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Medicinal compositions containing aspirin |
| 7371760 |
Medicinal compositions containing aspirin
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| Patent Drawings: | |
| Inventor: |
Asai, et al. |
| Date Issued: |
May 13, 2008 |
| Application: |
10/600,266 |
| Filed: |
June 20, 2003 |
| Inventors: |
Asai; Fumitoshi (Nishitokyo, JP)
Sugidachi; Atsuhiro (Kawasaki, JP)
Ogawa; Taketoshi (Tokyo, JP)
Inoue; Teruhiko (Ube, JP)
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| Assignee: |
Sankyo Company, Limited (Tokyo, JP) |
| Primary Examiner: |
Kwon; Brian |
| Assistant Examiner: |
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| Attorney Or Agent: |
Frishauf, Holtz, Goodman & Chick, P.C. |
| U.S. Class: |
514/301; 514/161; 514/165 |
| Field Of Search: |
514/301; 514/259.3; 514/310; 514/165; 514/259.03 |
| International Class: |
A61K 31/60; A61K 31/4743 |
| U.S Patent Documents: |
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| Foreign Patent Documents: |
0 542 411; 1 298 132; 6-41139; 2000-205396; 2000-266780; 2002-145883; WO 97/29753 |
| Other References: |
Sugidachi et al., "The in vivo pharmacological profile of CS-747, a novel antiplatelet agent with platelet ADP receptor antogonistproperties", British Journal of Pharmacology, 2000, vol. 209, No. 7, pp. 1439 to 1446. cited by other.
Database CA on STN, AN.133:187474, ASAI, Fumitoshi et al., CS-747, a new platelet ADP receptor antagonist Annual Report of Sankyo Research Laboratories, 1999, vol. 51, pp. 1 to 44, abstract. cited by other.
Saniabadi AR et al., "Effect of dipyridamole alone and in combination with aspirin on whole blood platelet aggregation, PGI2 generation, and red cell deformability ex vivo in man", Cardiovascular Research, 1991, vol. 25, No. 3, pp. 177 to 183. citedby other.
J. Cardiovasc. Pharmacol., vol. 49, No. 3, Mar. 2007, p. 167-173. cited by other.
Seminars in Thrombosis and Hemostasis, vol. 31, No. 2, 2005, p. 184-194. cited by other. |
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| Abstract: |
A combination of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, and aspirin, which possess excellent inhibitory activity against platelet aggregation and thrombogenesis, and is useful for preventing or treating diseases caused by thrombus or embolus. |
| Claim: |
What is claimed is:
1. A pharmaceutical composition consisting essentially of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or apharmaceutically acceptable salt thereof, and aspirin, in a ratio by weight of 1:500 to 500:1 and, optionally, a pharmaceutically acceptable excipient.
2. The pharmaceutical composition of claim 1 containing said pharmaceutically acceptable excipient.
3. The pharmaceutical composition of claim 1 wherein the 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine is in the form of a pharmaceutically acceptable salt.
4. The pharmaceutical composition according to claim 3, wherein the pharmaceutically acceptable salt is the hydrochloride.
5. The pharmaceutical composition according to claim 3, wherein the pharmaceutically acceptable salt is the maleate. |
| Description: |
BACKGROUND OF THE INVENTION
This invention relates to pharmaceutical compositions containing 2-acetoxy-5(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7tetrahydro- thieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, and aspirin, as active ingredients[particularly pharmaceutical compositions for prevention or treatment (particularly for treatment) of diseases caused by thrombus or embolus]; to the use of 2-acetoxy-5-(.alpha.cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahydr- othieno[3,2-c]pyridineor a pharmaceutically acceptable salt thereof and aspirin for the manufacture of pharmaceutical compositions for prevention or treatment (particularly for treatment) of diseases caused by thrombus or embolus; and to methods for the prevention ortreatment (particularly to methods for the treatment) of diseases caused by thrombus or embolus by administration of an effective amount of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or apharmaceutically acceptable salt thereof and aspirin to warm-blooded animals (particularly humans).
2-Acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahydr- othieno[3,2-c]pyridine has been described in the Japanese Patent Application Publication No. Hei 6-41139, and possesses potent inhibitory activity against plateletaggregation. Furthermore, aspirin is well known to have an inhibiting activity against platelet aggregation, although the activity is low. However, pharmaceutical compositions containing both compounds have not been known.
