Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 9 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Electrode connector and battery module using the same
Brassiere back fastener
Process for recovering ethanol
System for cyber investigation and data management
Enhanced quality-sorting scheduler
Software service notifications based upon software usage, configuration, and deployment topology
Method and apparatus for determining a list of members for a push to talk communications service
  Randomly Featured Patents
Inflatable amusement toy
Epoxy resin powder composition
Conveyor belt for carrying uncooked product slices through a cooking operation
Method of forming shaped articles
Derivatives of 4-(n-azacycloalkyl) anilides as potassium channel modulators
Automatic textile winding apparatus
Bumper beam structure for vehicle
Production of particulate BBB type polymer having an unusually high surface area
Information terminal with controller for stopping voice data
Steerable surgical access devices and methods