Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Molecular cloning and expression of biologically-active diphtheria toxin receptor










Image Number 7 for United States Patent #5366874.

DT-resistant wild-type mouse L-M cells were co-transfected with a cDNA library constructed from RNA of highly toxin-sensitive monkey Vero cells and with a neomycin resistance gene. One DT-sensitive (DT.sup.S) colony was isolated from 8,000 stably-transfected neomycin-resistant L-M colonies screened by replica plate assays. The purified DT.sup.S mouse cells are highly toxin-sensitive (.about.1,000-fold more so than L-M cells and only .about.10-fold less than Vero cells) and are protected from DT toxicity on incubation with a nontoxic competitive DT inhibitor (CRM 197). Importantly, the cell surface receptors on DT.sup.S cells specifically bind radioiodinated DT, which is inhibited by unlabelled DT and the DT receptor-binding domain (HA6DT). A plasmid conferring DT-sensitivity (pDTS) was rescued from DT.sup.S cells, and the screening procedure repeated until a single cDNA encoding the DT receptor was isolated. The cDNA is predicted to encode an integral membrane protein that corresponds to the precursor of a human heparin-binding EGF-like growth factor. The DT sensitivity protein is thus a growth factor precursor which is exploited by DT, thus allowing the toxin to enter the cell.








 
 
  Recently Added Patents
Discharge circuit and method
Method for switching between virtualized and non-virtualized system operation
Housekeeping cart
Compositions and methods for producing isoprene
Plants and seeds of corn variety CV092363
Puncture resistant fabric
Signal phase-based location of network nodes
  Randomly Featured Patents
Control apparatus, dual chip inverter and single chip inverter of AC motors
Signal transmitter comprising a hall sensor integrated in a master cylinder
Combining transform coefficient decoding with variable length decoding
One-way measuring element for analyzing gaseous or liquid samples
Plate laminate type heat exchanger
Polyurethane foams having reduced flammability
Two wire autobias vehicular microphone system having user input functionality and method of forming same
Case packing machine and method
Plastic closure with barrier coating
Photocopying machines