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Eukaryotic translation initiation factor gene regulatory elements for use in plants |
| 7507876 |
Eukaryotic translation initiation factor gene regulatory elements for use in plants
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| Patent Drawings: | |
| Inventor: |
Flasinski, et al. |
| Date Issued: |
March 24, 2009 |
| Application: |
11/743,923 |
| Filed: |
May 3, 2007 |
| Inventors: |
Flasinski; Stanislaw (Chesterfield, MO)
Screen; Steven E. (St. Louis, MO)
Feeney; Deborah (Warrenton, MO)
Cerny; Richard Eric (Chesterfield, MO)
Hart; Jessie (Augusta, MO)
Malven; Marianne (Ellisville, MO)
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| Assignee: |
Monsanto Technology LLC (St. Louis, MO) |
| Primary Examiner: |
Bui; Phuong T |
| Assistant Examiner: |
Kumar; Vinod |
| Attorney Or Agent: |
Sonnenschein Nath & Rosenthal LLP |
| U.S. Class: |
800/298; 435/320.1; 435/419; 435/468; 536/24.1; 800/300 |
| Field Of Search: |
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| International Class: |
A01H 1/00; C07H 21/04; C12N 15/09; C12N 5/14 |
| U.S Patent Documents: |
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| Foreign Patent Documents: |
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| Other References: |
Kim et al. (Plant Molecular Biology, 24:105-117, 1994). cited by examiner.
Donald et al. (EMBO J. 9:1717-1726, 1990). cited by examiner.
Benfey et al., (Science 250:959-966, 1990). cited by examiner.
Mandel et al. (Plant Molecular Biology, 29:995-1004, 1995). cited by examiner.
Jiang et al. (Crop Science, 40:1729-1741, 2000). cited by examiner.
Christensen et al. (Transgenic Research, 5:213-218, 1996). cited by examiner.
Pautot et al. (Gene 77:133-140, 1989). cited by examiner.
Guarente et al. (TIG, 8:27-32, 1992). cited by examiner.
Maniatis et al. (Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, 1982). cited by examiner.
Brander et al.; "Highly conserved genes coding for eukaryotic translation initiation factor eIF-4A of tobacco have specific alterations in functional motifs," Biochimica et Biophysica Acta, 1261:442-444, 1995. cited by other.
Database NCBI, GI:14594801, Database Accession No. AJ298137, Apr. 15, 2005. cited by other.
Database NCBI, GI:475215, Database Accession No. X79008, Apr. 18, 2005. cited by other. |
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| Abstract: |
The present invention provides eukaryotic translation initiation factor non-coding regulatory element polynucleotide molecules isolated from Nicotiana tabacum, Arabidopsis thaliana, and Medicago truncatula useful for modulating transgene expression in plants. The present invention also provides expression constructs containing the polynucleotide molecules useful for modulating transgene expression in plants. The present invention also provides transgenic plants and seeds containing the polynucleotide molecules useful for modulating transgene expression in plants. |
| Claim: |
We claim:
1. An isolated polynucleotide molecule having promoter activity, wherein said molecule is selected from the group consisting of: a. a DNA molecule comprising SEQ ID NO: 7; and b. afragment comprising at least 95 contiguous nucleotide bases of SEQ ID NO: 7 having promoter activity.
2. The isolated polynucleotide molecule of claim 1 comprising SEQ ID NO: 7.
3. A polynucleotide construct comprising the isolated polynucleotide molecule of claim 1, wherein said isolated polynucleotide molecule is operably linked to a transcribable polynucleotide molecule.
4. The polynucleotide construct of claim 3, wherein the isolated polynucleotide molecule comprises the nucleic acid sequence of SEQ ID NO: 7.
5. The polynucleotide construct of claim 3, wherein said transcribable polynucleotide molecule is a gene of agronomic interest.
6. The polynucleotide construct of claim 3, wherein said transcribable polynucleotide molecule is a herbicide tolerance gene.
7. The polynucleotide construct of claim 6, wherein said herbicide tolerance gene is selected from the group consisting of genes that encode for phosphinothricin acetyltransferase, glyphosate resistant EPSPS, hydroxyphenyl pyruvatedehydrogenase, dalapon dehalogenase, bromoxynil resistant nitrilase, anthranilate synthase, glyphosate oxidoreductase and glyphosate-N-acetyl transferase.
8. A transgenic plant cell stably transformed with the polynucleotide construct of claim 3.
9. A transgenic plant stably transformed with the polynucleotide construct of claim 3.
10. The transgenic plant of claim 9, wherein the polynucleotide construct comprises SEQ ID NO: 7.
11. The transgenic plant of claim 9 or 10, wherein said plant is a monocotyledonous plant selected from the group consisting of wheat, maize, rye, rice, oat, barley, turfgrass, sorghum, millet and sugarcane.
12. The transgenic plant of claim 9 or 10, wherein said plant is a dicotyledonous plant selected from the group consisting of tobacco, tomato, potato, soybean, cotton, canola, sunflower and alfalfa.
13. A seed of said transgenic plant of claim 11, wherein said seed comprises said polynucleotide construct.
14. A seed of said transgenic plant of claim 12, wherein said seed comprises said polynucleotide construct.
15. A method of inhibiting weed growth in a field of transgenic glyphosate tolerant crop plants comprising: (i) planting transgenic plants transformed with an expression cassette comprising the isolated polynucleotide molecule of claim 1operably linked to a polynucleotide molecule encoding a glyphosate tolerance protein and (ii) applying glyphosate to the field at an application rate that inhibits the growth of weeds, wherein the growth and yield of the transgenic crop plant is notsubstantially affected by the glyphosate application.
16. The method of claim 15, wherein said glyphosate tolerance protein is selected from the group consisting of a glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a glyphosate oxidoreductase and a glyphosate-N-acetyltransferase.
17. The method of claim 15, wherein the transgenic plants are capable of tolerating an application rate ranging up to 256 ounces/acre.
18. The method of claim 15, wherein the application of glyphosate is at least once during the growth of the crop. |
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