Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Recombinant materials and methods for production of limonene hydroxylases










Image Number 3 for United States Patent #6194185.

cDNA encoding (-)-limonene-6-hydroxylase from spearmint and (-)-limonene-3-hydroxylase from peppermint have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences are provided which code for the expression of (-)-limonene-6-hydroxylase from spearmint (SEQ ID No:1, from Mentha spicata) and (-)-limonene-3-hydroxylase from peppermint (SEQ ID No:3 and SEQ ID No:5, from Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for limonene hydroxylase or for a base sequence sufficiently complementary to at least a portion of the limonene hydroxylase DNA or RNA to enable hybridization therewith (e.g., antisense limonene hydroxylase RNA or fragments of complementary limonene hydroxylase DNA which are useful as polymerase chain reaction primers or as probes for limonene hydroxylase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding limonene hydroxylase. Thus, systems and methods are provided for the recombinant expression of limonene hydroxylase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant limonene hydroxylase (or of the primary enzyme products, trans-carveol or trans-isopiperitenol, respectively) for subsequent use, to obtain expression or enhanced expression of limonene hydroxylase in plants to attain enhanced production of trans-carveol or trans-isopiperitenol as a predator or pathogen defense mechanism, or may be otherwise employed for the regulation or expression of limonene hydroxylase or the production of trans-carveol or trans-isopiperitenol.








 
 
  Recently Added Patents
Dual-leadframe multi-chip package and method of manufacture
Printing apparatus having extendable functionality and method thereof
Method of forming a power supply controller and structure therefor
Method and apparatus for executing load distributed printing
Method and apparatus for variable accuracy inter-picture timing specification for digital video encoding
1,3-diiodohydantoin compound and production method thereof
PMI feedback with codebook interpolation
  Randomly Featured Patents
Solid state imaging apparatus
Anti-weaving conveyor belt
Method of fabricating capacitors of a memory cell array
Worm gear drive
Method for treatment of restenosis
Pivotable tray for a pallet truck
Control system for battery powered heating device
Apparatus for signal power loss reduction in RF communication systems
Package components for radiochemical sterilization
High efficiency insulated water heater and method for making same