Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Highly sensitive enzymatic assay method










Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.








 
 
  Recently Added Patents
Scanned image projection system employing intermediate image plane
Module-code verification layer to automatically validate user input
Polymeric compositions including their uses and methods of production
Editing device and editing method
2-aryl-4-quinazolinones and their pharmaceutical compositions
System and method for updating firmware
Optical modulation element
  Randomly Featured Patents
Headrest for alleviating whiplash injury and the use of specific polyurethane foams therein
Composite fabric, in particular for hand luggage or clothes
Anti-inflammatory agents
Memory card
Catheter including a bendable portion
Short message service cipher
Triangular rollable and collapsible boom
Compliant pad wafer chuck
Substituted oxindole derivatives, medicaments containing said derivatives and use thereof
Bed construction with reduced sagging