| Patent Number |
Title Of Patent |
Date Issued |
| 7422902 |
Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate |
September 9, 2008 |
| Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or |
| 7273620 |
Triggered release of liposomal drugs following mixing of cationic and anionic liposomes |
September 25, 2007 |
| Methods and compositions for triggering the delivery of an encapsulated therapeutic agent from a liposome are provided. Liposomes of opposite charge and incorporating lipids which favor non-lamellar structures are contacted in vivo. At least one of the liposome encapsulates at least one |
| 7205273 |
Fusogenic liposomes |
April 17, 2007 |
| The present invention relates to liposomes and virosomes and, more particularly, to liposomal and virosomal delivery systems for transporting materials such as drugs, nucleic acids and proteins. |
| 7189705 |
Methods of enhancing SPLP-mediated transfection using endosomal membrane destabilizers |
March 13, 2007 |
| The present invention provides novel and surprisingly effective methods for delivering nucleic acids to cells. These methods are based upon the discovery that the presence of endosomal membrane destabilizers (e.g., calcium) leads to a dramatic increase in the transfection efficiency of |
| 7094423 |
Methods for preparation of lipid-encapsulated therapeutic agents |
August 22, 2006 |
| Fully lipid-encapsulated therapeutic agent particles of a charged therapeutic agent are prepared by combining a lipid composition containing preformed lipid vesicles, a charged therapeutic agent, and a destabilizing agent to form a mixture of preformed vesicles and therapeutic agent |
| 6852334 |
Cationic peg-lipids and methods of use |
February 8, 2005 |
| The present invention provides cationic-polymer-lipid conjugates (CPLs) such as distal cationic-poly(ethylene glycol)-lipid conjugates which can be incorporated into conventional and stealth liposomes or other lipid-based formulation for enhancing cellular uptake. The CPLs of the pre |
| 6835395 |
Composition containing small multilamellar oligodeoxynucleotide-containing lipid vesicles |
December 28, 2004 |
| Lipidic compositions with superior characteristics for in vivo delivery of oligodeoxynucleotides (ODN) can easily and efficiently be made in the form of small multilamellar vesicles. The compositions contain a population of nucleic acid-containing lipid vesicles in a liquid carrier, and |
| 6815432 |
Methods for encapsulating plasmids in lipid bilayers |
November 9, 2004 |
| Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are |
| 6759057 |
Methods and compositions using liposome-encapsulated non-steroidal anti-inflammatory drugs |
July 6, 2004 |
| Methods and compositions are described for the treatment of inflammatory diseases including the use of liposomes to deliver non-steroidal anti-inflammatory drugs. Drugs may be encapsulated in the liposomes during their preparation, or alternatively, are combined with the liposomes fo |
| 6734171 |
Methods for encapsulating nucleic acids in lipid bilayers |
May 11, 2004 |
| The present invention relates to lipid-based formulations for nucleic acid delivery to cells, methods for the preparation of such formulations and, in particular, to lipid encapsulated plasmids. The compositions are safe and practical for clinical use. In addition, the present invention |
| 6673364 |
Liposome having an exchangeable component |
January 6, 2004 |
| The present invention provides a fusogenic liposome comprising a lipid capable of adopting a non-lamellar phase, yet capable of assuming a bilayer structure in the presence of a bilayer stabilizing component; and a bilayer stabilizing component reversibly associated with the lipid to |
| 6586410 |
Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate |
July 1, 2003 |
| Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or |
| 6534484 |
Methods for encapsulating plasmids in lipid bilayers |
March 18, 2003 |
| Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are |
| 6417326 |
Fusogenic liposomes |
July 9, 2002 |
| The present invention relates to liposomes and virosomes and, more particularly, to liposomal and virosomal delivery systems for transporting materials such as drugs, nucleic acids and proteins. |
| 6406713 |
Methods of preparing low-toxicity drug-lipid complexes |
June 18, 2002 |
| Methods and compositions are described for nonliposomal lipid complexes in association with toxic hydrophobic drugs such as the polyene antibiotic amphotericin B. Lipid compositions are preferably a combination of the phospholipids dimyristoylphosphatidylcholine (DMPC) and dimyristoy |
| 6083530 |
High drug:lipid formulations of liposomal-antineoplastic agents |
July 4, 2000 |
| A method for encapsulation of antineoplastic agents in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes may be made by a process that loads the drug by an active mechanism using a transmembrane ion gradient, preferably a transmembrane pH gradient. Using thi |
| 5981501 |
Methods for encapsulating plasmids in lipid bilayers |
November 9, 1999 |
| Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are |
| 5976567 |
Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate |
