Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Methods and algorithms for cell enumeration in a low-cost cytometer










Image Number 12 for United States Patent #8128890.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.








 
 
  Recently Added Patents
Server providing pseudo print preview and final regular preview to device
Cosmetic/dermatological compositions comprising naphthoic acid compounds and polyurethane polymers
Decontamination apparatus and method
System for determining potential lot consolidation during manufacturing
Antibodies to CCR2
Systems and methods for vehicle cruise control
State detection device, electronic apparatus, measurement system and program
  Randomly Featured Patents
Modular refrigeration system
Method and apparatus for spreading and distributing a solid or liquid particulate material
Two-loop, disposable, plastic law enforcement restraint
Treatment of contaminated water
Exposure device having light shading members for correcting light distribution of a projection mechanism in a copier
Weaving loom with articulated suction apparatus for reducing deposition of fly lint and dust
High molecular weight polyimidoylamidine and a polytriazine derived therefrom
Photosensitive drum with improved shutter structure and image forming apparatus having the same
Party display assembly
Apparatus and methods for feeding sheets of media to a media processor