Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence










Image Number 8 for United States Patent #5624824.

It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide ("external guide sequence", or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.








 
 
  Recently Added Patents
Comparison of visual information
Fast port switching in an audiovisual receiver by use of port pre-authentication by a partial PHY decoder core
Modular utility rack
Nonvolatile semiconductor memory device
Semiconductor IC including pulse generation logic circuit
Antibodies to Clostridium difficile toxins
Flash multi-level threshold distribution scheme
  Randomly Featured Patents
Connecting device for an equipment unit suspended from a system which subjects said unit to differential movements of displacement
Driving method and driving apparatus of a solenoid and solenoid driving control apparatus
Palletizer
Aircraft ram jet engine for supersonic and/or hypersonic flight
Method for allocating high voltage electrical energy to vehicle systems during propulsion
Small-sized printer
Machine for the processing of foodstuffs or for the preparation of pharmaceutical and chemical products
Method of fabricating active elements for a laser source
Absorbent article
Apparatus and method for transmitting and receiving packets in a mobile communication system supporting hybrid automatic repeat request