Resources Contact Us Home
Highly sensitive enzymatic assay method

Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.

  Recently Added Patents
Method for determining a signal component for reducing noise in an input signal
Media tag recommendation technologies
Key allocation when tracing data processing systems
System and method for extraction of factoids from textual repositories
Stacked single crystal compound semiconductor substrates
Process for producing propylene oxide using a pretreated epoxidation catalyst
  Randomly Featured Patents
Grooming brush for animals
Belt cleaning system and method for laser cutting device
Identification of objects in a 3D video using non/over reflective clothing
Polyester containers having a reduced coefficient of friction
Multi-purpose FES system
Control system and method for processing jewelry and the like
Preventing deadlocks
Pivot shaft fixing structure with projection integral with cover member and data storage device having the same
Hand-held and operated seeder apparatus
Wavefront aberration compensation element, optical pickup, and optical disk apparatus