| Patent Number |
Title Of Patent |
Date Issued |
| RE38723 |
Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
April 12, 2005 |
| The subject invention provides substrates useful for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process inv |
| 7226740 |
Aza-benzazolium containing cyanine dyes |
June 5, 2007 |
| Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually |
| 6972326 |
Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes |
December 6, 2005 |
| The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or d |
| 6967251 |
Reagents for labeling biomolecules having aldehyde or ketone moieties |
November 22, 2005 |
| Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: ##STR1##wherein Q is carbonyl, thiocarbonyl, or sulfonyl, |
| 6962992 |
Crown ether derivatives |
November 8, 2005 |
| The invention describes crown ether chelators, including crown ethers having the formula ##STR1##and aza-substituted and thia-substituted analogs thereof. These crown ethers are substituted by a dye moiety, a chemically reactive group, a conjugated substance, or a combination thereof |
| 6716979 |
Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings |
April 6, 2004 |
| The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the |
| 6664047 |
Aza-benzazolium containing cyanine dyes |
December 16, 2003 |
| Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually |
| 6579718 |
Carbazolylvinyl dye protein stains |
June 17, 2003 |
| The present invention describes a variety of substituted and unsubstituted carbazolylvinyl dyes and their use for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by |
| 6562632 |
Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
May 13, 2003 |
| The invention describes reactive dyes having an alkyl spacer attached via a sulfonamide bond to a sulforhodamine 101 fluorophore, and a variety of useful conjugates prepared therefrom. The increased length of the covalent linkage due to the alkyl spacer results in dye-conjugates having a |
| 6399392 |
Xanthene dyes and their application as luminescence quenching compounds |
June 4, 2002 |
| The quenching compounds of the invention are nitrogen-substituted xanthenes that are substituted by one or more aromatic or heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable fluorescence and efficiently quench a broad spectrum of |
| 6323337 |
Quenching oligonucleotides |
November 27, 2001 |
| The invention relates to oligonucleotides labeled with an energy transfer acceptor useful in conjunction with fluorescent nucleic acid stains. The resulting oligonucleotides are useful for decreasing background fluorescence during amplification assays and in ligation assays, and for |
| 6265179 |
Detection of phosphate using coupled enzymatic reactions |
July 24, 2001 |
| Inorganic phosphate may be detected and optionally quantified via the coupling of a phosphate-dependent enzymatic reaction with an enzyme system that generates hydrogen peroxide in the presence of a chromogenic or fluorogenic peroxidase substrate. Phosphate consuming or phosphate-pro |
| 6162931 |
Fluorinated xanthene derivatives |
December 19, 2000 |
| The family of dyes of the invention are fluoresceins and rhodols that are directly substituted on one or more aromatic carbons by fluorine. These fluorine-substituted fluorescent dyes possess greater photostability and have lower sensitivity to pH changes in the physiological range of 6- |
| 6130101 |
Sulfonated xanthene derivatives |
October 10, 2000 |
| The present invention describes xanthene dyes, including rhodamines, rhodols and fluoresceins that are substituted one or more times by a sulfonic acid or a salt of a sulfonic acid. The dyes of the invention, including chemically reactive dyes and dye-conjugates are useful as fluores |
| 6013802 |
Fluorescent conjugates of metal-chelating nitrogen heterocycles |
January 11, 2000 |
| The present invention describes a family of fluorescent indicators for metal cations. The indicators are fluorophore conjugates of pyridyl-based metal ion chelators. The indicators are very sensitive detection as quantification reagents for a variety of metals, in a variety of oxidation |
| 6004536 |
Lipophilic cyanine dyes with enchanced aqueous solubilty |
December 21, 1999 |
| The present invention relates to a family of cyanine dyes possessing lipophilic alkyl chains and either one or more reactive functional groups, bromo or chloro, or phenyl, sulfophenyl or polysulfophenyl substituents or combinations thereof. The dyes of the invention are useful for staini |
| 5872243 |
Caged nucleotides |
February 16, 1999 |
| The present invention describes a family of photolabile caged nucleotides, including cyclic nucleotides. The compounds of the present invention are caged analogs and derivatives of NAD.sup.+, NADH, NADP, NADPH, NAADP and cADPR. The photolysis of the present compounds allows the release o |
| 5863753 |
Chemically reactive unsymmetrical cyanine dyes and their conjugates |
January 26, 1999 |
| The invention comprises cyanine dyes, in particular chemically reactive dyes, conjugates of reactive cyanine dyes, the non-covalent complexes of nucleic acids with the dyes and dye-conjugates of the invention, and a method of forming a nucleic acid complex with the dyes and dye-conjugate |
| 5830912 |
Derivatives of 6,8-difluoro-7-hydroxycoumarin |
November 3, 1998 |
| The present invention comprises 6,8-difluoro-7-hydroxycoumarins and derivatives of 6,8-difluoro-7-hydroxycoumarins, including reactive dyes, dye-conjugates and enzyme substrates. These fluorine-substituted fluorescent dyes typically possess greater photostability and lower pH sensiti |
