Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Recombinant materials and methods for production of limonene hydroxylases










Image Number 3 for United States Patent #6194185.

cDNA encoding (-)-limonene-6-hydroxylase from spearmint and (-)-limonene-3-hydroxylase from peppermint have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences are provided which code for the expression of (-)-limonene-6-hydroxylase from spearmint (SEQ ID No:1, from Mentha spicata) and (-)-limonene-3-hydroxylase from peppermint (SEQ ID No:3 and SEQ ID No:5, from Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for limonene hydroxylase or for a base sequence sufficiently complementary to at least a portion of the limonene hydroxylase DNA or RNA to enable hybridization therewith (e.g., antisense limonene hydroxylase RNA or fragments of complementary limonene hydroxylase DNA which are useful as polymerase chain reaction primers or as probes for limonene hydroxylase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding limonene hydroxylase. Thus, systems and methods are provided for the recombinant expression of limonene hydroxylase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant limonene hydroxylase (or of the primary enzyme products, trans-carveol or trans-isopiperitenol, respectively) for subsequent use, to obtain expression or enhanced expression of limonene hydroxylase in plants to attain enhanced production of trans-carveol or trans-isopiperitenol as a predator or pathogen defense mechanism, or may be otherwise employed for the regulation or expression of limonene hydroxylase or the production of trans-carveol or trans-isopiperitenol.








 
 
  Recently Added Patents
Beaconing and superframe structure for millimeter wave wireless technologies
Mixture, especially spinning solution
Negative active material for a rechargeable lithium battery, a method of preparing the same, and a rechargeable lithium battery comprising the same
Display screen with graphical user interface
Electro-optical device, method of manufacturing the same, and electronic apparatus
Pressure-sensitive adhesive composition for optical films, pressure-sensitive adhesive optical film and image display
Apparatus for focus beam analysis of high power lasers
  Randomly Featured Patents
Multi-directional input unit
System for unloading a memory cartridge
Vehicle roof rack loading mechanism
Compact disc loading and unloading device with rotationally actuated movable disc support
Oxide-like seasoning for dielectric low k films
Mechanically-coupled microphone-speaker unit and intercom system
Changing codec information to provide voice over internet protocol (VoIP) terminal with coloring service
Non-volatile memory and method with multi-stream updating
Apparatus for cutting cushion-like packaging material
Folding knife with safety for blade