| Patent Number |
Title Of Patent |
Date Issued |
| 5240848 |
DNA sequences encoding human vascular permeability factor having 189 amino acids |
August 31, 1993 |
| A cDNA having a base sequence for human vascular permeability factor has been cloned and characterized and the amino acid sequence of the human vascular permeability factor protein has been determined. |
| 5134065 |
Tissue plasminogen activator inhibitor and method of purification |
July 28, 1992 |
| A novel and unique plasminogen activator inhibitor fragment is obtained from human umbilical vein endothelial cells which has the following characteristics:A. it is derived from a native t-PA inhibitor that binds to and inhibits the activity of t-PA,B. it is dissociated from a complex fo |
| 5132214 |
Large scale production of plasminogen activator from normal human colon cells |
July 21, 1992 |
| Plasminogen activators (PA) are obtained from cultured normal human colon cells which are adaptable to large scale production. A purified tissue PA (t-PA) is obtained from CCD-18Co normal human colon fibroblast cells which shows chemical differences from Bowes melanoma t-PA. |
| 5036003 |
Antibodies to VPF |
July 30, 1991 |
| A method of producing antibodies against vascular permeability factor (VPF) is disclosed which comprises immunizing a host animal with a peptide having an amino acid sequence as follows: ##STR1## or a fragment of said peptide containing an antigenic determinant of VPF. |
| 5008196 |
Stimulation of endothelial cell growth |
April 16, 1991 |
| A method of stimulating endothelial cell growth is provided which comprises subjecting said cells to a growth stimulating amount of a highly purified vascular permeability factor having the following characteristics:(a) it has a M.sub.r about 34,000-40,000 as determined by sodium dodecyl |
| 4957863 |
Method of increasing yield of t-PA in cell culture |
September 18, 1990 |
| A method is disclosed for increasing the yield of t-PA in culture of mammalian cells comprising introducing antibodies to the t-PA of said cells into the cell culture nutrient medium, allowing the cells to grow, and then recovering t-PA from the t-PA-antibody complex thus found in the |
| 4927630 |
Tissue plasminogen activator from normal human colon cells |
May 22, 1990 |
| Normal human colon fibroblast tissue plasminogen activator is separated by lysine-Sepharose affinity chromatography into Types I and II glycoforms and characterized with respect to the relative incidence of each type of oligosaccharide comprising the respective Types I and II glycoforms. |
| 4891312 |
Monoclonal antibody specific for human colon fibroblast-derived t-PA |
January 2, 1990 |
| A monoclonal antibody is disclosed which is specific for human colon fibroblast-derived tissue plasminogen activator (t-PA), and which is useful for immunoaffinity chromatography purification of t-PA and determination of t-PA in a biological sample. |
| 4889922 |
Monoclonal antibody specific for human colon fibroblast-derived T-PA |
December 26, 1989 |
| A monoclonal antibody is disclosed which is specific for human colon fibroblast-derived tissue plasminogen activator (t-PA), and which is useful for immunoaffinity chromatography purification of t-PA and determination of t-PA in a biological sample. |
| 4851517 |
Tissue plasminogen activator oligosaccharide from normal human colon cells |
July 25, 1989 |
| Glycosylated plasminogen activator (t-PA) having a glycosylation pattern significantly different than exhibited by t-PA from Bowes melanoma cells is obtained from cultured normal human colon fibroblast cells (CCD-18Co). |
| 4833085 |
Monoclonal antibody specific for human colon fibroblast-derived t-PA |
May 23, 1989 |
| A monoclonal antibody is disclosed which is specific for human colon fibroblast-derived tissue plasminogen activator (t-PA), and which is useful for immunoaffinity chromatography purification of t-PA and determination of t-PA in a biological sample. |
| 4751084 |
Tissue plasminogen activator from normal human colon cells |
June 14, 1988 |
| Glycosylated plasminogen activator (t-PA) having a glycosylation pattern significantly different than exhibited by t-PA from Bowes melanoma cells is obtained from cultured normal human colon fibroblast cells (CCD-18Co). |
| 4596675 |
Novel thiopeptolide substrates for vertebrate collagenase |
June 24, 1986 |
| The disclosure relates to novel synthetic thiopeptolide substrates having high activity for the enzyme collagenase. These substrates have the following amino acid sequences:whereinR=H or N-protecting group,X=Leu, Ile, Phe, Val, Gln, Ala,Y=Leu, Ile, Phe, Val, Ala,Z=Leu, Ile, Phe, Val, Gln, Al |
| 4569907 |
Thiopeptolide substrates for vertebrate collagenase |
February 11, 1986 |
| The disclosure relates to novel synthetic thiopeptolide substrates having high activity for the enzyme collagenase. These substrates have the following amino acid sequences:whereinR=H or N-protecting group,X=Leu, Ile, Phe, Val, Gln, Ala,Y=Leu, Ile, Phe, Val, Ala,Z=Leu, Ile, Phe, Val, Gln, Al |
| 4537860 |
Static cell culture maintenance system |
August 27, 1985 |
| A method and apparatus for maintaining animal cells in vitro in a substantially arrested state of proliferation with continuous secretion of cell product is disclosed. The cells are retained within a reactor vessel chamber in a semi-rigid matrix having interstices for passage of fluid |
| 4335215 |
Method of growing anchorage-dependent cells |
