Resources Contact Us Home
Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry

Image Number 3 for United States Patent #8119411.

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

  Recently Added Patents
Security arrangements for extended USB protocol stack of a USB host system
Method and apparatus for controlling a multi-node process
Taxi cab key chain
Photovoltaic system with integrated photovoltaic panel and battery
System and method for detecting an earth ground fault of an external power supply connected to a vehicle
Collating device, collating method, and program
Reading apparatus and reading method
  Randomly Featured Patents
Flame retardant epoxy resin compositions
Automobile steering wheel and manufacturing method thereof
Dyeing method
Light source with secondary emitter conversion element
Method for treatment of shock
Quiet key switch
Jet engine thrust reverser
Method and apparatus for bifurcating signal transmission over in-phase and quadrature phase spread spectrum communication channels
Liquid dispensing hair removal applicator