Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Recombinant materials and methods for production of limonene hydroxylases










Image Number 3 for United States Patent #6194185.

cDNA encoding (-)-limonene-6-hydroxylase from spearmint and (-)-limonene-3-hydroxylase from peppermint have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences are provided which code for the expression of (-)-limonene-6-hydroxylase from spearmint (SEQ ID No:1, from Mentha spicata) and (-)-limonene-3-hydroxylase from peppermint (SEQ ID No:3 and SEQ ID No:5, from Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for limonene hydroxylase or for a base sequence sufficiently complementary to at least a portion of the limonene hydroxylase DNA or RNA to enable hybridization therewith (e.g., antisense limonene hydroxylase RNA or fragments of complementary limonene hydroxylase DNA which are useful as polymerase chain reaction primers or as probes for limonene hydroxylase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding limonene hydroxylase. Thus, systems and methods are provided for the recombinant expression of limonene hydroxylase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant limonene hydroxylase (or of the primary enzyme products, trans-carveol or trans-isopiperitenol, respectively) for subsequent use, to obtain expression or enhanced expression of limonene hydroxylase in plants to attain enhanced production of trans-carveol or trans-isopiperitenol as a predator or pathogen defense mechanism, or may be otherwise employed for the regulation or expression of limonene hydroxylase or the production of trans-carveol or trans-isopiperitenol.








 
 
  Recently Added Patents
Signal conversion control circuit for touch screen and method thereof
Methods, devices and computer program products providing for establishing a model for emulating a physical quantity which depends on at least one input parameter, and use thereof
Amide derivatives, process for preparation thereof and use thereof as insecticide
Liquid crystal display device and driving method thereof
Drug delivery system
Tiered cost model for access to a computer network
Electrical event detection device and method of detecting and classifying electrical power usage
  Randomly Featured Patents
Radio paging receiver capable of readily confirming a state of a non read message
Flexible-disc coupling
Image processing apparatus for editing a dynamic image having a first and a second hierarchy classifying and synthesizing plural sets of: frame images displayed in a tree structure
Voltage division method for protection against load dump conditions
Method and apparatus for simultaneous and continuous delivery of liquids from a plurality of individual containers to a dispensing device
Facsimile receiving apparatus
Funicular system including haul rope grip assembly
Method for the temperature treatment of extrusion tools
Peptides for the diagnosis of HTLV-III antibodies, their preparation and use
Method and apparatus for ink jet printing