Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Managing delivery of application server content
Automatic engine oil life determination with a factor for oil quality
Chair
Method and system for generating and displaying an interactive dynamic selective view of multiply connected objects
Apparatus and method for controlling semiconductor die warpage
Generation of interpolated samples for decision based decoding
Redundant power delivery
  Randomly Featured Patents
Abrasive drill bit
Quality metric-biased region-of-interest coding for video telephony
Reflexive underwriting application responses
Printer with rotated ejection nozzle correction
Gold mining pan
Double current collector negative electrode design for alkali metal ion electrochemical cells
Grille
Internal combustion engine fuel economy improvement system
Spring electrical connectors for a megasonic cleaning system
Characterization of novel gene cbl-SL