: November 24, 2009
Application: 11/588,237
Filed: October 27, 2006
Inventors: Jestin; Andre (Saint-Brieuc, FR)
Albina; Emmanuel (Tregueux, FR)
Le Cann; Pierre (Pledran, FR)
Blanchard; Philippe (Plerin, FR)
Hutet; Evelyne (Plerin, FR)
Arnauld; Claire (Saint-Brieuc, FR)
Truong; Catherine (Saint-Brieuc, FR)
Mahe; Dominique (Saint-Carreuc, FR)
Cariolet; Roland (Ploufragan, FR)
Madec; Francois (Saint-Brieuc, FR)
Assignee: Wyeth (Madison, NJ)
Primary Examiner: Salimi; Ali R.
Assistant Examiner:
Attorney Or Agent: Bingham McCutchen LLP
U.S. Class: 424/204.1
Field Of Search: 424/204.1
International Class: A61K 39/12
U.S Patent Documents:
Foreign Patent Documents: 10/044648; 0 737 750; 2 422 956; 2518 755; 2769322; 2772047; 1 538 305; WO 90/11092; WO 92/05255; WO 94/01133; WO 94/21797; WO 94/27238; WO 94/27435; WO 95/11307; WO 96/34109; WO 96/40931; WO 96/40945; WO 98/40499; WO 99/18214; WO 99/29717; WO 99/29871; WO 00/01409; WO 00/24428; WO 00/77188; WO 00/77216; WO 01/96377; WO 02/102999
Other References: ABI-Prism, "Automated DNA Sequencing, Chemistry Guide", Applied Biosystems, product manual, applicable to automated sequencers ABI Prism 310,377 and 373 (2000). cited by other.
Adams, Mark D. et al. "Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project," Science, 1651-1656. (1991). cited by other.
Albina et al., "Premiers resultats du CNEVA sur le deperissement fatal du porcelet en fin de post-sevrage" La Semaine Veterinaire des Filieres, 26:1-2, Nov. 30, 1996. cited by other.
Allan et al., "Porcine Circoviruses: A Review," J Vet Diagn Invest. 12: 3-14 (2000). cited by other.
Allan et al., "Immunostimulation, PCV-2 and PMWS," Veterinary Record (2000); 147(6):170-171. cited by other.
Allan, G.M., et al., "Production, preliminary characterisation and applications of monoclonal antibodies to porcine circovirus," Veterinary Immunology and Immunopathology, 43 (1994) 357-371. cited by other.
Altmann, Curtis R. et al. "Microarray-Based Analysis of Early Development in Xenopus laevis," Developmental Biology, 236:64-75 (2001). cited by other.
Bantle, John A. et al. "Phase III Interlaboratory Study of FETAX Part 3. FETAX Validation using 12 Compounds with and without an Exogenous Metabolic Activation System," Journal of Applied Toxicology, 19:447-472 (1999). cited by other.
Behr, J.P., "Gene Transfer with Synthetic Cationic Amphiphiles: Prospects for Gene Therapy" Bioconjugate Chem. 5(5):382-389 (1994). cited by other.
Bei, R. et al., "The Use of a Cationic Liposome Formulation (DOTAP) Mixed with a Recombinant Tumor-Associated Antigen to Induce Immune Responses and Protective Immunity in Mice," Journal of Immunotherapy, 21(3):159-169 (1998). cited by other.
Cho et al., "Enhanced cellular immunity of hepatitis C virus nonstructural proteins by codelivery of granulocyte macrophage-colony stimulating factor gene in intramuscular DNA immunization," Vaccine 17:1136-1144 (1999). cited by other.
Cruse et al., "The Illustrated Dictionary of Immunology," Boca Raton: CRC Press (1995) p. 156. cited by other.
Cruse et al., "The Illustrated Dictionary of Immunology," 2nd edition. Boca Raton: CRC Press (2003) p. 613. cited by other.
Dedet, V., "Seule certitude: un maladie a part entiere," La Semaine Veterinaire p. 54, May 24, 1997. cited by other.
DMRIE structure, Printed from PubChem Compund Summary 2008. cited by other.
Donnelly, John J. et al., "Immunization with DNA" Journal of Immunological Methods, 176:145-152 (1994). cited by other.
Dorland's Illustrated Medical Dictionary, 28th edition. Philadelphia. WB Saunders p. 1787 (1994). cited by other.
Ellis et al., "Reproduction of lesions of Post eaning multisystemic wasting syndrome in gnotobiotic piglets," J. Vet. Diagn. Invest. 11:3-14 (1999). cited by other.
Ellis et al., "Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome" J. Vet. Diagn. Invest. 12(1):21-27 (2000). cited by other.
Ertl, H.C.J. et al., "Genetic Immunization" Viral Immunology, 9(1):1-9 (1996). cited by other.
Felgner J.H. et al., "Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations," The journal of biological chemistry, 269(4)2550-2561 (1994). cited by other.
Fenaux, M. et al., "Genetic Characterization of Type 2 Porcine Circovirus (PCV-2) from Pigs with Postweaning Multisystemic Wasting Syndrome in Different Geographic Regions of North America and Development of a Differential PCR-Restriction FragmentLength Polymorphism Assay to Detect and Differentiate between Infections with PCV-1 and PCV-2," Journal of Clinical Microbiology, Jul. 2000, 38(7):2494-2503. cited by other.
Fort, Douglas J. et al. "Evaluation of the Developmental Toxicity of Thalidomide Using Frog Embryo Teratogenesis Assay--Xenopus (FETAX): Biotransformation and Detoxification," Teratogensis, Carcinogenesis, and Mutagenesis, 20:35-47 (2000). cited byother.
