Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Highly sensitive enzymatic assay method










Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.








 
 
  Recently Added Patents
Handling errors in a data processing system
Systems and methods for controlling phasing of advancing substrates in absorbent article converting lines
Backside structure and methods for BSI image sensors
Power-saving receiver
Pixel interleaving configurations for use in high definition electronic sign displays
Combination of the application of antibodies for immunostimulation together with glucocorticoids
Front exterior of an automotive tail lamp
  Randomly Featured Patents
Thin film transistors of a thin film transistor liquid crystal display and method for fabricating the same
Sheet moulding compound and a top for it
Scheme to test/repair multiple large RAM blocks
Synthesis of UDP-glucose: N-acylsphingosine glucosyltransferase inhibitors
Method and apparatus for accelerating test case development
Carboxylate coated chloride salt ice melters
Intake control system for internal combustion engine
Plug connector
Computing device system and information managing method for rearrangement of data based on access characteristic related to a task
Flow control assembly having a fixed flow control device and an adjustable flow control device