| 5798276 |
Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
August 25, 1998 |
| The invention describes reactive dyes having an alkyl spacer attached via a sulfonamide bond to a sulforhodamine 101 fluorophore, and a variety of useful conjugates prepared therefrom. The increased length of the covalent linkage due to the alkyl spacer results in dye-conjugates having a |
| 5786219 |
Microspheres with fluorescent spherical zones |
July 28, 1998 |
| The invention describes novel fluorescently labeled microspheres, where the microspheres possess at least one internal fluorescent spherical zone. The invention also describes the method of preparing the novel microspheres, the method of calibrating microscopy instrumentation using the n |
| 5773236 |
Assay for glutathiane transferase using polyhaloaryl-substituted reporter molecules |
June 30, 1998 |
| The subject invention describes compounds containing a polyhalogenated aryl moiety. The compounds of the invention are particularly useful for the assay of a variety of enzymes, including intracellular enzymes. The subject invention also describes assays for glutathione and/or glutathion |
| 5723218 |
Dipyrrometheneboron difluoride labeled flourescent microparticles |
March 3, 1998 |
| The invention is a novel fluorescently labeled microparticle, where the microparticle internally incorporates at least one dipyrrometheneboron difluoride dye. Appropriate selection of substituents results in dipyrrometheneboron difluoride derivatives that, when incorporated into poly |
| 5719031 |
Dye labeled polymers as reagents for measuring polymer degradation |
February 17, 1998 |
| This invention relates to polymers labeled with fluorescent dye to the point that significant fluorescence quenching occurs, such that degradation of the polymer results in fluorescence enhancement. The resulting fluorescence enhancement is useful for measuring the degradation of suc |
| 5686261 |
Xanthylium dyes that are well retained in mitochondria |
November 11, 1997 |
| The present invention describes a method of staining mitochondria, and analyzing mitochondrial function, using a class of fluorescent substituted 3',6'-diaminoxanthenes and their reduced analogs 3',6'-diaminodihydroxanthenes, which are oxidized to the fluorescent form of the dye in s |
| 5658751 |
Substituted unsymmetrical cyanine dyes with selected permeability |
August 19, 1997 |
| The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a pyridinium or quinolinium ring system having at least one substituent on th |
| 5648270 |
Methods of sensing with fluorescent conjugates of metal-chelating nitrogen heterocycles |
July 15, 1997 |
| The present invention describes the use of a family of fluorescent indicators for metal cations. The indicators are fluorophore conjugates of pyridyl-based metal ion chelators. The indicators are very sensitive detection as quantification reagents for a variety of metals, in a variety |
| 5635608 |
.alpha.-carboxy caged compounds |
June 3, 1997 |
| The invention relates to caged compounds for the study of biological processes, where the caged compound has a photoremoveable .alpha.-carboxy-substituted o-nitrobenzyl group. Covalent attachment of the substituted o-nitrobenzyl to a parent compound yields a caged compound with biolo |
| 5616502 |
Non-specific protein staining using merocyanine dyes |
April 1, 1997 |
| The present invention describes the use of a variety of merocyanine dyes and substituted merocyanine dyes for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by their |
| 5582977 |
Dimers of unsymmetrical cyanine dyes |
December 10, 1996 |
| The invention relates to dimers of unsymmetrical cyanine dyes, typically dimers of benzthiazole or benzoxazole derivatives, that exhibit enhanced fluorescence on binding with DNA or RNA, The dimers generally have the formula: ##STR1## where R.sup.1 and R.sup.2, which may be the s |
| 5576424 |
Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
November 19, 1996 |
| The subject invention provides substrates useful for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process inv |
| 5573909 |
Fluorescent labeling using microparticles with controllable stokes shift |
November 12, 1996 |
| The invention relates to methods for labeling or detecting one or more target materials using surface coated fluorescent microparticles with unique characteristics. The unique microparticles used to practice the invention have at least two components: an external substance or coating |
| 5545535 |
Fluorescent assay for bacterial gram reaction |
August 13, 1996 |
| The invention relates to a method of analyzing a sample thought to contain bacteria using an aqueous solution comprising one or more fluorescent dyes: a fluorescent dye of formula I, a fluorescent dye of formula II, a fluorescent dye of formula III, and a fluorescent dye of formula IV. E |
| 5534416 |
Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
July 9, 1996 |
| The invention relates to a method of analyzing the viability of a sample of cells using an aqueous solution comprising two fluorescent dyes. Dye I has the formula: ##STR1## where R.sup.2 is C.sub.1-6 alkyl; Z.sup.- is a biologically compatible counterion;X is O; S; Se; or NR.sup. |
| 5516864 |
Fluorescent ion-selective diaryldiaza crown ether conjugates |
May 14, 1996 |
