Resources Contact Us Home
Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry

Image Number 3 for United States Patent #8119411.

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

  Recently Added Patents
Systems and methods for providing power and data to lighting devices
Methods and system for providing drug pricing information from multiple pharmacy benefit managers (PBMs)
Methods and compositions for inhibiting progression to chronic cardiac failure
Process for production of nickel oxide-stabilized zirconia composite oxide
Method and apparatus for map transmission in wireless communication system
Apparatus and method for controlling a tunable matching network in a wireless network
Systems, methods, and devices for selling transaction instruments via web-based tool
  Randomly Featured Patents
System and method for organizing data
Method and apparatus for forming a yarn web on a conveyor
Method and apparatus for determining a vapor signature based upon frequency
Coextruded apertured film sanitary napkin cover
Weighted guitar strap
Differential sensing with high common mode rejection
Recombinant genetic means for the production of serine protease muteins
Handle for atherectomy device
Artificial bonelike graft and method for producing the same
Magnetic recording device, magnetic recording method and magnetic recording medium for shingle write scheme