Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 6 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Automatic detection of image degradation in enhanced vision systems
Business card assembly
Collaborative system for capture and reuse of software application knowledge and a method of realizing same
Mineral, nutritional, cosmetic, pharmaceutical, and agricultural compositions and methods for producing the same
Paper product with surface pattern
Policy based cryptographic application programming interface in secure memory
Image correction method
  Randomly Featured Patents
Magnetic head slider
Light amplifying device
Display device and manufacturing method thereof
Process for producing coproporphyrin III
Line scan sequential lateral solidification of thin films
Method of constructing length-adjustable vehicle steering column assembly
Method for reducing contact resistance
ICBP90 polypeptide and its fragments and polynucleotides coding for said polypeptides and applications for diagnosing and treating cancer
Holder for a food and/or beverage container
Address setting method and communication system employing the address setting method in a multiple ring network utilizing variably set addresses and address write enable/disable states