Resources Contact Us Home
Expression vector for use in a one-step purification protocol

Image Number 6 for United States Patent #6482607.

High expression levels and simple downstream processing are essential for the production of recombinant proteins at low cost. We report here a new expression vector which allows production of fusion proteins in Dictyostelium discoideum. We made use of the ability of Discoidin I to bind Sepharose-4B to set up a nearly single step purification procedure. The Discoidin I coding region was fused to several forms of the malaria parasite CSP gene in a Dictyostelium expression vector, allowing intracellular accumulation as well as partial secretion via a pathway unrelated to the endoplasmic reticulum and Golgi. The fusion proteins present in cell extracts were affinity-purified over Sepharose-4B columns. Addition of a signal peptide allowed endoplasmic reticulum targeting and glycosylation of the fusion protein. Inclusion of a thrombin cleavage site allowed to cleave Discoidin from the CSP protein. The use of stable and low cost Sepharose 4B as affinity matrix should allow large-scale preparations.

  Recently Added Patents
Coordinate locating method and apparatus
Composite filtration membranes and methods of preparation thereof
Universal tablet and smartphone holder
Non-aqueous solution process for the preparation of cross-linked polymers
Synthesis of 8-amino boron dipyrromethenes having blue fluorescence
Device and method for unified codes
Onium salt-containing polymer
  Randomly Featured Patents
Electronic device package
Apparatus for and a method of clock tree synthesis allocation wiring
Thermal transfer recording device and method therefor
Foldable and stackable device for winding flexible tubular pipes
Battery feed circuit
Process for installing a pipe liner
Optical fiber cable
Distributing device for the manufacture of multi-layer sheets
X-ray imaging method and apparatus to reduce X-ray exposure of subject regions other than the diagnostically relevant subject region
Hydrotreating process employing a pretreated alumina containing material