Resources Contact Us Home
Highly sensitive enzymatic assay method

Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.

  Recently Added Patents
Discovery, analysis, and visualization of dependencies
Differential ring oscillator and method for calibrating the differential ring oscillator
Client-managed group communication sessions within a wireless communications system
Method and apparatus for providing seamless call handoff between networks that use dissimilar transmission methods
Recovery of a hot-pluggable serial communication link
Transfer of digital data through an isolation
Field device configuration system
  Randomly Featured Patents
Methods for treatment and disposal of regulated medical waste
Edible cone
Data processing method for sampling data from sets of original data
Image forming method, a photoreceptor used for the apparatus, and an image forming unit
Reliable field-effect transistor timer circuit
Strip drying or curing oven
Computer system for enhanced taxonomy management
Apparatus for winding helical wire coils
Tilt-box control system
Agile network protocol for secure communications with assured system availability