Resources Contact Us Home
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells

Image Number 9 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.

  Recently Added Patents
Spectral measurement device
Stroboscopic image modulation to reduce the visual blur of an object being viewed by an observer experiencing vibration
Vacuum cleaner
Power semiconductor module
Tint block image generation program and tint block image generation device
Techniques for forming a contact to a buried diffusion layer in a semiconductor memory device
Philanthropy management apparatus, system, and methods of use and doing business
  Randomly Featured Patents
Weighing apparatus and instrument
Device and method for bicompartmental arthroplasty
Optical scanner providing compensation for non-uniform bulb illumination
Device for retaining a boot on a snowshoe
Switchable loud and quiet exhaust apparatus
Adjustable load automatic releasing cleat
System for certifying whether printed material corresponds to original
Method of extrusion blow-molding polyphenylene sulfide articles
Self-adhesive wallcoverings
Lamp tube switch circuit