Resources Contact Us Home
Methods of evaluating viral entry inhibitors using patient derived envelope protein constructs

Image Number 16 for United States Patent #7169551.

The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

  Recently Added Patents
Filtering method for improving the data quality of geometric tire measurements
Battery terminal with current sensor
Scalable encoding apparatus, scalable decoding apparatus, scalable encoding method, scalable decoding method, communication terminal apparatus, and base station apparatus
Binary-to-gray converting circuits and gray code counter including the same
Electronic time stamping apparatus for use with an inground transmitter
Preamplifier-to-channel communication in a storage device
Lightning-protective explosion-preventive fastener
  Randomly Featured Patents
Apparatus and method for applying adhesive and release paper to wooden flooring strips
Optical waveguides and methods for making same
Image information detecting apparatus
Fuel injector utilizing flat-seat poppet valves
Grate plate arrangement
Feeding arrangement for mailed items
Liquid crystal display device and driving method thereof
Method for identifying articles and process for maintaining security
Pyrocarbonic acid diesters and the preparation and use thereof