November 2, 1999 |
| Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or |
| 5922350 |
Methods of dehydrating, storing and rehydrating liposomes |
July 13, 1999 |
| Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome |
| 5885613 |
Bilayer stabilizing components and their use in forming programmable fusogenic liposomes |
March 23, 1999 |
| The present invention provides a fusogenic liposome comprising a lipid capable of adopting a non-lamellar phase, yet capable of assuming a bilayer structure in the presence of a bilayer stabilizing component; and a bilayer stabilizing component reversibly associated with the lipid to |
| 5837282 |
Ionophore-mediated liposome loading |
November 17, 1998 |
| Novel methods are provided for loading a weakly basic drug into liposomes utilizing an electoneutral transport system. In these methods, ionophores are utilized with liposomes having a metal ion gradient to facilitate the exchange of metal ions for protons. The transported metal ion will |
| 5837279 |
Encapsulation of ionizable agents in liposomes |
November 17, 1998 |
| Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome |
| 5800833 |
Method for loading lipid vesicles |
September 1, 1998 |
| Methods for the preparation of stable liposome formulations of protonatable therapeutic agents. The methods involve loading a therapeutic agent into preformed liposomes having a methylamine concentration gradient across the lipid bilayer of the liposomes. These methods provide liposome |
| 5795589 |
Liposomal antineoplastic agent compositions |
August 18, 1998 |
| A method for encapsulation of antineoplastic agents in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes may be made by a process that loads the drug by an active mechanism using a transmembrane ion gradient, preferably a tranamembrane pH gradient. Using thi |
| 5785987 |
Method for loading lipid vesicles |
July 28, 1998 |
| Methods for the preparation of stable liposome formulations of protonatable therapeutic agents. The methods involve loading a therapeutic agent into preformed liposomes having a methylamine concentration gradient across the lipid bilayer of the liposomes. These methods provide liposome |
| 5780054 |
Methods for increasing the circulation half-life of protein-based therapeutics |
July 14, 1998 |
| Methods of increasing the circulation half-life of protein-based therapeutics in a host, the methods comprising: (a) administering to the host an amount of a first liposome formulation comprising liposomes and an antineoplastic agent; and (b) administering to the host a second formul |
| 5744158 |
Methods of treatment using high drug-lipid formulations of liposomal-antineoplastic agents |
April 28, 1998 |
| A method for encapsulation of antineoplastic agents in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes may be made by a process that loads the drug by an active mechanism using a transmembrane ion gradient, preferably a transmembrane pH gradient. Using thi |
| 5736155 |
Encapsulation of antineoplastic agents in liposomes |
April 7, 1998 |
| Methods for encapsulating ionizable antineoplastic agents in liposomes using transmembrane potentials are provided. Trapping efficiencies approaching 100% and rapid loading are readily achieved. Dehydration protocols which allow liposomes to be conveniently used in the administration |
| 5616341 |
High drug:lipid formulations of liposomal antineoplastic agents |
April 1, 1997 |
| A method for encapsulation of antineoplastic agents in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes may be made by a process that loads the drug by an active mechanism using a transmembrane ion gradient, preferably a transmembrane pH gradient. Using thi |
| 5616334 |
Low toxicity drug-lipid systems |
April 1, 1997 |
| Methods and compositions are described for nonliposomal lipid complexes in association with toxic hydrophobic drugs such as the polyene antibiotic amphotericin B. Lipid compositions are preferably a combination of the phospholipids dimyristoylphosphatidylcholine (DMPC) and dimyristoy |
| 5595756 |
Liposomal compositions for enhanced retention of bioactive agents |
January 21, 1997 |
| Liposomal compositions encapsulating bioactive agents and having improved circulation longevity of the agents are disclosed. Such liposomes combine a low pH of the solution in which a bioactive agent is entrapped and a sugar-modified lipid or an amine-bearing lipid, the combination of wh |
| 5578320 |
Method of dehydrating liposomes using protective sugars |
November 26, 1996 |
| Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome |
| 5552155 |
Fusogenic lipsomes and methods for making and using same |
September 3, 1996 |
| A liposome composition is provided which contains a liposome having: (i) an outermost lipid bilayer containing, in addition to a neutral, bilayer preferring lipid, a fusion-promoting effective amount of an ionizable lipid having a protonatable, cationic headgroup and an unsaturated acyl |
| 5525232 |
Method for entrapment of cationic species in lemellar vesicles |
June 11, 1996 |
