Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Assay for detecting immune responses involving antigen specific cytokine and/or antigen specific cytokine secreting T-cells










Image Number 4 for United States Patent #7897357.

Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytokines (such IFN-.gamma.) and chemokines that upregulate the activity of Th-1 cytokines (such as IFN-.gamma.). In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN-.gamma. rather than direct quantitation of IFN-.gamma. or IFN-.gamma. secreting cells per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8.sup.+ T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8.sup.+ T cells and IFN-.gamma.. Responses as assessed by the MIG assay paralleled those detected by conventional IFN-.gamma. ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN-.gamma. activity or MIG.








 
 
  Recently Added Patents
Methods, systems, and computer readable media for providing priority routing at a diameter node
Multi-point lock system with single position actuation and related methods
Biological array holder
System and method of resolving outliers in NIRS cerebral oximetry
Bidirectional heating cooker
Pneumatic tire with tread having shoulder blocks and crown blocks
Celestial body running vehicle wheel
  Randomly Featured Patents
Magnetic components and their production
Pair of glasses
Electronic display devices and methods
Lateral phase change memory and method therefor
System for removing spurious signatures in motor current signature analysis
Circuit and method for generating a variable frequency clock signal
Temperature detecting apparatus
Sheet feeding apparatus for printing machines
Tension and freely adjustable protective case for remote control unit
Ophthalmic delivery device