Resources Contact Us Home
Expression vector for use in a one-step purification protocol

Image Number 6 for United States Patent #6482607.

High expression levels and simple downstream processing are essential for the production of recombinant proteins at low cost. We report here a new expression vector which allows production of fusion proteins in Dictyostelium discoideum. We made use of the ability of Discoidin I to bind Sepharose-4B to set up a nearly single step purification procedure. The Discoidin I coding region was fused to several forms of the malaria parasite CSP gene in a Dictyostelium expression vector, allowing intracellular accumulation as well as partial secretion via a pathway unrelated to the endoplasmic reticulum and Golgi. The fusion proteins present in cell extracts were affinity-purified over Sepharose-4B columns. Addition of a signal peptide allowed endoplasmic reticulum targeting and glycosylation of the fusion protein. Inclusion of a thrombin cleavage site allowed to cleave Discoidin from the CSP protein. The use of stable and low cost Sepharose 4B as affinity matrix should allow large-scale preparations.

  Recently Added Patents
Matching geometry generation and display of mammograms and tomosynthesis images
Horizontal aggregations in a relational database management system
Call admission control method and system
Molded surface of a concrete product
Centralized behavioral information system
System and method for customized prompting
  Randomly Featured Patents
Methods and systems for phase current reconstruction of AC drive systems
Scale control with terpolymers containing vinyl alcohol
Engine block structure
Semiconductor memory device with reduced current consumption
Data display arrangement
Protective layers of germanium ceramics
Self-supporting level measurement device
Process for separating 1-dodecene and 1-tetradecene from an aluminum alkyl chain growth product
Railway coupler
Device for controlling feeder blowing air and feeder suction air in a sheet feeder of a printing machine