Resources Contact Us Home
Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry

Image Number 3 for United States Patent #8119411.

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

  Recently Added Patents
MEMS structure and method for making the same
Keyswitch controller
Reliable event broadcaster with multiplexing and bandwidth control functions
Display screen of a mobile terminal or portion thereof with a graphical user interface
Method of fabricating solid electrolytic capacitor and solid electrolytic capacitor
Image forming apparatus, image forming method, and computer readable medium for identifying target-recording element using detection pattern
Evaluation compiler method
  Randomly Featured Patents
Method and apparatus for machine-reading markings on/in transparent marking substrates
Building block
Light bulb or lighting element protector apparatus with rotatably adjustable cage assembly and which provides information
Clip removing device
System and method for descrambling data
Phase locked loop frequency source having reduced load pull
Ultraviolet lamp for use in water purifiers
Fault tolerant modular subsystems for computers
Upper body protector apparatus and method
Hinged connector for flat display panels