Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry










Image Number 3 for United States Patent #8119411.

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.








 
 
  Recently Added Patents
Automatic fixup of network configuration on system image move
Anti-FGFR3 antibodies and methods using same
Video-recording reservation management apparatus, method, program, and system
Phase noise extraction apparatus and technique
Cancer treatment kits comprising therapeutic antibody conjugates that bind to aminophospholipids
NMR, instrumentation, and flow meter/controller continuously detecting MR signals, from continuously flowing sample material
Method for generating codewords
  Randomly Featured Patents
Programmable physical action during integrated circuit wafer cleanup
Tiller
Growth of textured gallium nitride thin films and nanowires on polycrystalline substrates
Method for controlling storage device controller, storage device controller, and program
Weather strip
Zoom lens system, imaging device and camera
Integrated circuits and methods for processing integrated circuits with embedded features
Data rate adaptive control device for Manchester code decoders
Electric powered vehicle
Circuit breaker