GenBank Accession No. AF027217; May 14, 1998. cited by other.
Gregoriadis G. et al., "Liposome-mediated DNA vaccination" FEBS Letters, Feb. 3, 1997; 402:107-110. cited by other.
Gregoriadis, Gregory, "Genetic vaccines: strategies for optimization" Pharmaceutical Research, 15( 5):661-670 (1998). cited by other.
Grierson, S.S., et al., "Genome sequence analysis of 10 Dutch porcine circovirus type 2 (PCV-2) isolates from a PMWS case-control study," Research in Veterinary Science, 77 (2004) pp. 265-268. cited by other.
Haddad, D. et al., "Comparative study of DNA-based immunization vectors: effect of secretion signals on the antibody responses in mice," FEMS Immunology and Medical Microbiology, 18(3):193-202 (1997). cited by other.
Hamel et al., "Porcine Circovirus," (Sequence Alignments for SEQ ID NO:s 1-4 and 6) Genbank Acc No. 027217, Dec. 19, 1997. cited by other.
Inumaru, S. et al., "cDNA Cloning of Porcine Granulocyte-Macrophage Colony-Stimulating Factor" Immunology and Cell Biology, 73:474-476, XP-000946635 (1995). cited by other.
Ishii N. et al., "Cationic liposomes are a strong adjuvant for a DNA vaccine of human immunodeficiency virus type 1" AIDS Res Hum Retroviruses, Nov. 1, 1997 13(6):1421-1428. cited by other.
Izumida, et al., "Establishment of the Attenuated Strain of Porcine Parvovirus of the Live Vaccine and its Biological-Immunological Characteristics," Japanese Veterinary Science, 48(2):293-303 (1996). cited by other.
Klavinskis, Linda S. et al., "Intranasal Immunization with Plasmid DNA-Lipid Complexes Elicits Mucosal Immunity in the Female Genital and Rectal Tracts" J. Immunol 1999, pp. 254-262. cited by other.
Knell, S. et al., "Comparative genetic characterization of Porcine Circovirus type 2 samples from German wild boar populations," Veterinary Microbiology, 109 (2005) pp. 169-177. cited by other.
Krakowka et al., "Activation of the Immune System in the Pivotal Event in the Production of Wasting Disease in Pigs Infected with Porcine Circovirus-2 (PCV-2)," Vet Pathology. 38:31-42 (2001). cited by other.
Krakowka et al., "Immunologic Features of Porcine Circovirus Type-2 Infection," Viral Immunology. 15(4): 567-582 (2002). cited by other.
LeCann et al., "Piglet Wasting disease," Veterinary Record, p. 660, Dec. 20/27, 1997. cited by other.
Lekcharoensuk, P. et al., "Epitope Mapping of the Major Capsid Protein of Type 2 Porcine Circovirus (PCV2) by Using Chimeric PCV1 and PCV2," Journal of Virology, vol. 78, No. 15, Aug. 2004, pp. 8135-8145. cited by other.
Mahe et al., "Differential Recognition of ORF 2 Protein from Type 1 and Type 2 Porcine Circoviruses and Identification of Immunorelevant Epitopes", Journal of General Virology 81:1815-1824 (2000). cited by other.
Mankertz et al., "Porcine Circovirus Complete Genome", EMBL Sequence Database XP-002104869 May 22, 1997. cited by other.
McCluskie, Michael J. et al., "Route and Method of Delivery of DNA Vaccine Influence Immune Responses in Mice and Non-Human Primates" Molecular Medicine 5:287-300 (1999). cited by other.
McInnes, C.J. et al., "Cloning and Expression of a cDNA Encoding Ovine Granulocyte-Macrophage Colony-Stimulating Factor" Gene, 105: 275-279, XP-002148815 (1991). cited by other.
Meehan et al., "Putative PCV Replication-Associated Protein (REP)", EMBL Sequence Database XP 002104867 (1997). cited by other.
Meehan et al., "Porcine Circovirus Complete Genome", EMBL Sequence Database XP-002104868 (1997). cited by other.
Meehan, B.M. et al., "Characterization of noval circovirus DNAs associated with wasting syndromes in pigs.", Journal of General Virology, vol. 79, No. 9, pp. 2171-2179, Sep. 1998, XP-002090386. cited by other.
Miller, J.S. et al., "The nucleotide sequence of RNA-1 of Indian peanut clump furovirus" Arch. Virol. 141:2301-2312, (1996). cited by other.
Mumford J. A. et al. "Antigenicity and immunogenicity of equine infleuenza vaccines containing a Carbomer adjuvant," Epidemiol. Infect. 112: 421-437 (1994). cited by other.
Nakakura, Norihiko et al. "Synthesis of Heterogeneous mRNA-like RNA and Low-Molecular-Weight RNA before the Midblastula Transition in Embryos of Xenopus laevis," Developmental Biology, 123:421-429 (1987). cited by other.
Nash RA et al., "Molecular cloning and in vivo evaluation of canine granulocyte-macrophage colony-stimulating factor" Blood 78(4):930-937, XP 002133949 (1991). cited by other.
Nawagtigul et al., "Open Reading Frame 2 of Porcine Circovirus Type 2 Encodes a Major Capsid Protein", Journal of General Virology 81:2281-2287 (2000). cited by other.
Nayer et al., "Detection and Characterization of Porcine Circovirus Associated With Postweaving Multisystemic Wasting Syndrome Pigs", Can Vet. J. 38:385-386 (1997). cited by other.
