Resources Contact Us Home
Methods and algorithms for cell enumeration in a low-cost cytometer

Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

  Recently Added Patents
Generating and modifying textual code interfaces from graphical programs
Micromachined devices and fabricating the same
Cardiopulmonary resuscitation monitoring apparatus
Image correction method
Process for production of nickel oxide-stabilized zirconia composite oxide
Assay for the diagnosis of flaviviral infection using antibodies with high affinity for NS1 protein of flavivirusi in hexameric form
Treatment of celiac disease with IgA
  Randomly Featured Patents
Carpenter bee traps
Child's training pant with elasticized shaped absorbent and method of making the same
Constant values in mixed language programming environments
Water control valve core with a segmented locking function
Circuits for sensing current levels within lighting apparatus
Apparatus and method for synchronizing base sites individually in a communication system
Chemical mechanical polishing apparatus
Extrusion head for extruding mouldings for roller blinds, frames, finish and the like
Magnetic tape cassette storage case