Resources Contact Us Home
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells

Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.

  Recently Added Patents
Methods for predicting cardiac toxicity
Vibration power generator and vibration power generation device, and communication device and electronic equipment with vibration power generation device
System and method for multi-tiered meta-data caching and distribution in a clustered computer environment
Mobile advertising and compensation-verification system
Image capture system and method
Method and apparatus for controlling peak amplifier and doherty power amplifier
Methods for measuring media performance associated with adjacent track interference
  Randomly Featured Patents
Hitch light system
Digital minimum shift keyed modulator
Method of making pure white light source
Information processing apparatus and method
Super twisted nematic liquid crystal display device having the standard deviation of the spherical grains being not more than 3% and the dispersion quantity of the spherical grains being 100-2
Low I/O bandwidth method and system for implementing detection and identification of scrambling codes
Chrysanthemum plant named `Gedi Three Pyel`
LED current control circuit, current balancer and driving apparatus
Wide-band replica output current sensing circuit
Extension tubes for balloon catheters