Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence










Image Number 5 for United States Patent #5624824.

It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide ("external guide sequence", or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.








 
 
  Recently Added Patents
Granulated sweetening composition
Phone messaging using audio streams
Color image forming apparatus with contact control of process units
Lifting apparatus
Image processing apparatus and method configured to calculate defocus amount of designated area
Caching techniques
Method and apparatus for token-based context caching
  Randomly Featured Patents
Switch assembly having indicator
Method and plant for separating soap from black liquor using a pressure screen
Method for manufacturing pattern formed body
Telephone apparatus housing
Golf club head with three-dimensional alignment aid and method of manufacture
Apparatus for roasting and grilling of foodstuffs
Solid electrolyte additive
Feed comb arrangement
Multistep process for the liquid phase ammoximation of carbonyl compounds
Auto promotion and demotion of conference calls