Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Recombinant materials and methods for production of limonene hydroxylases










Image Number 3 for United States Patent #6194185.

cDNA encoding (-)-limonene-6-hydroxylase from spearmint and (-)-limonene-3-hydroxylase from peppermint have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences are provided which code for the expression of (-)-limonene-6-hydroxylase from spearmint (SEQ ID No:1, from Mentha spicata) and (-)-limonene-3-hydroxylase from peppermint (SEQ ID No:3 and SEQ ID No:5, from Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for limonene hydroxylase or for a base sequence sufficiently complementary to at least a portion of the limonene hydroxylase DNA or RNA to enable hybridization therewith (e.g., antisense limonene hydroxylase RNA or fragments of complementary limonene hydroxylase DNA which are useful as polymerase chain reaction primers or as probes for limonene hydroxylase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding limonene hydroxylase. Thus, systems and methods are provided for the recombinant expression of limonene hydroxylase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant limonene hydroxylase (or of the primary enzyme products, trans-carveol or trans-isopiperitenol, respectively) for subsequent use, to obtain expression or enhanced expression of limonene hydroxylase in plants to attain enhanced production of trans-carveol or trans-isopiperitenol as a predator or pathogen defense mechanism, or may be otherwise employed for the regulation or expression of limonene hydroxylase or the production of trans-carveol or trans-isopiperitenol.








 
 
  Recently Added Patents
Compositions and methods for lowering triglycerides in a subject on concomitant statin therapy
Computerized apparatus for identifying industries for potential transfer of a job function
ActRIIB proteins and variants and uses therefore relating to utrophin induction for muscular dystrophy therapy
Data transfer device and data transfer method
System and method for improving text input in a shorthand-on-keyboard interface
Method for testing multi-chip stacked packages
Method for adjusting link speed and computer system using the same
  Randomly Featured Patents
Ballnose end mill
Method for crystal growth of beta barium boratean
Injection mold having a movable slide member
Method of fabricating a field effect semiconductor device having a self-aligned structure
Fiber tip applicator
Spacer and spacer frame for an insulating glazing unit and method of making same
Bonded semiconductor integrated circuit device
Heating windshield wiper shroud system
Flooring sheet
Rolling bellows for pneumatic cushioning of a vehicle