BRIEF DESCRIPTION OF THE INVENTION
The present inventors have studied therapeutic agents with low toxicity that exert inhibitory activity against platelet aggregation and have found that the problems described above are solved by using pharmaceutical compositions comprising2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof and aspirin.
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides pharmaceutical compositions containing 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof and aspirin as activeingredients [particularly pharmaceutical compositions for prevention or treatment (particularly for treatment) of diseases caused by thrombus or embolus]; the use of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd-rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, and aspirin, for the manufacture of pharmaceutical compositions [particularly pharmaceutical compositions for prevention or treatment (particularly for treatment) of diseases causedby thrombus or embolus]; and methods for the prevention or treatment (particularly methods for treatment) of diseases caused by thrombus or embolus by administration of an effective amount of2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,- 7-tetrahydrothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, and aspirin, to warm-blooded animals (particularly humans), simultaneously or sequentially.
2-Acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahydr- othieno[3,2-c]pyridine, and pharmaceutically acceptable salts thereof, which is one of the active ingredients of the present invention, is a known compound. Forinstance, the compound has already been described in Japanese Patent Application Publication No. Hei 6-41139 and Japanese Patent Application Publication No. 2002-145882 (priority: Japanese Patent Application No. 2000-205539, and Japanese PatentApplication No. 2000-266780). The chemical structure is described below.
##STR00001##
The pharmaceutically acceptable salts of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine may be, for example, hydrohalogenic acid salts such as hydrofluoride, hydrochloride, hydrobromide orhydroiodide; nitrate; perchlorate; sulfate; phosphate; C.sub.1-C.sub.4 alkanesulfonates optionally substituted by halogens such as methanesulfonate, trifluoromethanesulfonate, ethanesulfonate; C.sub.6-C.sub.10 arylsulfonates optionally substituted byC.sub.1-C.sub.4 alkyl groups such as benzenesulfonate or p-toluenesulfonate; C.sub.1-C.sub.6 aliphatic acid salts such as acetate, malate, fumarate, succinate, citrate, tartarate, oxalate or maleate; amino acid salts such as glycine salt, lysine salt,arginine salt, ornitine salt, glutamic acid salt or aspartic acid salt; and the preferred compounds are hydrohalogenates or C.sub.1-C.sub.6 aliphatic acid salts; and more preferred compounds are the hydrochloride or the maleate.
When one of the active ingredients of the present invention, 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, is allowed to stand so that it is opento the atmosphere, it may become hydrated by absorption of water or adsorption of water. Such hydrated compounds are included in the present invention.
Further, one of the active ingredients of the present invention, 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, may absorb some kinds of organicsolvents and may form solvates in some cases, and these solvates are also included in the present invention.
Furthermore, since 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine has an asymmetric carbon atom, optical isomers exist based on the asymmetric carbon atom. These optical isomers are alsoincluded in the present invention.
The other active ingredient, aspirin, is a well-known compound, as an analgesic antipyretic.
The pharmaceutical compositions of the present invention (particularly pharmaceutical compositions for the prevention or treatment of diseases caused by thrombus or embolus) which contain2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, and aspirin, as active ingredients, possess excellent inhibitory activity against platelet aggregation andthrombogenesis with short onset latency and low toxicity. Thus the pharmaceutical compositions of the present invention are useful as preventative or therapeutic agents (particularly as therapeutic agents) against diseases caused by thrombus or embolus,for example, diseases induced by platelet aggregation, including stable or unstable angina pectoris and so forth; cardiovascular or cerebrovascular disorders, e.g., thromboembolism, associated with atherosclerosis or diabetes mellitus, such as unstableangina pectoris, cerebral ischemic insult or restenosis due to angioplasty, endarterectomy or stent therapy; or thromboembolism caused by thromboembolization such as recurrent embolism after degradation of the original thrombus, embolism,ischemia-induced dementia, peripheral arteriopathy, thromboembolization associated with hemodialysis or atrial fibrillation, or thromboembolization in the vascular prosthesis, or in the bypass between the aorta and the coronary artery. Furthermore, thetherapeutic agent of the present invention is administered to warm-blooded animals (particularly humans).