Newport, John et al. "Major Developmental Transition in Early Xenopus Embryos: I. Characterization and Timing of Cellular Changes at the Midblastula Stage," Cell 30:675-686 (1982). cited by other.
Newport, John et al. "A Major Developmental Transition in Early Xenopus Embryos: II. Control of the Onset of Transcription," Cell 30:687-696 (1982). cited by other.
Nieuwkoop and Faber "Normal Table of Xenopus laevis (Daudin)--A Systematic and chronological survey of the development from the fertilized egg to the end of metamorposis," Chapter VII, 162-188 Garland Publishing(1994). cited by other.
Norman, JA et al., "Development of Improved Vectors for DNA-Based Immunization and other Gene Therapy Applications," Vaccine, 15(8): 801-803 (1997). cited by other.
Nuwaysir, Emile F. et al. "Microarrays and Toxicology: The Advent of Toxicogenomics," Molecular Carcinogenesis, 24:153-159 (1999). cited by other.
Okada, E. et al., "Intranasal immunization of a DNA vaccine with IL-12- and Granulocyte-macrophage colony-stimulating Factor (GM-(SF))--expressing plasmids in Liposomes Induces Strong Mucosal and Cell-Mediated immune responses against HIV-1antigens," The Journal of Immunology, 159:3638-3647 (1997). cited by other.
Parker, SE et al., "Plasmid DNA gene therapy: Studies with the human interleukin-2 gene in tumor cells in vitro and in the murine B16 melanoma model in vivo," Cancer Gene Therapy, 3(3):175-185 (1996). cited by other.
Restifo, N P et al. "The promises of nucleic acid vaccines,", Gene Therapy, 7:89-92 (2000). cited by other.
Ruitenberg, K. M. et al., "DNA-Mediated Immunization with Glycoprotein D of Equine Herpesvirus 1 (EHV-1) in a Murine Model of EHV-1 Respiratory Infection," Vaccine, 17:237-244 (1999). cited by other.
Schena, Mark, et al. "Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray," Science, 270:467-470 (1995). cited by other.
Schena, Mark et al. "Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes," Proc. Natl. Acad. Sci. USA, 93:10614-10619 (1996). cited by other.
Schultz, J et al., "Update on Antiviral DNA Vaccine Research (1998-2000)," lntervirology, 43:197-217 (2000). cited by other.
Sin, J-I et al., "Protective Immunity Against Heterologous Challenge with Encephalomyocarditis Virus by VP1 DNA Vaccination: Effect of Coinjection with a Granulocyte-Macrophage Colony Stimulating Factor Gene," Vaccine 15:1827-1833 (1997). cited byother.
Somasundaram, C. et al., "Enhanced Protective Response and Immuno-Adjuvant Effects of Porcine GM-CSF on DNA Vaccination of Pigs against Aujeszky's Disease Virus," Veterinary Immunology and Immunopathology 70:277-287 (1999). cited by other.
Terpestra et al., "Potentcy control of modified live viral vaccines for veterinary use," Vaccine 14(6):570-575 (1996). cited by other.
Todd et al., "Comparision of Three Animal Viruses with Circular Single-Stranded DNA Genomes," Arch Virol., 117:129-135 (1991). cited by other.
Vannier, et al., "Study of the Efficacy of an Inactivated Virus Vaccine Against Porcine Parvovirus," Ann. Rech. Vet. 17(4):425-432 (1986). cited by other.
Vogel, et al., "Nucleic Acid Vaccines," Clinical Microbiology Reviews 8(3):406-410 (1995). cited by other.
Walker, G.T. et al., Nucleic Acids Res. (1992) 20: 1691-1696. cited by other.
Watanabe, Y et al., "Highly Efficient Transfection into Primary Cultured Mouse Hepatocytes by Use of Cation-Liposomes: An Application for Immunization," J. Biochem. 116:1220-1226 (1994). cited by other.
Wen, L. et al., "Genotyping of porcine circovirus type 2 from a variety of clinical conditions in China," Veterinary Microbiology, 110 (2005) pp. 141-146. cited by other.
West et al., "Myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2" J Vet Diagn Invest. 11: 530-532 (1999). cited by other.
Wheeler, C.J. et al., "Converting an alcohol to an amine in a cationic lipid dramatically alters the co-lipid requirement, cellular transfection activity and the ultrastructure of DNA-cytofectin complexes," Biochemica et Biophysica Acta 1280:1-11,XP 002035803 (1996). cited by other.
Xiang Z. et al., "Manipulation of the immune response to a plasmid-encoded viral antigen by coinoculation with plasmids expressing cytokines," Immunity, 2:129-135 (1995). cited by other.
Yokoyama et al. "DNA immunization: Effects of vehicle and route of administration on the induction of protective antiviral immunity," FEMS Immunology and Medical Microbiology, 14:221-230 (1996). cited by other.
Young, "Fields Virology" 3rd ed. Philadelphia: Lippencott-Raven Publishers Chapter 70- Parvoviruses; vol. 2, p. 2199-2220 (1996). cited by other.
Zhang, Michael Q. "Large-Scale Gene Expression Data Analysis: A New Challenge to Computational Biologists," Genome Research, 9(8):681-688 (1999). cited by other.
