Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Highly sensitive enzymatic assay method










Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.








 
 
  Recently Added Patents
Wideband antenna
Controller
Device for data routing in networks
Silicas and alkali metal salt compositions, detergents formed from such compositions and method of forming such compositions and detergents
Sending notification of event
Circuit arrangement for a piezo transformer, and method therefor
Rotation angle sensor
  Randomly Featured Patents
Optical digital to analog converter
Bandpass filter for differential signal, and multifrequency antenna provided with same
Asynchronous, independent and multiple process shared memory system in an adaptive computing architecture
Multimeric forms of members of the steroid/thyroid superfamily of receptors with the ultraspiracle receptor
Air nozzle
Resilient U-clip assembly
Ink particle jetting device for multi-nozzle ink jet printer
Method of preventing combustion by applying an aqueous superabsorbent polymer composition
Digital map position transfer method
Heat exchanger for vehicle