Resources Contact Us Home
Molecular cloning and expression of biologically-active diphtheria toxin receptor

Image Number 7 for United States Patent #5366874.

DT-resistant wild-type mouse L-M cells were co-transfected with a cDNA library constructed from RNA of highly toxin-sensitive monkey Vero cells and with a neomycin resistance gene. One DT-sensitive (DT.sup.S) colony was isolated from 8,000 stably-transfected neomycin-resistant L-M colonies screened by replica plate assays. The purified DT.sup.S mouse cells are highly toxin-sensitive (.about.1,000-fold more so than L-M cells and only .about.10-fold less than Vero cells) and are protected from DT toxicity on incubation with a nontoxic competitive DT inhibitor (CRM 197). Importantly, the cell surface receptors on DT.sup.S cells specifically bind radioiodinated DT, which is inhibited by unlabelled DT and the DT receptor-binding domain (HA6DT). A plasmid conferring DT-sensitivity (pDTS) was rescued from DT.sup.S cells, and the screening procedure repeated until a single cDNA encoding the DT receptor was isolated. The cDNA is predicted to encode an integral membrane protein that corresponds to the precursor of a human heparin-binding EGF-like growth factor. The DT sensitivity protein is thus a growth factor precursor which is exploited by DT, thus allowing the toxin to enter the cell.

  Recently Added Patents
Solid-state imaging apparatus and imaging system
Electronic component and reflected-wave cancelling method
Segmentation of a product markup image based on color and color differences
Method and system for presenting live video from video capture devices on a computer monitor
Negative active material for a rechargeable lithium battery, a method of preparing the same, and a rechargeable lithium battery comprising the same
Circuit board having semiconductor chip embedded therein
Image processing system, image processing method and program for image processing
  Randomly Featured Patents
Method of and apparatus for reading
Process for imparting lubricity to fiberfill fiber
Multivariable artifact assessment
Venturi device
Programmable byte wise MPEG systems layer parser
Display device
Apparatus for extracting a variable from two quadrature signals being functions of the variable
Data processing system, data processing apparatus and control method for a data processing apparatus
Image forming apparatus with laser calibration during ramp-up period of an optical device
Procedure for operating an elevator, and an elevator machinery