Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Methods and algorithms for cell enumeration in a low-cost cytometer










Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.








 
 
  Recently Added Patents
Control method of LPI lamp for LPI vehicle and logic therefor
System and method for broadcasting rich media to devices over multiple carriers
Clip
Screen
Analog-to-digital converter with input voltage biasing DC level of resonant oscillator
Power-on reset circuit
Integrated control system for stability control of yaw, roll and lateral motion of a driving vehicle using an integrated sensing system to determine longitudinal velocity
  Randomly Featured Patents
Image forming apparatus
Retail system for selling products based on a flexible product description
Optical inspection apparatus for substrate defect detection
Picture window frame
Autoloading apparatus for photographic film
Jackknife warning system for trailered vehicle
Loading device for the at least partially automated loading and unloading of a cargo hold on transport equipment as well as a conveying system
Method for cleaning structural surface
Digital phase measurement system
Blade drive device