Resources Contact Us Home
Methods and algorithms for cell enumeration in a low-cost cytometer

Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

  Recently Added Patents
Simplified algorithm for abnormal situation prevention in load following applications including plugged line diagnostics in a dynamic process
Pyrrolidine-1,2-dicarboxamide derivatives
Statistical identification of instances during reconciliation process
Method for releasing a locking in mobile terminal and mobile terminal using the same
Elementary network description for efficient link between neuronal models and neuromorphic systems
Tomlinson Harashima precoding with additional receiver processing in a multi-user multiple-input multiple-output wireless transmission system
Feedback method and processing system for policy installation failures
  Randomly Featured Patents
One layer spider interconnect
Integrated CMOS gate-array circuit
Mobile container
Adhesive material
Wheel apparatus for an irrigation system
Defect inspecting device for sample surface and defect detection method therefor
Stylus pen
FinFET alignment structures using a double trench flow
Temperature stabilized constant current source suitable for charging charge depleted battery with single power supply
Method of arranging the staggered shape bond pads layers for effectively reducing the size of a die