Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Highly sensitive enzymatic assay method










Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.








 
 
  Recently Added Patents
Vacuum cleaner filter adapter ring
Image coding apparatus and image decoding apparatus
Feature management of a communication device
Methods and apparatus for address translation functions
People engine optimization
Display screen or portion thereof with animated graphical user interface
Dispenser
  Randomly Featured Patents
Lithography system comprising a converter plate and means for protecting the converter plate
Antibodies to high mobility group-1(HMGB1) B-box polypeptides
Process for the dissolution of peptides in non-aqueous and mixed non-aqueous/aqueous systems
Method of reducing the incidence of medical complications using implantable microstimulators
Macrocyclic filled compositions convertible to polyester composites
Vitreous silica product manufactured via a sol-gel process using a polymer additive
Multi-layer sheet feeder
Dispenser apparatus
Stopper device
Integrated circuit device