Nawagitgul et al., Modified Indirect Porcine Circovirus (PCV) Type 2-Based and Recombiant Capsid Protein (CRF2)-Based Enzyme-Linked immunosorbent Assays for Detection of Antibodies to PCV, Clinical and Diagnostic Laboratory Immunology, Jan. 2002,vol. 9, No. 1, pp. 33-40, see the abstract. cited by other.
Walker IW, et al., (J Vet Diagn Invest. 2000; 12:400-5). cited by other.
Liu Q, et al., (Protein Expr Purif. 2001; 21:115-20). cited by other.
Kim, Yuna et al., Characterization of the Recombinant Proteins of Porcine Circovirus Type 2 Field Isolate Expressed in the Baulovirus System, J. Vet. Scif. (2002), 3(1), 19-23. cited by other.
European Search Report dated Oct. 16, 2008. cited by other.
Allan, G. M. et al., "Isolation of Procine Circovirus-like Viruses from Pigs with a Wasting Disease in the USA and Europe", Journal of Veterinary Diagnostic Investigation, vol. 10, pp. 3-10, Jan. 1998, XP 002068503. cited by other.
Allan, G.M. et al., 1995, Vet. Microbiol., 44: 49-64. cited by other.
U.S. Appl. No. 60/069,750, filed Dec. 16, 1997, Wang et al. cited by other.
U.S. Appl. No. 60/069,233, filed Dec. 11, 1997, Wang et al. cited by other.
Author Unknown, GenBank info on AF027217 (Revised Jul. 5, 2002). cited by other.
Author Unknown, NCBI Sequence Revision History of AF027217 (Revised Jul. 5, 2002). cited by other.
Barany, F., 1991, PNAS. USA, 88: 189-193. cited by other.
Blanchard et al., 2003, "An ORF2 Protein-Based ELISA for Procine Circovirus Type 2 Antibodies in Post-Weaning Multisystemic Wasting Syndrome", Veterinary Microbiology, 94: 183-184. cited by other.
Blanchard et al., 2003, "Protection of Swine Against Post-Weaning Multisystemic Wasting Syndrome (PMWS) by Porcine Circovirus Type 2 (PCV2) Proteins", Vaccine 21: 4565-4575. cited by other.
Buckholz, R.G., 1993, Yeast systems for the expression of heterologous gene products. Curr. Op. Biotechnology 4: 538-542. cited by other.
Burg, J.L. et al., 1996, Mol. and Cell. Probes, 10:257-271. cited by other.
Chu, B.C.F. et al., 1986, NAR, 14: 5591-5603. cited by other.
Chu, P.W.G. et al., 1993, Virus Research, 27: 161-171. cited by other.
Chu, Te-hua Terina et al., 1997, "Toward Highly Efficient Cell-Type-Specific Gene Transfer with Retroviral Vectors Displaying Single-Chain Antibodies", J. Virol., 71(1): 720-725. cited by other.
Clark, E.G., 1997, American Association of Swine Practitioners, 499-501. cited by other.
Cosset, Francois-Loic et al., 1995, "Retroviral Retargeting by Envelopes Expressing an N-Terminal Binding Domain", J. Virol., 69:6314-6322. cited by other.
Daft, B. et al., 1996, American Association of Veterinary Laboratory Diagnosticians, 32. cited by other.
Derse, D. et al., 1995, J. Virol., 69(3): 1907-1912. cited by other.
Derwent Abstract of French Patent Appl. No. 2422956, 1979. cited by other.
Derwent Abstract of French Patent Appl. No. 2518755, 1983. cited by other.
Dialog Inpadoc Family and Legal Status Search for German Patent Appl. No. 10044648, 2000. cited by other.
Dialog Inpadoc Family and Legal Status Search for U.S. Patent Appl. Pub. No. 2002/0177216, 2002. cited by other.
Dialog Inpadoc Family and Legal Status Search for U.S. Patent Appl. Pub. No. 2002/0106639, 2002. cited by other.
Dialog Inpadoc Family and Legal Status Search for U.S. Patent Appl. No. 2002/0055189, 2002. cited by other.
Dialog Inpadoc Family and Legal Status Search for U.S. Patent Appl. No. 6,517,843, 2003. cited by other.
Dialog Inpadoc Family and Legal Status Search for U.S. Patent No. 6,143,334, 2000. cited by other.
Dialog Inpadoc Family and Legal Status Search for U.S. Patent No. 6,287,856, 2001. cited by other.
Dialog Inpadoc Family and Legal Status Search for U.S. Patent No. 6,217,883, 2001. cited by other.
Dialog Inpadoc Family and Legal Status Search for US Patent No. 6,497,883, 2002. cited by other.
Dialog Inpadoc Family and Legal Status Search for WO 01/96377, 2001. cited by other.
Dialog Inpadoc Family and Legal Status Search for WO 02/102999, 2002. cited by other.
Duck, P. et al., 1990, Biotechniques, 9: 142-147. cited by other.
Dulac, G.C. et al., 1989, Can. J. Vet. Res., 53: 431-433. cited by other.
Edwards, C.P., and Aruffo, A., 1993, Current applications of COS cell based transient expression systems. Curr. Op. Biotechnology 4: 558-563. cited by other.
Edwards, S. et al., 1994, Vet. Rec., 134: 680-681. cited by other.
Ellis, J. et al., "Isolation of Circovirus from legions of Pigs with Postweaning Multisystemic Wasting Syndrome", Canadian Veterinary Journal, col. 39, pp. 44-51, Jan. 1998, XP-002068502. cited by other.
Erlich, H.A., 1989, In PCR Technology. Principles and Applications for DNA Amplification. New York: Stockton Press. cited by other.
Felgner, et al., 1987, Proc. Natl. Acad. Sci., 84: 7413-7417. cited by other.
