Resources Contact Us Home
Detection of molecular interactions by reporter subunit complementation

Image Number 13 for United States Patent #6342345.

Methods and compositions for detecting molecular interactions, particularly protein-protein interactions, are provided. The invention allows detection of such interactions in living cells or in vitro. Detection of molecular interactions in living cells is not limited to the nuclear compartment, but can be accomplished in the cytoplasm, cell surface, organelles, or between these entities. In one embodiment, the method utilizes novel compositions comprising fusion proteins between the molecules of interest and two or more inactive, weakly-complementing .beta.-galactosidase mutants. Association between the molecules of interest brings the complementing .beta.-galactosidase mutants into proximity so that complementation occurs and active .beta.-galactosidase is produced. The active .beta.-galactosidase may be detected by methods well-known in the art. Among the uses of the invention are the study of protein-protein interactions, functional genomics, agonist and antagonist screening and drug discovery.

  Recently Added Patents
Magnesium metal-air battery
Systems and methods for arranging and labeling cardiac episodes
Method and apparatus for providing auto-completion of information
Substrate processing apparatus and display method of substrate processing apparatus
Automatic misalignment balancing scheme for multi-patterning technology
Method and apparatus of communication using soft decision
Circuit for and method of enabling communication of cryptographic data
  Randomly Featured Patents
Electronic edge and center locator and method of using same
PRO4320 polypeptide
Complex amide carboxylate lubricant rust inhibitor additive for metal working fluids
Poker chip with alternating double stripe and card suit symbol patterns
Process for producing a cover with a glass pane and electrical function elements
Main shaft drive for machine tool
Loudspeaker having acoustic impedance frontal loading element
Elevator safety arrangement having safety spaces
Seat belt utilization recorder
Alumina chlorination