| This invention describes novel sensors for ions that are based on the combination of xanthylium-based dyes with metal-binding N,N'-diaryldiaza crown ethers. These sensors are primarily useful for detection and quantitation of alkali-metal ions in aqueous solution. Binding of the ion |
| 5514710 |
Photocleavable derivatives of hydroxyprenesulfonic acids |
May 7, 1996 |
| The invention describes the synthesis and use of photoactivated (or caged) fluorescent dyes. Upon illumination at less than about 400 nm the caged dyes release highly fluorescent, water soluble hydroxypyrenesulfonic acid dyes according to the following equation: ##STR1## X, Y, an |
| 5501980 |
Benzazolylcoumarin-based ion indicators |
March 26, 1996 |
| The indicator compounds of the present invention are substituted or unsubstituted BAPTA-type chelators that contain a benzazolyl-coumarin substructure, and the pharmaceutically acceptable non-toxic salts and esters thereof. These compounds are useful for the detection and quantificat |
| 5459276 |
Benzazolylcoumarin-based ion indicators for heavy metals |
October 17, 1995 |
| The indicator compounds of the present invention are substituted or unsubstituted 5'-nitro-BAPTA chelators that contain a benzazolyl-coumarin substructure, and the pharmaceutically acceptable non-toxic salts and esters thereof. These compounds are useful for the detection and quantif |
| 5459268 |
Xanthylium dyes that are well retained in mitochondria |
October 17, 1995 |
| The dyes of the present invention are fluorescent substituted 3',6'-diaminoxanthenes and their reduced analogs 3',6'-diaminodihydroxanthenes, which are oxidized to the fluorescent form of the dye in situ. In their oxidized form, the dyes selectively localize within mitochondria. The |
| 5453517 |
Reactive derivatives of bapta used to make ion-selective chelators |
September 26, 1995 |
| The invention relates to fluorescent and/or reactive derivatives of 1,2-bis-(2-aminophenoxyethane)-N,N,N',N'-tetraacetic acid (BAPTA) according to the formula: ##STR1## where at least one of W and X is a functional group, with or without a spacer, that terminates in an alcohol or |
| 5451663 |
Long wavelength chemically reactive dipyrrometheneboron difluoride dyes and conjugates |
September 19, 1995 |
| This invention relates to derivatives of dipyrrometheneboron difluoride fluorescent dyes that have an absorption maximum at wavelengths longer than about 525 nm, and are chemically reactive with nucleic acids, proteins, carbohydrates, and other biologically derived or synthetic chemi |
| 5445946 |
Intravacuolar stains for yeast and other fungi |
August 29, 1995 |
| The invention relates to a method of forming distinctive intravacuolar structures that are unique to fungal cells and can be correlated with cell viability. The preferred dyes of the present invention are described by the formula: ##STR1## wherein each R.sup.1 is independently H; |
| 5442045 |
Biological conjugates of fluorescent rhodol dyes |
August 15, 1995 |
| Novel fluorescent dyes based on the rhodol structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found in biomolecules or polymers including amines, phenols, thiols, acids, aldehydes and ketones |
| 5437980 |
Phenanthridium dye staining of nucleic acids in living cells |
August 1, 1995 |
| The invention relates to use of fluorescent compounds of the formula: ##STR1## where R contains between 4 and about 10 carbons and is optionally saturated or unsaturated, and is linear or branched or contains an alicyclic or aromatic ring; and the symbol .PSI. depicts the presence of |
| 5436134 |
Cyclic-substituted unsymmetrical cyanine dyes |
July 25, 1995 |
| The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. In particular, the invention describes unsymmetrical cyanine dyes having a saturated or unsaturated cyclic substituent. The dyes of the invention possess super |
| 5433896 |
Dibenzopyrrometheneboron difluoride dyes |
July 18, 1995 |
| The invention relates to fluorescent dyes that are substituted or unsubstituted derivatives of 1-[isoindolyl]methylene-isoindole that are bound through both isoindole nitrogens to a boron difluoride moiety, forming a fluorescent dibenzopyrrometheneboron difluoride compound ##STR1## |
| 5410030 |
Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
April 25, 1995 |
| The invention relates to nucleic acid stains that are homo- or heterodimers of unsymmetrical cyanine dyes, where at least one dye unit of the dimer is a substituted or unsubstituted benzazolium- or naphthazolium-pyridinium derivative, which dye unit is linked to another unsymmetrical cya |
| 5405975 |
Fluorescent ion-selective diaryldiaza crown ether conjugates |
April 11, 1995 |
| This invention describes novel sensors for ions that are based on the combination of xanthylium-based dyes with metal-binding N,N'-diaryldiaza crown ethers. These sensors are primarily useful for detection and quantitation of alkali-metal ions in aqueous solution. Binding of the ion |
| 5364764 |
Fluorescent chloramphenicol derivatives for determination of chloramphenicol acetyltransferase a |
November 15, 1994 |
| Fluorescent compounds useful in the determination of chloramphenicol acetyltransferase (CAT) enzyme activity are described. The compounds are "fluorescent derivatives related in structure to chloramphenicol and are acylated in the presence of CAT to produce fluorescent mono- and diac |
| 5362628 |
Fluorescent haloalkyl derivatives of reporter molecules well retained in cells |
November 8, 1994 |
| The subject invention provides a method for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process involving co |