Fontes, E.P.B. et al., 1994, J. Biol. Chem., vol. 269, No. 11: 8459-8465. cited by other.
Fraley et al., 1980, J. Biol. Chem., 255: 10431-10435. cited by other.
Guateli, J.C. et al., 1990, Proc. Nat'l Acad. Sci., USA, 87: 1874-1878. cited by other.
Hackland, A.F. et al., 1994, Arch. Virol., 139: 1-22. cited by other.
Hamel, A. et al., "Nucleotide Sequence of Procine Circovirus Associated with Postweaning Multisystemic Wasting Syndrome in Pigs", Journal of Virology, col. 72, No. 6, pp. 5262-5267, Jun. 1998, XP-002078783. cited by other.
Hanson, S.F. et al., 1995, Virology, 211: 1-9. cited by other.
Harding, J.C. and Clark, E.G., 1997, Swine Health and Production, vol. 5, No. 5: 201-203. cited by other.
Harding, J.C., 1997, American Association of Swine Practitioners, 503. cited by other.
Harding, R.M. et al., 1993, Journal of General Virology, 74: 323-328. cited by other.
Heyraud-Nitschke, F. et al., 1995, Nucleic Acids Research, vol. 23, No. 6: 910-916. cited by other.
Horner, G.W., 1991, Surveillance 18(5): 23. cited by other.
Huygen, K. et al., 1996, Nature Medicine, 2(8): 893-898. cited by other.
Innis, M.A. et al., 1990, in PCR Protocols. A guide to Methods and Applications, San Diego, Academic Press. cited by other.
Kaneda, et al., 1989, Science, 243: 375-378. cited by other.
Kasahara, Noriyuki et al., 1994, "Tissue-Specific Targeting of Retroviral Vectors Through Ligan-Receptor Interactions", Science, 266:1373-1376. cited by other.
Kessler, C., "Overview of Amplification on Systems" in Non-radioactive Labeling and Detection of Biomolecules, 1992, Springer Verlag, Berlin, New-York: 197-205. cited by other.
Kievitis, T. et al., 1991, J. Virol. Methods, 35: 273-286. cited by other.
Kohler, G. & Milstein, 1975, Nature, 256(5517): 495-497. cited by other.
Kwoh, D.Y. et al., 1989, Proc. Nat'l Acad. Sci., USA, 86: 1173-1177. cited by other.
Ladany, S. et al., 1989, J. Clin. Microbiol. 27: 2778-2783. cited by other.
Lazarowitz, S.G. et al., 1989, The EMBO Journal, vol. 8 No. 4:1023-1032. cited by other.
Liu et al., 1997, J. Gen. Virol., 78 (Pt 6), 1265-1270. cited by other.
Luckow, V.A., 1993, Baculovirus systems for the expression of human gene products. Curr. Op. Biotechnol. 4: 564-572. cited by other.
Mankertz, A. et al., 1997, J. Virol., 71: 2562-2566. cited by other.
Marglin, A. and Merrifield, R.B., 1966, J. Am. Chem. Soc., 88(21): 5051-5052. cited by other.
Matthews, J.A. et al., 1988, Anal. Biochem., 169: 1-25. cited by other.
McNeilly, F. et al., 1996, Vet. Immunol. Immunopathol., 49: 295-306. cited by other.
Meehan, B.M. et al., 1997, J. Gen. Virol. 78: 221-227. cited by other.
Midoux, 1993, Nucleic Acids Research, 21: 871-878. cited by other.
Miele, E.A. et al., 1983, J. Mol. Biol., 171: 281-295. cited by other.
Morozov, I. et al., "Detection of a Novel Strain of Procine Circovirus in Pigs with Postweaning Multisystemic Wasting Syndrome", Journal of Clinical Microbiology, col. 36, No. 9, pp. 2535-2541, Sep. 1998, XP-002090921. cited by other.
Muller, 1974, in Methode der Organischen Chemie, E. Wunsch Ed., vol. 15-I and 15-II, Thieme, Stuttgart. cited by other.
Nayar, G.P. et al., 1997, Can. Vet. J. 38(6): 385-386. cited by other.
Neddleman, Saul B. et al., 1970, "A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins", J. Mol. Biol., 48(3): 443-453. cited by other.
Olins, P.O., and Lee, S.C., 1993, Curr. Op. Biotechnol. 4: 520-525. cited by other.
Pagano et al., 1967, J. Virol., 1: 891-897. cited by other.
Pearson, William R. et al., 1988, "Improved Tools for Biological Sequence Comparison", Proc. Nat'l Acad, Sci., USA, 85: 2444-2448. cited by other.
Rolfs, A. et al., "Usage of Polymerase Chain Reaction in Genetic and Infectious Disease" in PCR Topics, 1991, Springer-Verlag, Berlin. cited by other.
Rose, N. et al., 2002, "Risk Factors for Porcine Post-Weaning Multisystemic Wasting Syndrome (PMWS) in 149 French Farrow-to-Finish Herds", Preventive Veterinary Microbiology, 61: 209-225. cited by other.
Sambrook, J. et al., 1989, Molecular cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. cited by other.
Sanchez-Pescador, R., 1988, J. Clin. Microbiol., 26(10): 1934-1938. cited by other.
Segales, J. et al., "First Report of Post-Weaning Multisystemic Wasting Syndrome in Pigs in Spain", Beterinary Record, col. 141, No. 23, pp. 600-601, Dec. 1997, XP-002068504. cited by other.
Shiver, J.W., "Immune Responses to HIV gp120 Elicited by DNA Vaccination," in Vaccines, 1995, eds Chanock, et al., pp. 95-98, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. cited by other.
Smith, Temple F. et al., 1981, "Comparison of Biosequences", Advances in Applied Mathematics, 2: 482-489. cited by other.
Tascon, R.E. et al., 1996, Nature Medicine, 2(8): 888-892. cited by other.
