Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Cooking brush
Label with surface ornamentation
Coordinate measuring device and method for measuring with a coordinate measuring device
Human and mouse targeting peptides identified by phage display
Semiconductor overlapped PN structure and manufacturing method thereof
Synergistic compositions for the treatment of topical viral infections
Proximity-based mobile message delivery
  Randomly Featured Patents
Extended database search
Methods of reducing extravasation of inflammatory cells
Fraud detection based on efficient frequent-behavior sorted lists
Automatic variable linkage mechanism for integrating third party software components
Low latency interconnect bus protocol
Binder composition for powder coatings
System for counteracting a disturbance in a spacecraft
Search light
Motor driving circuit and method
Capacitors with high energy storage density and low ESR