Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Pet carrier
Semiconductor memory apparatus
Solid state lighting devices with cellular arrays and associated methods of manufacturing
Over the counter medicinal container with surface ornamentation
Methods and arrangements for realising betting with off-line terminal
Image sensor with improved color crosstalk
Method and apparatus for controlling peak amplifier and doherty power amplifier
  Randomly Featured Patents
Self-leveling tire
Solar cells arrangement
6-(1-fluoroalkyl)-4-pyrimidones and processes for producing the same
Media management interfacing with refined data structures
Multimedia racing experience system
Filter delivery and retrieval device
Information processing apparatus and communication control method
Graphic-arts laser imaging with reduced-length laser cavities and improved performance
Purification of fish oils
Synthesis of 5-azacytidine