Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Methods and algorithms for cell enumeration in a low-cost cytometer










Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.








 
 
  Recently Added Patents
Electronic apparatus and manufacturing method of electronic apparatus
Method and apparatus for blind signal extraction
Light modulators and optical apparatuses including the same
Touch sensing technology
Multi display device and method of controlling the same
Method for dynamic multi-level pricing for wireless communications according to quality of service
Polymer compositions and nonwoven compositions prepared therefrom
  Randomly Featured Patents
Portable armrest
Lighting control keypad
Device and method for supporting a disk
Belt and buckle
Small volume in vitro analyte sensor and methods of making
On the fly configuration of electronic device with attachable sub-modules
Decorative window candle top
Sliding tandem media feeder in a printer
Secure content objects
Motor oil performance-enhancing formulation