Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Bipolar transistor with diffused layer between deep trench sidewall and collector diffused layer
Systems and methods for managing policies on a computer
Leaky wave mode solar receiver
Memory device, test operation method thereof, and system including the same
Charge pump and method of biasing deep N-well in charge pump
Enterprise seamless mobility
Method and system for dynamically representing distributed information
  Randomly Featured Patents
Baking method of quartz products, computer program and storage medium
Semiconductor integrated circuit device including an ESD protection circuit with an improved ESD protection capability for input or output circuit protection
Rotary cutter for a road planer
Heat exchanger
Transcript apparatus
Method and apparatus for pet tethering device
Injection device with replacement cartridge
Dry shaver with primary and secondary modes of oscillation
Building panel
MEMS actuators with stress releasing design