Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Expression vector for use in a one-step purification protocol










Image Number 6 for United States Patent #6482607.

High expression levels and simple downstream processing are essential for the production of recombinant proteins at low cost. We report here a new expression vector which allows production of fusion proteins in Dictyostelium discoideum. We made use of the ability of Discoidin I to bind Sepharose-4B to set up a nearly single step purification procedure. The Discoidin I coding region was fused to several forms of the malaria parasite CSP gene in a Dictyostelium expression vector, allowing intracellular accumulation as well as partial secretion via a pathway unrelated to the endoplasmic reticulum and Golgi. The fusion proteins present in cell extracts were affinity-purified over Sepharose-4B columns. Addition of a signal peptide allowed endoplasmic reticulum targeting and glycosylation of the fusion protein. Inclusion of a thrombin cleavage site allowed to cleave Discoidin from the CSP protein. The use of stable and low cost Sepharose 4B as affinity matrix should allow large-scale preparations.








 
 
  Recently Added Patents
System and method for removing oxide from a sensor clip assembly
Semiconductor memory system having ECC circuit and controlling method thereof
Reliable event broadcaster with multiplexing and bandwidth control functions
Identification of websites that break out of frames
Software self-checking systems and methods
Method for processing an algae medium containing algae microorganisms to produce algal oil and by-products
Estimating travel time
  Randomly Featured Patents
Power generating block provided with thermoelectric generation unit
Automatic self-guiding lawn mower and mowing method
Foot pillow
Method of drilling a directonal well bore
Semiconductor nonvolatile memory device and method of producing the same
MAC address notification method in MPOA systems and MPOA server for the same
Regeneration flow distribution device
Genetic Markers for breast, ovarian, and prostatic cancer
Silicon-based visible and near-infrared optoelectric devices
Hearing aid having improved RF immunity to RF electromagnetic interference produced from a wireless communications device