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Methods for determining protein binding specificity using peptide libraries

Image Number 2 for United States Patent #8119572.

A method for determining protein binding specificity using a screen of a peptide library is provided. The method can be used to determine binding specificity for human NAD.sup.+-dependent deacetylase SIRT1, and to identify the most efficiently deacetylated peptide sequences. The method can be also used to screen a combinatorial H4 histone N-terminal tail peptide library to examine the binding preferences of a .alpha.-phos (S1) H4 antibody toward all known possible H4 histone modification states.

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