Resources Contact Us Home
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells

Image Number 9 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.

  Recently Added Patents
Systems, methods, and apparatus to determine physical location and routing within a field of low power beacons
Domain isolation through virtual network machines
Solid state lighting devices and associated methods of manufacturing
Compact multi-functional scanning apparatus with retractable flatbed scanner
Vehicle control apparatus
Matching engine for comparing data feeds with user profile criteria
Morphinan compounds
  Randomly Featured Patents
Device for dispensing liquids
Structural truss
One-step bleach and coloring composition for hair and method of using same
Solar heat pipe feedback turbogenerator
System and method of dynamic online session caching
Method and system for directly modulating a voltage controlled oscillator for use in frequency/phase modulated systems
Inductance component comprising a permanent magnet greater in sectional area than a magnetic path and disposed in a magnetic gap
Window blind with fabric assembly
Use of alkyl cyclopentanone and phenyl alkanol derivative-containing compositions for repelling blood feeding arthropods and apparatus for determining repellency and attratancy of semiochemica
Power control for cooking appliance with transient operating modes