| Methods are disclosed for entrapment of a cation in a vesicle having a membrane and an acidic aqueous compartment comprising contacting the vesicle with a buffer solution comprising the cation and a lipophilic, carboxylic ionophoretic antibiotic capable of complexing with the cation |
| 5409704 |
Liposomes comprising aminoglycoside phosphates and methods of production and use |
April 25, 1995 |
| Aminoglycosides, analogs and derivatives thereof, in the form of phosphate salts are described as well as the process for making and utilizing same. Aminoglycoside phosphate liposomes and nonguanadino aminoglycoside phosphate liposomes, their preparation and use, are particularly des |
| 5399331 |
Method for protein-liposome coupling |
March 21, 1995 |
| This invention relates to a method for synthesizing a substantially pure reactive lipid including, for example, N-[4-(p-maleimidophenyl)-butyryl]phosphatidylethanolamine (MPB-PE) and related compositions. The compositions of the present invention are useful as coupling agents and may |
| 5380531 |
Accumulations of amino acids and peptides into liposomes |
January 10, 1995 |
| The present invention relates to liposomal compositions having a concentration gradient which load amino acids and peptides exhibiting weak acid or base characteristics into liposomes. Specifically loaded into liposomes by the methods of the present invention are C-terminal substitut |
| 5376452 |
Induction of asymmetry in vesicles |
December 27, 1994 |
| Methods are described for controlling the transbilayer distribution of ionizable lipids and proteins in vesicles. Control of the ion gradient of the exterior bathing medium in relation to that of the interior entrapped aqueous compartment of the vesicles induces migration of ionizable li |
| 5252263 |
Induction of asymmetry in vesicles |
October 12, 1993 |
| Methods are described for controlling the transbilayer distribution of ionizable lipids and proteins in vesicles. Control of the ion gradient of the exterior bathing medium in relation to that of the interior entrapped aqueous compartment of the vesicles induces migration of ionizable li |
| 5204112 |
Induction of asymmetry in vesicles |
April 20, 1993 |
| Methods are described for controlling the transbilayer distribution of ionizable lipids and proteins in vesicles. Control of the ion gradient of the exterior bathing medium in relation to that of the interior entrapped aqueous compartment of the vesicles induces migration of ionizable li |
| 5171578 |
Composition for targeting, storing and loading of liposomes |
December 15, 1992 |
| The present invention describes a composition consisting of liposomes covalently or non-covalently coupled to the glycoprotein streptavidin. The streptavidin may additionally be coupled to biotinated proteins such as Immunoglobulin G or monoclonal antibodies.The liposomes of the inventio |
| 5077056 |
Encapsulation of antineoplastic agents in liposomes |
December 31, 1991 |
| Methods for encapsulating ionizable antineoplastic agents in liposomes using transmembrane potentials are provided. Trapping efficiencies approaching 100% and rapid loading are readily achieved. Dehydration protocols which allow liposomes to be conveniently used in the administration |
| 5059421 |
Preparation of targeted liposome systems of a defined size distribution |
October 22, 1991 |
| The present invention relates to a general method for producing sized protein-liposome conjugates exhibiting enhanced blood circulation times. The present invention also relates to the sized protein-liposome conjugate compositions produced by the method of the present invention. The |
| 5008050 |
Extrusion technique for producing unilamellar vesicles |
April 16, 1991 |
| A method for reducing the lamellarity of a population of liposomes is provided which comprises repeatedly passing the liposomes under pressure through a filter which has a pore size equal to or less than about 100 nm. In certain embodiments, the method is used to convert a population of |
| 4975282 |
Multilamellar liposomes having improved trapping efficiencies |
December 4, 1990 |
| A multilamellar vesicle dispersed in an aqueous phase comprising an aqueous medium, a lipid concentration of at least about 50 mg/ml and a trapping efficiency of at least about 40 percent. The vesicle can be prepared by dispersing the lipid in an aqueous phase to form a multilamellar ves |
| 4923854 |
Solubilization of hydrophobic materials using lysophospholipid |
May 8, 1990 |
| A method and composition are described for the solubilization of hydrophobic materials using a lysophospholipid. The method includes drying a composition comprising a hydrophobic material-solubilizing effective amount of phospholipid from organic solvent and hydrating the resulting f |
| 4885172 |
Composition for targeting, storing and loading of liposomes |
December 5, 1989 |
| The present invention describes a composition consisting of liposomes covalently or non-covalently coupled to the glycoprotein streptavidin. The streptavidin may additionally be coupled to biotinated proteins such as Immunoglobulin G or monoclonal antibodies.The liposomes of the inventio |
| 4880635 |
Dehydrated liposomes |
November 14, 1989 |
| Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome |
