Resources Contact Us Home
Methods and algorithms for cell enumeration in a low-cost cytometer

Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

  Recently Added Patents
System and methods for random parameter filtering
Method and apparatus for supporting delivery, sale and billing of perishable and time-sensitive goods such as newspapers, periodicals and direct marketing and promotional materials
Retransmission and retransmission request in data communication systems
3D solid-state arrangement for solid state memory
Reference circuit with curvature correction using additional complementary to temperature component
System, method and program product for guiding correction of semantic errors in code using collaboration records
Wristband with detachable labels
  Randomly Featured Patents
Insulating tubular conduit apparatus and method
Chime-engaging package for containers
Apparatus and process for cleaning the outside of containers
Method of forming a shallow trench isolation structure
Heat-sensitive recording material
Portable body support apparatus for automotive mechanics
Method for resonant wave mixing in closed containers
Idle regulating valve control system for engine
Medical emergency treatment cart
Printers with simplex and duplex cut sheet fusing