Resources Contact Us Home
Expression vector for use in a one-step purification protocol

Image Number 6 for United States Patent #6482607.

High expression levels and simple downstream processing are essential for the production of recombinant proteins at low cost. We report here a new expression vector which allows production of fusion proteins in Dictyostelium discoideum. We made use of the ability of Discoidin I to bind Sepharose-4B to set up a nearly single step purification procedure. The Discoidin I coding region was fused to several forms of the malaria parasite CSP gene in a Dictyostelium expression vector, allowing intracellular accumulation as well as partial secretion via a pathway unrelated to the endoplasmic reticulum and Golgi. The fusion proteins present in cell extracts were affinity-purified over Sepharose-4B columns. Addition of a signal peptide allowed endoplasmic reticulum targeting and glycosylation of the fusion protein. Inclusion of a thrombin cleavage site allowed to cleave Discoidin from the CSP protein. The use of stable and low cost Sepharose 4B as affinity matrix should allow large-scale preparations.

  Recently Added Patents
System and method for hedging risks in commercial leases
Backlight assembly comprising a light-sourcing unit fixing frame with a lateral extension portion and a fixing protrusion which interlocks the light-sourcing unit fixing frame to a housing
Delegation based mobility management
Reducing head and tail duplication in stored data
Bioactive graded zirconia-based structures
Inspecting device, inspecting method, and method for manufacturing optical fiber
Audio content enhancement using bandwidth extension techniques
  Randomly Featured Patents
Gas spring locking mechanism
Method and apparatus for manufacturing a spherical hollow body
Battery for a mowing machine
Semiconductor integrated circuit designing method, semiconductor integrated circuit device, and electronic device
System for processing information, device for processing information, and program
Phosphinimine modification of organic polymers and silicones
Integrated circuit isolation process
Pharmaceutical composition comprising temozolomide ester
Male genitalia support garment
Method and apparatus for switchably selecting an integrated circuit operating mode