Resources Contact Us Home
Detection of molecular interactions by reporter subunit complementation

Image Number 13 for United States Patent #6342345.

Methods and compositions for detecting molecular interactions, particularly protein-protein interactions, are provided. The invention allows detection of such interactions in living cells or in vitro. Detection of molecular interactions in living cells is not limited to the nuclear compartment, but can be accomplished in the cytoplasm, cell surface, organelles, or between these entities. In one embodiment, the method utilizes novel compositions comprising fusion proteins between the molecules of interest and two or more inactive, weakly-complementing .beta.-galactosidase mutants. Association between the molecules of interest brings the complementing .beta.-galactosidase mutants into proximity so that complementation occurs and active .beta.-galactosidase is produced. The active .beta.-galactosidase may be detected by methods well-known in the art. Among the uses of the invention are the study of protein-protein interactions, functional genomics, agonist and antagonist screening and drug discovery.

  Recently Added Patents
Recovering a database to any point-in-time in the past with guaranteed data consistency
Method of fabricating crystal unit, crystal unit fabrication mask, and crystal unit package
Exposure apparatus, exposure method, and device manufacturing method
Modular authoring and visualization of rules using trees
Method and system for fail-safe call survival
Antenna array system
Methods and systems for managing electronic messages
  Randomly Featured Patents
Fluid pressure generating means
Garage and vehicle protector
System for generating permalink of mash-up map
Alkylamino mercaptoalkylamides and their use as a reducing agent in a process for the permanent deformation of hair
Ceiling fan support
Guiding rail for a cabinet pull-out part
Insulated pad conditioner and method of using same
Sea wave energy conversion
Manufacturing method of microelectromechanical system
Automatic lock for farm equipment transport wheel