Resources Contact Us Home
Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry

Image Number 3 for United States Patent #8119411.

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

  Recently Added Patents
Network traffic demotion
Gaze tracking password input method and device utilizing the same
Polymorphic forms of 3-(1-{3-[5-(1-methyl-piperidin-4-ylmethoxy)-pyrimidin-2-yl]-benzyl}-6-oxo- -1,6-dihydro-pyridazin-3-yl)-benzonitrile hydrochloride salt and processes of manufacturing t
Method for remotely measuring fluctuations in the optical index of refraction of a medium
Rotation angle sensor
Apparatus for touch screen avionic device
Memory device, test operation method thereof, and system including the same
  Randomly Featured Patents
Pixel circuit with reduced wiring
Additive comprising porous calcium phosphate for synthetic resins, and synthetic resin composition
Card reader for computerized access control system
Apparatus and method for rewriting data from volatile memory to nonvolatile memory
Wavelength tunable light source
Die surface magnetic field shield
Method for fabricating microlenses and process of single photomask pattern-based photolithography
System, method and apparatus for an instruction driven digital video processor
Method and composition for bleaching laundry
Method of making a polymer blend composition by reactive extrusion