Resources Contact Us Home
Molecular cloning and expression of biologically-active diphtheria toxin receptor

Image Number 7 for United States Patent #5366874.

DT-resistant wild-type mouse L-M cells were co-transfected with a cDNA library constructed from RNA of highly toxin-sensitive monkey Vero cells and with a neomycin resistance gene. One DT-sensitive (DT.sup.S) colony was isolated from 8,000 stably-transfected neomycin-resistant L-M colonies screened by replica plate assays. The purified DT.sup.S mouse cells are highly toxin-sensitive (.about.1,000-fold more so than L-M cells and only .about.10-fold less than Vero cells) and are protected from DT toxicity on incubation with a nontoxic competitive DT inhibitor (CRM 197). Importantly, the cell surface receptors on DT.sup.S cells specifically bind radioiodinated DT, which is inhibited by unlabelled DT and the DT receptor-binding domain (HA6DT). A plasmid conferring DT-sensitivity (pDTS) was rescued from DT.sup.S cells, and the screening procedure repeated until a single cDNA encoding the DT receptor was isolated. The cDNA is predicted to encode an integral membrane protein that corresponds to the precursor of a human heparin-binding EGF-like growth factor. The DT sensitivity protein is thus a growth factor precursor which is exploited by DT, thus allowing the toxin to enter the cell.

  Recently Added Patents
Semiconductor device and method of manufacturing the same
Accordion bioreactor
Medical capsule housing formed by thermal welding
Non-volatile semiconductor memory and data processing method in non-volatile semiconductor memory
Photographic printing paper and method of making same
Base station device and wireless communication method
Systems and method for automatic color plane misregistration calibration
  Randomly Featured Patents
Data shifting circuit capable of an original data width rotation and a double data width rotation
Aeration drum for composting toilet
Board game computing device
Bond strength of coatings to ceramic components
Ultrasonic testing of seams
Multi-layer sheet having a weatherable surface layer
Scintillation liquid compatible with high performance liquid chromatography solvents
Downhole hydraulic control system with failsafe features
Light weight golf club shaft
Dental root-canal broach handle