Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Liquid crystal display and manufacturing method thereof
Device and process for isolating and cultivating live cells on a filter or extracting their genetic material
Snap-engagement structure
Systems and methods to provide report part via a web service
Clip
Multi-radio coexistence
Fail-safe upgrading of portable electronic device software
  Randomly Featured Patents
Controlling branch level and viscosity of polyalphaolefins with propene addition
Process for the production of ethers
Pressure testing and refrigerant recharging conduit assembly for automobiles
Tuned torsional vibration damper
Method for diminishing wrinkles and fine lines of the skin by the topical application of .DELTA.5-androstene-3.beta.-ol-7, 17 dione and metabolizable precursors thereof
Magnetic tape transport
Front panel unit for electronic equipment
Method for making multi-layer preform used for plastic blow molding
Spatial harmonic transducers for surface wave devices
Method and apparatus for measuring multi-streams and multi-phase flow