Resources Contact Us Home
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells

Image Number 6 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.

  Recently Added Patents
Systems and methods for selective text to speech synthesis
Semiconductor device including a clock generating circuit for generating an internal signal having a coarse delay line, a fine delay line and a selector circuit
Large carrying case
Bipolar junction transistor with a self-aligned emitter and base
Inhibitor of casein kinase 1delta and casein kinase 1E
Second order correction circuit and method for bandgap voltage reference
Vertical gate LDMOS device
  Randomly Featured Patents
Swimming goggles
Machine for minispring socket insertion
Rack system for storing, drying, and deodorizing boots
Disturbance-limiting circuit of redundant type for regulation equipment
III-V and II-VI compounds as template materials for growing germanium containing film on silicon
Memory system having low power consumption
Powder drum
Current monitor for field emission displays
Electronic device having improved user interface
Reference buffer technique for high speed switched capacitor circuits