Resources Contact Us Home
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells

Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.

  Recently Added Patents
Protective vest
Matching a usage history to a new cloud
Utility knife
Method and system for routing telephony communications together with modified calling party identifier information
Anti-phishing system and method
Geo-coding images
Method to quantify siRNAs, miRNAs and polymorphic miRNAs
  Randomly Featured Patents
Nitride semiconductor light-emitting device
Image forming apparatus with cleaning means
Low profile electronic device stand pair
Shaver with adjustable long hair trimmer
High performance amphibious aircraft
Shaft alignment collar
Automatic migration of data via a distributed computer network
Compact stand for musical instrument
Method and an apparatus in hot-dip galvanizing
Virtual appliance management