Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Highly sensitive enzymatic assay method










Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.








 
 
  Recently Added Patents
Normalized contextual performance metric for the assessment of fatigue-related incidents
Method and system for shared high speed cache in SAS switches
Method of requesting CQI reports
Etch resistant clearcoat
Quantum dot template for fast and simultaneous detection of different infectious agents
Assisting apparatus, method, and program for checking crosstalk noise between hierarchized modules in a semiconductor circuit
Container
  Randomly Featured Patents
Thin-film transistor and method for manufacturing the same
Co-processed starch/gum based food ingredient and method of making the same
Spout
Universal primer
Image forming apparatus with function of sorting plurality of sheaves of original paper
Digital filters for semiconductor devices
Protective cover sleeve for laptop computer screens
Poly(ethylene/alkylene) terephthalates which crystallize rapidly
Fluid handling structure, lithographic apparatus and device manufacturing method
Method for producing semiconductor device including a refractory metal pattern