Tischer, I. and Bohk, H.J., 1988, Zentralbl Bakteriol Mikrobiol Hyg [A] 270: 280-287. cited by other.
Tischer, I. et al., 1982, Nature, 295: 64-66. cited by other.
Tischer, I. et al., 1986, Arch. Virol., 91: 271-276. cited by other.
Tischer, I. et al., 1995, Arch. Virol., 140: 737-743. cited by other.
Urdea, M.S., 1988, Nucleic Acids Research, 11:4937-4957. cited by other.
Valsesia-Whittmann, Sandrine et al., 1996, "Improvement of Retroviral Retargeting by Using Amino Acid Spacers between an Additional Binding Domain and the N Terminus of Moloney Nurine Leukemia Virus SU", J. Virol, 70(3): 2059-2064. cited by other.
Virus Taxonomy, Murphy, F.A. et al., Ed. 1995, Sixth Report of the International Committee on Taxonomy of Viruses, Springer-Verlag Wien New York. cited by other.
Walker, G.T. et al., 1992, Nucleic Acids Res. 20:1691-1696. cited by other.
Walker, G.T. et al., 1992, Proc. Nat'l Acad. Sci., USA, 89: 392-396. cited by other.
White, B.A. et al. Eds, "PCR Cloning Protocol" in Methods in Molecular Biology, 67, Humana Press, Towota, 1997. cited by other.
Young, John A. T. et al., "Efficient Incorporation of Human CD4 Protein into Avian Leukosis Virus Particles", Reports, 1421-1423, 1990. cited by other.
Zhao, T.M. et al., 1996, Proc. Natl. Acad. Sci., USA 93(13): 6653-6648. cited by other.
Opposition against EP 1 036 180 B1 filed by Boehringer Ingelheim on Jul. 24, 2009 ("Opposition") (57 pages). cited by other.
Notification of Opposition against EP 1 036 180 B1 dated Aug. 11, 2009 (1 page). cited by other.
"D1" from Opposition - "WO 99/18214 (English translation)" (38 pages). cited by other.
"D2" from Opposition - "Submission of the Proprietor to the EPO dated Apr. 11, 2008" (4 pages). cited by other.
"D3" from Opposition - "Translation of SEQ ID No:4 of WO 99/18214 (PCV IMP1010)" (5 pages). cited by other.
"D4" from Opposition - "Printout of SEQ ID No:4 from Patent WO 99/18214 (GenBank A97281.1)" (1 page). cited by other.
"D5" from Opposition - "Translation of SEQ ID No:1 of WO 99/18214" (5 pages). cited by other.
"D6" from Opposition - "Translation of SEQ ID No:2 of WO 99/18214" (5 pages). cited by other.
"D7" from Opposition - "Printout of SEQ ID No:1 from Patent WO 99/18214 (GenBank A97278.1)" (1 page). cited by other.
"D8" from Opposition - "Printout of SEQ ID No:2 from Patent WO 99/18214 (GenBank A97279.1)" (1 page). cited by other.
"D9" from Opposition - "Translation of SEQ ID No:3 of WO 99/18214" (5 pages). cited by other.
"D10" from Opposition - "Printout of SEQ ID No:3 from Patent WO 99/18214 (GenBank A97280.1)" (1 page). cited by other.
"D11" from Opposition - "Translation of SEQ ID No:1 from Patent WO 99/18214" (5 pages). cited by other.
"D12" from Opposition - "Printout of SEQ ID No:6 from Patent WO 99/18214 (GenBank A97283.1)" (1 page). cited by other.
"D13" from Opposition - "WO 99/29717" (82 pages). cited by other.
"D13a" from Opposition - "U.S. Appl. No. 60/069,750 (priority document dated Dec. 16, 1997 of D13)" (46 pages). cited by other.
"D14" from Opposition - "Marked Figures pp. 3/12, 4/12 and 6/12 of D13" (3 pages). cited by other.
"D15" from Opposition - "Hamel, A., et al., J. Virol., Jun. 1998, pp. 5262-5267" (6 pages). cited by other.
"D16" from Opposition - "Enlarged excerpt of p. 5265 of D15" (1 page). cited by other.
"D17" from Opposition - "Translation of pmws nucleotide sequence in D15" (5 pages). cited by other.
"D18" from Opposition - "Printout of nucleotide sequence in D15 retrieved from GenBank (AF27217.1)" (3 pages). cited by other.
"D19" from Opposition - "Meehan et al., J. Gen. Virol., Sep. 1998, 79, 2171-2179" (9 pages). cited by other.
"D19a" from Opposition - "Sequence alignments - Sequences in the opposed patent and in D19" (8 pages). cited by other.
"D19b" from Opposition - "Sequence alignments - Sequences in the opposed patent and in D19" (26 pages). cited by other.
"D19c" from Opposition - "Sequence alignments - Sequences in the opposed patent and in D15" (3 pages). cited by other.
"D20" from Opposition - "Morozov et al., J. Clin. Microbiol., Sept. 1998, p. 2535-2541" (7 pages). cited by other.
"D21" from Opposition - "Nucleotide sequence retrieved from GenBank (deposited as AJ 223185)" (2 pages). cited by other.
"D22" from Opposition - "Translation of nucleotide sequence in D21"(5 pages). cited by other.
"D23" from Opposition - "Ellis et al., Canadian Veterinarian Journal, vol. 39, Jan. 1998" (8 pages). cited by other.
"D24" from Opposition - "Allan et al., Journal of Veterinary Diagnostic Investigation, 10:3-10 (1998)" (8 pages). cited by other.
"D25" from Opposition - "Allan et al.; Veterinary Record, 1998, p. 467 to 468" (2 pages). cited by other.
"D26" from Opposition - "English translation of the Opposed Patent EP 1 036 180 B1" (81 pages). cited by other.









