Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 9 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Wireless communication power control
Process information structuring support method
Matching engine for comparing data feeds with user profile criteria
Image sensing apparatus and method of controlling the image sensing apparatus
Touch panel structure and manufacturing method thereof
Information processing using batch setting information
Handbag
  Randomly Featured Patents
Game apparatus including a resilient projectile with a plurality of legs
Bovine teat dip
Method of synthesis of morpholino oligomers
Electric motor with reduced axial length
Multicolor in situ hybridization methods for genetic testing
Combined self holding roller and comb
Ink circulation and wash up system for a press
Apparatus and method for providing communication quality information
Belt-configured saw for cutting slots into stone
Method and apparatus for minimizing chroma subcarrier instability caused by a video line scrambling system