Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 6 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Controller for internal combustion engine
Method for decoding a spatially multiplexed data signal using a maximum likelihood detection
Stability control system with body-force-disturbance heading correction
Image processing apparatus, image registering method, program causing computer to execute image registering method, and recording medium in which program is recorded
Lentiviral gene transfer vectors and their medicinal applications
Encoder that optically detects positional information of a moving body from different optical paths lengths
Light transformation particle and photobioreactor
  Randomly Featured Patents
Laminated ceramic structure
Methods and apparatus for medical photography
Door stop
Magnetoresistive thin film head
Men's underwear with supporting pocket
Underwater curing of epoxy resin and amine-terminated liquid polymer and product thereof
Developer replenishment unit and image formation apparatus
Device for later application of a two-component material on a substrate
Liquid ejection head and manufacturing method thereof
Injection arrangement for a two-stroke engine