Abstract: The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided. Methods for detecting the nucleic acids or polypeptides, and kits for diagnosing infection by a PWD circovirus, also are provided. Method for selecting compounds capable of modulating the viral infection are further provided. Pharmaceutical, including vaccine, compositions for preventing and/or treating viral infections caused by PWD circovirus and the use of vectors for preventing and/or treating diseases also are provided.
Claim: We claim:

1. A vaccine comprising an isolated nucleic acid having a nucleotide sequence with at least 90% sequence identity to SEQ ID NO:15 and an acceptable pharmaceutical vehicle, wherein saidnucleic acid encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease circovirus, wherein said nucleotide sequence comprises a sequence having at least 90% sequence identity to SEQ IDNO:25 and SEQ ID NO:27.

2. An isolated immunogenic porcine circovirus Type B (PCVB) polypeptide having at least 90% identity to the sequence of SEQ ID NO: 26, wherein the polypeptide induces an immunological response in a porcine against PCVB.

3. A vaccine comprising an isolated nucleic acid having a nucleotide sequence with at least 90% sequence identity to SEQ ID NO:15 and an acceptable pharmaceutical vehicle, wherein said nucleic acid encodes an immunogenic protein that induces aprotective response effective against infection by a piglet weight loss disease circovirus, wherein said nucleotide sequence comprises a sequence having at least 95% sequence identity to SEQ ID NO:25 and SEQ ID NO:27.