According to the present invention, the use in combination of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, and aspirin, results in more potenteffectiveness than the use of each component alone. Furthermore, plasma levels of these agents do not have to be maintained at a certain level and higher during the same period, in order to produce their effects. It is believed that these 2 agentsreach the receptors, at which they act in vivo, and turn on switches at the receptors to induce the effects. Even though the plasma level of one component of the pharmaceutical composition is too low to induce the effects with increasing time after theagent was administered, the switches at the receptors have already been turned on. Thus the preventative or therapeutic efficacy of the agent is expected by inhibiting thrombogenesis or embolization.
Therefore, when the other component of the pharmaceutical composition is administered later, the therapeutic effect of the compound administered later is expected to add to the therapeutic effects of the previously administered component. However, it is convenient clinically that both components are administered at the same time. Thus 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof andaspirin are simultaneously administered as a combination drug. In the case that both agents cannot be mixed technically, each component can be administered separately. Moreover, as described previously, since each component produces significant effectsas a single form, each component can be sequentially administered at appropriate intervals. The maximum intervals between administration of each of the two components that can be accepted to elicit significant effects could be confirmed by clinicaltrials or animal studies.
The route for administration of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or a pharmaceutically acceptable salt thereof, and aspirin, which is employed in the present invention, isgenerally the oral route. However, other routes, for example, intravenous administration, can be used. Thus, the 2 components can be prepared respectively as separate formulations, or can be mixed physically to form a single formulation foradministration. The single formulations of the mixed components are, for example, powders, granules, tablets, capsules and so forth, and can be prepared by regular formulation techniques, as described below.
These formulations are prepared by conventional methods by using excipients (organic excipients, for example, sugar derivatives such as lactose, sucrose, glucose, mannitol or sorbitol; starch derivatives such as corn starch, potato starch,.alpha.-starch or dextrin; cellulose derivatives such as crystalline cellulose; gum arabic; dextran; or pullulan; and inorganic excipients, for example, silicate derivatives such as light silicic acid anhydride, synthetic aluminum silicate, calciumsilicate or magnesium aluminate metasilicate; phosphate derivatives such as calcium hydrogenphosphate; carbonates such as calcium carbonate; or sulfates such as calcium sulfate), lubricants (for example, stearic acid; metal stearate derivatives such ascalcium stearate or magnesium stearate; talc; waxes such as beeswax or spermaceti; boric acid; adipic acid; sulfate derivatives such as sodium sulfate; glycol; fumaric acid; sodium benzoate; DL-leucine; lauryl sulfate derivatives such as sodium laurylsulfate or magnesium lauryl sulfate; silicic acid derivatives such as silicic acid anhydride or silicic acid hydrate; and starch derivatives described above), binders (for example, hydroxypropyl cellulose, hydroxypropylmethylcellulose,poly(vinylpyrrolidone), polyethylene glycol and similar compounds described in the above excipients), disintegrators (for example, cellulose derivatives such as low substituted hydroxypropylcellulose, carboxymethylcellulose, calciumcarboxymethylcellulose, internally cross-linked sodium carboxymethylcellulose; chemically modified starch/cellulose derivatives such as carboxymethylstarch, sodium carboxymethylstarch; cross-linked polyvinylpyrrolidone; or starch derivatives describedabove), emulsifiers (for example, colloidal clays such as bentonite or veegum; metal hydroxides such as magnesium hydroxide or aluminum hydroxide; anionic surfactants such as sodium lauryl sulfate or calcium stearate; cationic surfactants such asbenzalkonium chloride; or nonionic surfactants such as polyoxyethylene alkyl ether, polyoxyethylenesorbitan ester of fatty acids or sucrose ester of fatty acids), stabilizers (for example, parahydroxybenzoates such as methylparaben or propylparaben;alcohols such as chlorobutanol, benzyl alcohol or phenylethyl alcohol; benzalkonium chlorides; phenol derivatives such as phenol or cresol; thimerosal; dehydroacetic acid; or sorbic acid), corrigents (for example, sweetening, souring and flavoring agentsall of which are conventionally used), and diluents.
