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Biomolecules covalently immobilized with a high bound specific biological activity and method of preparing same

Image Number 3 for United States Patent #5200471.

A biologically active adduct support, prepared from an azlactone-functional polymeric support and biomolecules covalently immobilized thereon, is disclosed. The biomolecules are covalently immobilized in the presence of polyanionic salt using a method which achieves both high density and optimized bound specific biological activity. Preferably, the immobilization occurs in the presence of an azlactone quencher.

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