4. A vaccine comprising an isolated nucleic acid having a nucleotide sequence with at least 95% sequence identity to SEQ ID NO:15 and an acceptable pharmaceutical vehicle, wherein said nucleic acid encodes an immunogenic protein that induces aprotective response effective against infection by a piglet weight loss disease circovirus, wherein said nucleotide sequence comprises a sequence having at least 90% sequence identity to SEQ ID NO:25 and SEQ ID NO:27.

5. A vaccine comprising an isolated nucleic acid having a nucleotide sequence with at least 95% sequence identity to SEQ ID NO:15 and an acceptable pharmaceutical vehicle, wherein said nucleic acid encodes an immunogenic protein that induces aprotective response effective against infection by a piglet weight loss disease circovirus, wherein said nucleotide sequence comprises a sequence having at least 95% sequence identity to SEQ ID NO:25 and SEQ ID NO:27.

6. A vaccine according to claim 1, claim 3, claim 4, or claim 5, wherein said nucleotide sequence is SEQ ID NO:15.

7. The vaccine of claim 1, claim 3, claim 4, or claim 5, further comprising an adjuvant.

8. The vaccine of claim 1, claim 3, claim 4, or claim 5, wherein said sequence consists essentially of SEQ ID NO:15.

9. An isolated immunogenic porcine circovirus Type B (PCVB) polypeptide having at least 95% identity to the sequence of SEQ ID NO: 26, wherein the polypeptide induces an immunological response in a porcine against PCVB.

10. The immunogenic PCVB polypeptide of claim 9 having at least 98% identity to SEQ ID NO: 26.

11. The immunogenic PCVB polypeptide of claim 9 having the sequence of SEQ ID NO: 26.

12. A composition comprising the immunogenic PCVB polypeptide of any one of claims 9 to 11 and a pharmaceutically or veterinarily acceptable carrier.

13. The composition of claim 12, further comprising an adjuvant.

14. The isolated polypeptide of any one of claims 9 to 11 and claim 2, wherein the polypeptide is generated by recombinant production.

15. The isolated polypeptide of any one of claims 9 to 11 and claim 2, wherein the polypeptide is generated by chemical synthesis.

16. A method of immunizing a mammal against piglet weight loss disease comprising administering to a mammal an effective amount of the vaccine of claim 1, claim 3, claim 4, or claim 5.

17. The method according to claim 16, further comprising an adjuvant.

18. A method for detecting PCVB homologous sequences in a biological sample comprising: (a) incubating the biological sample with a polynucleotide that specifically hybridizes to a PCVB nucleotide sequence but not to polynucleotides from othermembers of the circovirus family, wherein the polynucleotide comprises at least 8 contiguous nucleotides from, or complementary to, a PCVB sequence having SEQ ID NO:15, under conditions which promote the formation of nucleic acid complexes between thepolynucleotide and the PCVB nucleotide sequence present in the biological sample; and (b) detecting the complexes containing the polynucleotide.

19. The method of claim 18 wherein said polynucleotide is labeled, and the complexes are detected by detection of the presence of the label.

20. The method of claim 18, wherein said detecting comprises using two PCVB nucleic acid specific probes wherein the two probes are selected from the group of probe pairs consisting of SEQ ID NO:46 and SEQ ID NO:47, SEQ ID NO:46 and SEQ IDNO:48, SEQ ID NO:49 and SEQ ID NO:46, SEQ ID NO:49 and SEQ ID NO:48, and SEQ ID NO:50 and SEQ ID NO:51, converting the nucleic acid/probe hybridization complexes to double-strand probe-containing fragments by primer extension reactions, amplifying thenumber of probe-containing fragments by successively repeating the steps of (i) denaturing the double-strand fragments to produce single-strand fragments, (ii) hybridizing the single strands with the probes to form strand/probe complexes, (iii)generating double-strand fragments from the strand/probe complexes in the presence of DNA polymerase and all four deoxyribonucleotides, and (iv) repeating steps (i) to (iii) until a desired degree of amplification has been achieved, identifying theamplification products.

21. The method of claim 18, wherein the polynucleotide is at least 10 nucleotides in length.

22. The method of claim 18, wherein the polynucleotide is at least 25 nucleotides in length.

23. The method of claim 18 wherein the polynucleotide is at least 75 nucleotides in length.
Description:
 
 
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