Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Methods of evaluating viral entry inhibitors using patient derived envelope protein constructs










Image Number 16 for United States Patent #7169551.

The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.








 
 
  Recently Added Patents
Document reuse in a search engine crawler
Estimation of the impulse response of a system on the basis of binary observations
Battery pack
Semiconductor laser
Method and program for verifying operation of processor
Solid-state imaging device, method of manufacturing the same, and electronic equipment
Additive for polyolefin polymerization processes
  Randomly Featured Patents
Physical exerciser
Method of manufacturing V-shaped flash memory
Fuel cell stack with even distributing gas manifolds
Disposable diaper with elastically constricted crotch section
Single transistor cell, method for manufacturing the same, memory circuit composed of single transistor cells, and method for driving the same
Pharmaceutical preparation for treating blood coagulation disorders
Canister
Fruit and vegetable gripper
Gateway for internet telephone
Method and apparatus for directing data packets in a local area network device having a plurality of ports interconnected by a high-speed communication bus