Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Highly sensitive enzymatic assay method










Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.








 
 
  Recently Added Patents
Cryptography on a simplified elliptical curve
Gestures for presentation of different views of a system diagram
Developing device
Interface circuit and interface system
Selenium/group 3A ink and methods of making and using same
Account and customer creation in an on-line banking model
High performance actuator motor
  Randomly Featured Patents
Treadmill resistance apparatus
Sunroof latch
Vehicle theft preventing seat cushion frame
Method and apparatus for automatically biasing a push-pull amplifier
Yard sign
Method of treating dry eye disease with purinergic receptor agonists
Divergence feedback in a linear fiber optic analog transmission system and method
Apparatus for measuring head and spine movement
Dual motor aligner
Control system for variable-pitch boat propeller