Resources Contact Us Home
Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry

Image Number 3 for United States Patent #8119411.

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

  Recently Added Patents
Social network user data advertising
Case for electronic device
Purine compounds used as CB2 agonists
Plant disease control composition and its use
Integrated projector system
Handheld electronic communication device having sliding display
Categorization of design rule errors
  Randomly Featured Patents
Combined coin holder and vanity mirror for an automobile dash
Radiant tube heating assembly
Hitch lock
Composition for a topical preparation and a process for producing the same
Aqueous inhalation pharmaceutical composition
Storage and retrieval apparatus
Combination arm rest
Method for processing a silver halide color photographic material
Bitmap font data storage within data processing systems
Bolt locks for freight car, trailer truck and air cargo doors