Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Expression vector for use in a one-step purification protocol










Image Number 6 for United States Patent #6482607.

High expression levels and simple downstream processing are essential for the production of recombinant proteins at low cost. We report here a new expression vector which allows production of fusion proteins in Dictyostelium discoideum. We made use of the ability of Discoidin I to bind Sepharose-4B to set up a nearly single step purification procedure. The Discoidin I coding region was fused to several forms of the malaria parasite CSP gene in a Dictyostelium expression vector, allowing intracellular accumulation as well as partial secretion via a pathway unrelated to the endoplasmic reticulum and Golgi. The fusion proteins present in cell extracts were affinity-purified over Sepharose-4B columns. Addition of a signal peptide allowed endoplasmic reticulum targeting and glycosylation of the fusion protein. Inclusion of a thrombin cleavage site allowed to cleave Discoidin from the CSP protein. The use of stable and low cost Sepharose 4B as affinity matrix should allow large-scale preparations.








 
 
  Recently Added Patents
Selecting a converter operating mode of a PA envelope power supply
Basketball
Light emitting device power supply circuit, and light emitting device driver circuit and control method thereof
Television apparatus
Wireless network device including a polarization and spatial diversity antenna system
Field of view matching in a visual prosthesis
Support member, rotation device comprising such a support and rolling bearing assembly including such a detection device
  Randomly Featured Patents
Collapsible bag with square ends formed by triangular portions
Support for photovoltaic arrays
Electro-mechanical actuator and clutch for the same
Housing for a desk telephone stand
Compact simulator system theater
Nudged broadcast orbit drift correction
Liquid ejection apparatus and method
Product and method of forming a gradient density fibrous filter
Self-contained interactive play structure
Process for preparing storage-stable tris(bromophenyl)phosphates