Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 6 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
High electron mobility transistor and manufacturing method thereof
Real-image zoom viewfinder and imaging apparatus
ActRIIB proteins and variants and uses therefore relating to utrophin induction for muscular dystrophy therapy
Grounding fitting
Concentration measuring apparatus for hydrogen sulfide in gas flow, and method for determining sulfide ion
Mobile communication device
Integrated circuit testing with power collapsed
  Randomly Featured Patents
4-Substituted-3-hydroxy-3-pyrroline-2,5-dione inhibitors of glycolic acid oxidase
Plants and seeds of hybrid corn variety CH071844
Device and method for handling objects, in particular items of luggage
Valve and closure assembly for an automatic transmission
Thermoelectric power generating device
Image processing apparatus and method and a computer-readable recording medium on which an image processing program is stored
Medical electrical lead
Printer hammer tip and method for making
Illuminated infant toy
Systems and methods for sensing vector selection in an implantable medical device using a polynomial approach