The dose and the dose ratio of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or pharmaceutically acceptable salt thereof, and aspirin, can be widely altered based on several factors such asactivity of each compound, and the symptoms, age and body weight of the patients.
Generally, the lower limit of the oral dose (mg drug dose/time) is 0.1 mg (preferably, 1 mg) per time, while the upper limit is 1,000 mg (preferably, 500 mg) per time. The lower and upper limits of intravenous injection are 0.01 mg (preferably,0.1 mg) and 500 mg (preferably, 250 mg), respectively. They are administered to the adult from 1 to 7 times a day based on the symptoms of the patient, simultaneously or sequentially.
Generally, the dose ratio of 2-acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine or pharmaceutically acceptable salt thereof, and aspirin, is from 1:500 to 500:1 as their weight ratio.
EXAMPLES
The present invention is described in detail with examples and formulations in the following. However, the claim of the present invention is not restricted to the following description.
Example 1
Inhibitory Activity Against Thrombogenesis
As the test animals, male Sprague Dawley rats of 7 weeks old were purchased from SLC Japan and 6 rats per group were used.
2-Acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahydr- othieno[3,2-c]pyridine was synthesized according to the method described in the Specification of Japanese Patent Application Publication No. Hei 6-41139 and was used,while aspirin was purchased from Sigma Chemical Co. and was used. Both compounds were suspended in 5% (w/v) gum arabic solution, and were diluted so as to be 1 ml/kg of administration volume and were orally administered.
The inhibitory activities of the compounds against thrombogenesis or thrombus formation were evaluated in the modified arterio-venous shunt thrombosis model in rats, which was described by Umetsu et al. [Thromb. Haemost., 39, 74-83 (1978)].
The shunt tube was prepared as follows; i.e., both sides of a medical silicon tube of 12 cm length [inner diameter: 1.5 mm, outer diameter: 2.5 mm, purchased from KANEKA Medix Co., Ltd] were connected each to a polyethylene tube of 7 cm length[inner diameter: 0.5 mm, outer diameter: 1.0 mm, purchased from Natsume Seisakusho Co., Ltd.] covered with silicon via a medical silicon tube of 0.7 cm length [inner diameter: 1.0 mm, outer diameter: 1.5 mm, KANEKA Medix Co., Ltd] as connector. Asurgical suture of 10 cm length was placed in the silicon tube of 12 cm length.
The animal was anesthetized with an intraperitoneal injection of 40 mg/kg of pentobarbital sodium (purchased from Abbott Laboratories Inc.), and the jugular of one side and the carotid of the other side were exposed. The arteriovenous shunt wasmade by cannulation of a shunt tube filled with heparin solution [30 units/kg, purchased from Fuso Pharmaceutical Co., Ltd] into the carotid and the jugular which had been previously exposed.
The test compounds were orally administered and the blood was started to circulate into the shunt area two hours after the administration. Thirty minutes after the circulation was started, the shunt tube was removed, and the thrombus adsorbed onthe surgical suture was weighed. The results are shown in Table 1. The results in the table are expressed as the average weight.+-.SE (n=6).
TABLE-US-00001 TABLE 1 Compounds Compound A Aspirin Thrombus Weight Inhibition Rate (mg/kg) (mg/kg) (mg) (%) 0 0 52.3 .+-. 1.2 -- 0 10 46.6 .+-. 2.8 12.3 .+-. 4.4 0.3 0 43.5 .+-. 2.1 17.0 .+-. 4.1 0.6 0 37.5 .+-. 2.1 28.3 .+-. 4.0 0.3 1030.5 .+-. 3.5 41.8 .+-. 6.6 0.6 10 23.2 .+-. 3.8 55.7 .+-. 7.2 Compound A: 2-Acetoxy-5-(.alpha.-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahyd- rothieno[3,2-c]pyridine
TABLE-US-00002 (Formulation 1) Tablets Compound A 10.0 mg Aspirin 12.5 mg Lactose 175.5 mg Corn starch 50.0 mg Magnesium stearate 2.0 mg Total 250 mg
The powders in the formula described in the above table are mixed, compressed with a tableting machine and formulated as a tablet containing 250 mg in total. The tablet can be coated with film or sugar, when necessary.
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