Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence










Image Number 4 for United States Patent #5624824.

It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide ("external guide sequence", or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.








 
 
  Recently Added Patents
Light emitting diode package and method of fabricating the same
Node and wireless sensor network comprising the node
Stacked magnetoresistance device responsive to a magnetic field generally perpendicular to a side face running along a channel of the device
Malicious attack detection and analysis
Voltage regulators with improved wake-up response
Process and apparatus for producing composite material that includes carbon nanotubes
Silicas and alkali metal salt compositions, detergents formed from such compositions and method of forming such compositions and detergents
  Randomly Featured Patents
Optical gain equalizer, and optical amplifier and wavelength-division multiplex transmitter both comprising the optical gain equalizer
Interior extension with floating corners
Broadband corrugated horn radiator
Eyeglasses
Cemented carbide employing a refractory metal binder and process for producing same
Pre-wrapped two-piece ring airfoil projectile of non-hazardous material
Pen
Mechanism for handling persistent requests from stateless clients
Scorch extending curing/crosslinking compositions
Method of enhancing the cooking of wood chips for pulp production