Resources Contact Us Home
Browse by: INVENTOR PATENT HOLDER PATENT NUMBER DATE
 
 
Highly sensitive enzymatic assay method










Image Number 3 for United States Patent #4791057.

A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.








 
 
  Recently Added Patents
Control device with adjusting pulse width modulation function and the backlight module thereof
Electronic device, information processing method, and storage medium
Mono-body defibrillation probe
Magnetic disk and manufacturing method thereof
Clip
Hybrid interconnect scheme including aluminum metal line in low-k dielectric
Battery loading and unloading mechanism
  Randomly Featured Patents
Electrical connector and terminal structure thereof
Display
Explicit casualty control in a client/server system
Gas chromatograph techniques for on-line testing of transformer faults
Apparatus for enhancing the environmental quality of work spaces
Hybrid double hydrocyclone-gravity gas/liquid separator
Benzimidazole derivatives
Method and apparatus for reducing a computational result to the range boundaries of a signed 8-bit integer in case of overflow
Method, system, and computer program product for automatically managing components within a controlled environment
Correcting doping defects