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Resolution-enhanced luminescence microscopy










Image Number 14 for United States Patent #7485875.

A resolution-enhanced luminescence microscopy method, wherein a sample is excited so as to luminesce, and thus to emit a given luminescence radiation, by irradiation of exciting radiation and an image of the luminescent sample is obtained, wherein the luminescent sample is transferable from a first state of luminescence, in which first state the sample's excitability for emission of the given luminescence radiation increases up to a maximum value as the exciting radiation power increases, into a second state of luminescence, in which second state the sample has reduced excitability for emission of the given luminescence radiation relative to the first state, wherein the maximum value is assigned to a threshold value of exciting radiation power and the sample is transferable into the second state by irradiation of exciting radiation power above the threshold value, the sample being brought into the first state in partial areas and being brought into the second state in adjacent partial areas by irradiating exciting radiation with an exciting radiation distribution having at least one spatial power maximum above the threshold value, the image of the luminescent sample comprising sample areas being in the first state and sample areas being in the second state, sample areas being in the first state contributing predominantly to the image and the image thus having an enhanced spatial resolution with respect to the exciting radiation distribution.








 
 
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