Resources Contact Us Home
Methods and algorithms for cell enumeration in a low-cost cytometer

Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

  Recently Added Patents
Method and system for shared high speed cache in SAS switches
Information processing apparatus, method for controlling information processing apparatus, and storage medium
Sharing networks determined by sharing of items between individuals
Vanilloid receptor ligands and use thereof for the production of pharmaceutical preparations
Scanning optical positioning system with spatially triangulating receivers
Headset electronics
Method of adenoviral vector synthesis
  Randomly Featured Patents
Processes for the preparation of derivatives of 4a, 5, 9, 10, 11, 12-hexahydro-6H-benzofuro-[3a, 3, 2-ef][2]benzazepine
Lead-palladium battery
Image recording apparatus having pivotable roller arm
Optical fiber coupling unit and optical waveguide arrangement, and method of producing an optical fiber coupling unit
Solar thermal system for the partial desalination of seawater and electrical energy
Low temperature packaging apparatus and method
Immunoconjugates joined by thioether bonds having reduced toxicity and improved selectivity
Apparatus and method for picking and feeding print media sheets