Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Methods and algorithms for cell enumeration in a low-cost cytometer










Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.








 
 
  Recently Added Patents
Feature management of a communication device
Method and device for evaluating evolution of tumoural lesions
Morphinan compounds
Method for reserving network bandwidth for versioned network services
Location-type tagging using collected traveler data
Systems and methods for dynamically modifying subcriber service profile stored in home location register while roaming in wireless telecommunication networks
Cross-platform cloud-based map creation
  Randomly Featured Patents
Controlled-release salt sensitive capsule for oral use and adhesive system
Variable buoyancy setting flapper
Sample and hold circuit and method therefor
Apparatus for arranging a lamp on a reflector of a vehicle headlight
Intrusion detection switch housing
Galley waste disposal system and method
Method of evaluating deteriorated state of exhaust gas adsorbent
Method of utilizing adjustable height bladder securement mechanism to effect advantageous bladder movement during curing process
Information additive code generator and decoder for communication systems
System security approaches using multiple processing units