Resources Contact Us Home
Highly sensitive enzymatic assay method

Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.

  Recently Added Patents
Family of pain producing substances and methods to produce novel analgesic drugs
Methods of forming integrated circuit packages
TRPM8 antagonists and their use in treatments
Bisphosphonate compounds
Tropoelastin derivatives
Image processing apparatus and image processing method
Systems and methods for providing a shared folder via television
  Randomly Featured Patents
Brake rotor puller
Mirror array for lithography
Shared storage device and method for updating contents of the same
Anode electrode for an electrolytic capacitor
Polymer particles and encapsulated compositions using organoborane amine complexes
Dental treatment tray
System and method for enabling multi-element bidding for influencing a position on a search result list generated by a computer network search engine
Semiconductor packaging substrate improving capability of electrostatic dissipation
Citric acid derivatives as emulsifiers for emulsion polymerization
Multipoint television conference system