Resources Contact Us Home
Expression vector for use in a one-step purification protocol

Image Number 6 for United States Patent #6482607.

High expression levels and simple downstream processing are essential for the production of recombinant proteins at low cost. We report here a new expression vector which allows production of fusion proteins in Dictyostelium discoideum. We made use of the ability of Discoidin I to bind Sepharose-4B to set up a nearly single step purification procedure. The Discoidin I coding region was fused to several forms of the malaria parasite CSP gene in a Dictyostelium expression vector, allowing intracellular accumulation as well as partial secretion via a pathway unrelated to the endoplasmic reticulum and Golgi. The fusion proteins present in cell extracts were affinity-purified over Sepharose-4B columns. Addition of a signal peptide allowed endoplasmic reticulum targeting and glycosylation of the fusion protein. Inclusion of a thrombin cleavage site allowed to cleave Discoidin from the CSP protein. The use of stable and low cost Sepharose 4B as affinity matrix should allow large-scale preparations.

  Recently Added Patents
Method and apparatus for detecting and tracking vehicles
Potato cultivar F10
Surface acoustic wave resonator for down-hole applications
Storage apparatus and method including page discard processing for primary and secondary volumes configured as a copy pair
Undercabinet plug-in mount
Remote management of networked systems using secure modular platform
Method for producing purified influenza virus antigen
  Randomly Featured Patents
Real-time monitor mechanism for heterogeneous production lines
Fiber optic module
Drop-in anchor
Fiber optic connector
Computer evaluation of crop related industries
Video-supported planning of equipment installation and/or room design
Light modulation by frustration of total internal reflection
Method and apparatus for recovering from system bus transaction errors
Plug valve with hydraulically actuated seals
Pair of handles for pliers