June 15, 1982 |
| Anchorage-dependent cells are grown in agitated microcarrier suspension in which the cells and microcarriers are aggregated by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment w |
| 4289854 |
Cell culture method and apparatus |
September 15, 1981 |
| A cell culture system and apparatus is provided for the agitated suspension culturing of mammalian cells in a flask assembly in which the culture medium and cells are gently agitated by the slow rotation of agitator means suspended downwardly in the culture medium and having at least one |
| 4273871 |
Production of angiogenic factor by cell culture |
June 16, 1981 |
| Process for the production of human angiogenic factor in vitro comprising growing cells of human foreskin fibroblast cell line on support surface in nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate angiogenic factor and recovering the resulting |
| 4268629 |
Production of angiogenic factor by cell culture |
May 19, 1981 |
| Process for the production of human angiogenic factor in vitro comprising growing the human diploid cell line IMR-90 on support surface in nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate angiogenic factor and recovering the resulting angiogeni |
| 4253684 |
Quick connect coupler with air shield |
March 3, 1981 |
| A quick connect coupler for fluid transfer is disclosed in which one or both of the mating coupler members is provided with a sleeve which encloses at least a portion of said coupler member adjacent to its coupling end. The sleeve has an inlet for admission of air through its outer w |
| 4229532 |
Production of tumor angiogenesis factor by cell culture |
October 21, 1980 |
| Process for the production of human TAF in vitro comprising growing the human osteosarcoma cell line MG-63 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or |
| 4229531 |
Production of tumor angiogenesis factor by cell culture |
October 21, 1980 |
| Process for the production of human TAF in vitro comprising growing the human colon adenocarcinoma cell line HT-29 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the |
| 4225670 |
Production of tumor angiogenesis factor by cell culture |
September 30, 1980 |
| Process for the production of human TAF in vitro comprising growing the human Burkitt lymphoma cell line Raji in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells |
| 4217412 |
Production of tumor angiogenesis factor by cell culture |
August 12, 1980 |
| Process for the production of human TAF in vitro comprising growing the human medulloblastoma cell line TE-671 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the |
| 4210719 |
Production of tumor angiogenesis factor by cell culture |
July 1, 1980 |
| Process for the production of human TAF in vitro comprising growing the human rhabdomyosarcoma cell line RD in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells o |
| 4210718 |
Production of tumor angiogenesis factor by cell culture |
July 1, 1980 |
| Process for the production of human TAF in vitro comprising growing the human melanoma cell line A-375 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cel |
| 4209587 |
Production of tumor angiogenesis factor by cell culture |
June 24, 1980 |
| Process for the production of human TAF in vitro comprising growing the human liver adenocardinoma cell line SK-HEP-1 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF fr |
| 4201845 |
Cell culture reactor |
May 6, 1980 |
| A cell culture reactor is provided for the growth of cells in vitro which employs elongate, selectively permeable hollow fibers arranged in a shallow layer configuration as a matrix for cell attachment on the outer surface of the fibers, and aeration of the cells by passage through the |
| 4190708 |
Production of urokinase |
February 26, 1980 |
| The yield of urokinase produced by cell culture of kidney cells is improved by incorporating in the cell culture medium an elevated level of phenylalanine. |
| 4187149 |
Cell culture sampling system |
February 5, 1980 |
| A cell culture sampling device is provided which comprises a double septum holder having an axial bore therethrough, upper and lower septa retaining members, said upper retaining member having lip means to retain immobilized an external septum and said lower retaining member having s |
| 4184916 |
Continuous cell culture system |
January 22, 1980 |
| A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly |
| 4178209 |
Continuous cell culture method and apparatus |
December 11, 1979 |
| A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without any substantial cell disruption and clogging of the filter with c |
| 4166768 |
Continuous cell culture system |
September 4, 1979 |
| A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly |
| 4087327 |
Mammalion cell culture process |
May 2, 1978 |
| A cell culture reactor system is provided for the growth of cells in vitro which employs elongate hollow or solid fibers arranged in a shallow layer configuration as a matrix for cell attachment in which the flow path of the culture media is directed substantially uniformly through the f |
| 4059485 |
Adaptation of cell lines to suspension culture |
November 22, 1977 |
| Densely packed cells growing rapidly in substrate-attached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 .mu. to 500 .mu. in diameter. These aggregates are then transferred to fresh medium in agitated liquid suspension. The c |
