Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Molecular cloning and expression of biologically-active diphtheria toxin receptor










Image Number 7 for United States Patent #5366874.

DT-resistant wild-type mouse L-M cells were co-transfected with a cDNA library constructed from RNA of highly toxin-sensitive monkey Vero cells and with a neomycin resistance gene. One DT-sensitive (DT.sup.S) colony was isolated from 8,000 stably-transfected neomycin-resistant L-M colonies screened by replica plate assays. The purified DT.sup.S mouse cells are highly toxin-sensitive (.about.1,000-fold more so than L-M cells and only .about.10-fold less than Vero cells) and are protected from DT toxicity on incubation with a nontoxic competitive DT inhibitor (CRM 197). Importantly, the cell surface receptors on DT.sup.S cells specifically bind radioiodinated DT, which is inhibited by unlabelled DT and the DT receptor-binding domain (HA6DT). A plasmid conferring DT-sensitivity (pDTS) was rescued from DT.sup.S cells, and the screening procedure repeated until a single cDNA encoding the DT receptor was isolated. The cDNA is predicted to encode an integral membrane protein that corresponds to the precursor of a human heparin-binding EGF-like growth factor. The DT sensitivity protein is thus a growth factor precursor which is exploited by DT, thus allowing the toxin to enter the cell.








 
 
  Recently Added Patents
System and method for optimizing use of plug-in air conditioners and portable heaters
Digital photographing apparatus and control method for evaluating validity of an auto-focus operation
Shower bench
Atmospheric treater with roller confined discharge chamber
Lead frame array package with flip chip die attach
Reticle for a riflescope or other projectile-weapon aiming device
Therapeutic compositions and methods
  Randomly Featured Patents
Centrifugal flow fan and fan/orifice assembly
Method for stimulating dendritic cells and cell product thus obtained for the autologous immunotherapy of solid human tumours
High gloss polyethylene terephthalate reinforced resin compositions
Injection apparatus and method with automatic aspiration feature
Connectorless sea train
Storage/retrieval system for document
Infrared spectrometer
Producing a final modified digital image using a source digital image and a difference digital image
Capacitive proximity switch, and domestic appliance equipped with the same
Process for purifying waste water