Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry










Image Number 3 for United States Patent #8119411.

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.








 
 
  Recently Added Patents
Solid-state image sensing device and control method of solid-state image sensing device
LED light
Liquid crystal display device
Process for brominating unsaturated organic compounds with removal of quaternary ammonium or quaternary phosphonium monochlorides
Light fixture
Searchlight
Method and system for automatically hiding irrelevant parts of hierarchical structures in computer user interfaces
  Randomly Featured Patents
Dry shaving apparatus
Condition control system for efficient transfer of energy to and from a working fluid
Energy-conserving illumination system
Shelf
Air/fuel ratio control system for automotive vehicle using feedback control
Display screen with user interface
Template layer formation
Recoil absorbing firearm
Spacer unit
Combined garment and safety harness