| Patent Number |
Title Of Patent |
Date Issued |
| RE36536 |
Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
January 25, 2000 |
| In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formula ##STR1## where T is a cyclo |
| 6690399 |
Data display software for displaying assay results |
February 10, 2004 |
| A method and apparatus for displaying data presents results from a single assay across multiple wells and plates on a single screen in a format that preserves the spatial relationship between the wells on a plate and the sequential relationship between plates. Also provided to the user i |
| 6686171 |
Competitive chemiluminescent assay for cyclic nucleotide monophosphates |
February 3, 2004 |
| A competitive assay for the concentration of a cyclic nucleotide such as cyclic adenosine monophosphate combines, in a reaction chamber provided with a capture antibody, an antibody for the cAMP or other cyclic nucleotide, the sample to be assayed and a conjugate of cAMP and alkaline |
| 6602658 |
Multiple reporter gene assay |
August 5, 2003 |
| A method of measuring the activity of at least two reporter gene products in an aliquot of a sample extract is disclosed. The activities of a first and second reporter enzyme are quantified by measuring the light signal produced by degradation of a first substrate by the first reporter e |
| 6602657 |
Multiple reporter gene assay |
August 5, 2003 |
| A method of measuring the activity of at least two reporter gene products in an aliquot of a sample extract is disclosed. The activities of a first and second reporter enzyme are quantified by measuring the light signal produced by degradation of a first substrate by the first reporter e |
| 6586196 |
Multiple enzyme assays |
July 1, 2003 |
| The present invention discloses multiple enzyme assays which measure the activity of at least one endogenous enzyme in a single aliquot and a method of measuring the activity of multiple enzymes in an aliquot of a cell extract, wherein at least one of the enzymes is an endogenous enzyme. |
| 6555698 |
Chemiluminescent substrates for neuraminidase, assays for detection of neuraminidase and kits th |
April 29, 2003 |
| The present invention discloses chemiluminescent 1,2-dioxetane substrates capable of reacting with a neuraminidase to release optically detectable energy. These 1,2-dioxetanes have the general formula: ##STR1##wherein Z is ##STR2##and the variables are selected so as to induce decomp |
| 6518068 |
Luminescence detection workstation |
February 11, 2003 |
| A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples m |
| 6514717 |
Kit for conducting an assay to detect a substance using enzymatically-induced decomposition of d |
February 4, 2003 |
| A kit for detecting a first substance in a sample including a stabilized 1,2-dioxetane bearing an enzyme-labile substituent, which is destabilized and caused to decompose by contacting the 1,2-dioxetane with an enzyme under conditions which cause the enzyme to cleave the enzyme-labile |
| 6451531 |
Dioxetane labeled probes and detection assays employing the same |
September 17, 2002 |
| Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor |
| 6355441 |
Benzothiazole dioxetanes |
March 12, 2002 |
| Chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature, represented by the formulas I or II: ##STR1## |
| 6346615 |
Chemiluminescent 1,2-dioxetanes |
February 12, 2002 |
| Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is |
| 6322727 |
Kit for conducting an assay to detect a substance using enzymatically-induced decomposition of d |
November 27, 2001 |
| A kit for detecting a first substance in a sample including a stabilized 1,2-dioxetane bearing an enzyme-labile substituent, which is destabilized and caused to decompose by contacting the 1,2-dioxetane with an enzyme under conditions which cause the enzyme to cleave the enzyme-labile gr |
| 6287767 |
Chemiluminescent energy transfer assays |
September 11, 2001 |
| A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos.TM. as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioa |
| 6243980 |
Protease inhibitor assay |
June 12, 2001 |
| Heterogenous and homogenous assays are provided for the detection of protease inhibitory activity in a sample or target compound, taking advantage of the chemiluminescent characteristics of 1,2-dioxetanes. In the heterogenous assay, a peptide bearing a cleavage site for the protease |
