Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Recombinant materials and methods for production of limonene hydroxylases










Image Number 3 for United States Patent #6194185.

cDNA encoding (-)-limonene-6-hydroxylase from spearmint and (-)-limonene-3-hydroxylase from peppermint have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences are provided which code for the expression of (-)-limonene-6-hydroxylase from spearmint (SEQ ID No:1, from Mentha spicata) and (-)-limonene-3-hydroxylase from peppermint (SEQ ID No:3 and SEQ ID No:5, from Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for limonene hydroxylase or for a base sequence sufficiently complementary to at least a portion of the limonene hydroxylase DNA or RNA to enable hybridization therewith (e.g., antisense limonene hydroxylase RNA or fragments of complementary limonene hydroxylase DNA which are useful as polymerase chain reaction primers or as probes for limonene hydroxylase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding limonene hydroxylase. Thus, systems and methods are provided for the recombinant expression of limonene hydroxylase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant limonene hydroxylase (or of the primary enzyme products, trans-carveol or trans-isopiperitenol, respectively) for subsequent use, to obtain expression or enhanced expression of limonene hydroxylase in plants to attain enhanced production of trans-carveol or trans-isopiperitenol as a predator or pathogen defense mechanism, or may be otherwise employed for the regulation or expression of limonene hydroxylase or the production of trans-carveol or trans-isopiperitenol.








 
 
  Recently Added Patents
Semiconductor process
Projection screen and projection system having the same
Polymer composites having highly dispersed carbon nanotubes
Shot scent dispenser
Emergency whistle, flashlight, and compass
Field device for determining or monitoring a physical or chemical process variable
Portable electronic device holder and tether
  Randomly Featured Patents
Bulk material storage car with sensor
Measuring device for amount of charge of developer
Methods and systems for mass spectrometry
UV-stabilized polycarbonate moldings
Method of forming a pattern on a substrate having a step change in height
Semiconductor integrated circuit
Control system for a voltage-type inverter
Chromatography media sensor system
Carrier film and method for its production
Bed pan apparatus