Resources Contact Us Home
Method to produce sterile male flowers and partenocarpic fruits by genetic silencing, associated sequences and vectors containing said sequences

Image Number 3 for United States Patent #7994397.

Genes VvPI from Vitis vinifera cv. Cabernet Sauvignon and LePI from Lycopersicon esculentum are described; together with the use of these genes to produce sterile male flowers and seedless or parthenocarpic fruits. Silencing vectors that comprise these sequences or a part thereof are disclosed. The methods of the invention are directed to producing sterile male flowers and parthenocarpic fruits by genetic silencing, and includes: obtaining the codifying sequence of Pistillata (PI)-homologous genes from the target species; analyzing the expression of the sequence obtained in step (a) to test its expression according to the pattern described for Pistillata genes; analyzing the complementation of PI-gene mutant with the PI sequence obtained from the target species, to assess that the obtained sequence fulfills the function of a PI gene; making a genetic silencing construct that comprises a region of the codifying sequence of PI in a plant expression vector; incorporation of the constructed vector into Agrobacterium tumefaciens; transforming target plants with Agrobacterium tumefaciens modified with the silencing vector and selecting said transformed plants; and checking the absence of Agrobacterium contamination and corroborating transgenic plants by transgene amplification.

  Recently Added Patents
Graphical planner
Distributed mobile access point acquisition
Probe for ultrasound diagnostic apparatus
DL control channel structure enhancement
Method and system of channel detecting and reporting, terminal, and management center
Semiconductor device having a plurality of elements on one semiconductor substrate and method of manufacturing the same
Method and apparatus for policy-based network access control with arbitrary network access control frameworks
  Randomly Featured Patents
Aerospace structure having a cast-in-place noncompressible void filler
High efficiency optical feedback modulator and method of operation
Preparation of recombinant factor VIII in a protein free medium
Advanced microcontroller bus architecture (AMBA) system with reduced power consumption and method of driving AMBA system
Optical system for day and night use
Gate and spring hinge therefore
Composite drill collar
Method of making carbide, nitride and boride powders
Remote power communication system and method thereof
Joystick offset controls