| 6183978 |
Luciferase assay, compositions and kits for use in connection therewith |
February 6, 2001 |
| An assay for the presence of luciferase in a biological sample offers heightened sensitivity, signal intensity and persistence. The assay is sensitive down to 50 fg luciferase. The biological sample is combined with essential ingredients luciferin, ADP, myokinase and Mg.sup.++. The m |
| 6162610 |
Xanthan-ester and acridan substrates for horseradish peroxidase |
December 19, 2000 |
| Xanthan esters and acridans are substrates for horseradish peroxidase. These stable, enzymatically cleavable chemiluminescent esters are substrates for horseradish peroxidase which, together with peroxide is among the extensively used enzyme in enzyme-linked detection methods, includ |
| 6140495 |
Chemiluminescent 1,2-dioxetanes |
October 31, 2000 |
| Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is |
| 6133459 |
Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes |
October 17, 2000 |
| A method and compositions including a 1,2-dioxetane and a fluorescent compound is described. In particular, enzymatic triggering of a triggerable 1,2-dioxetane admixed with a surfactant and the fluorescent compound attached to a hydrocarbon to provide a co-surfactant in a micelle or |
| 6132956 |
Chemiluminescent 1,2-dioxetanes |
October 17, 2000 |
| Dioxetanes that can be triggered by bases are provided which can be used to detect bases in organic solvents, aqueous preparations and detect gaseous bases in the atmosphere. The dioxetanes can be used to detect the release of bases from various labels, as a means to detect the presence |
| 6124478 |
Methods of using 1,2-dioxetanes and kits therefore |
September 26, 2000 |
| Compounds having the formula: ##STR1## wherein T is a polycycloalkylidene group (e.g., adamant-2-ylidene); R is a C.sub.1-20 alkyl, aralkyl or cycloalkyl group; and Y is a fluorescent chromophore (e.g., m-phenylene), produced by reacting a compound having the formula: ##STR2# |
| 6113816 |
Kit for conducting an assay to detect a substance using enzymatically-induced decomposition of d |
September 5, 2000 |
| A kit for detecting a first substance in a sample comprising a dioxetane having the formula: ##STR1## wherein T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is H, alkyl, aryl, |
| 6107024 |
Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes |
August 22, 2000 |
| A method and compositions including a 1,2-dioxetane and a fluorescent compound is described. In particular, enzymatic triggering of a triggerable 1,2-dioxetane admixed with a surfactant and the fluorescent compound attached to a hydrocarbon to provide a co-surfactant in a micelle or |
| 6063574 |
Dioxetane labeled probes and detection assays employing the same |
May 16, 2000 |
| Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor |
| 6022964 |
Chemiluminescent 1,2-dioxetanes |
February 8, 2000 |
| Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is |
| 5994073 |
Enhancement of chemiluminescent assays |
November 30, 1999 |
| Chemiluminescent bioassays for the presence or concentration of an analyte in a sample use 1,2-dioxetanes as substrates for the enzyme of an enzyme complex that bind to the analyte. The chemiluminescence obtained from the decomposition of the dioxetane triggered by the enzyme through the |
| 5981768 |
1,2 chemiluminescent dioxetanes of improved performance |
November 9, 1999 |
| A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably s |
| 5891626 |
Method providing enhanced chemiluminescence from 1,2-dioxetanes |
April 6, 1999 |
| A method and compositions including a 1,2-dioxetane and a fluorescent compound is described. In particular, enzymatic triggering of a triggerable 1,2-dioxetane admixed with a surfactant and the fluorescent compound attached to a hydrocarbon to provide a co-surfactant in a micelle or |
| 5871938 |
Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits the |
February 16, 1999 |
| 1,2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the genera |
| 5869705 |
Enzyme-activated 1,2-dioxetanes |
February 9, 1999 |
| In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formula ##STR1## where T is a cyclo |
| 5869699 |
1,2 chemiluminescent dioxetanes of improved performance |
February 9, 1999 |
| A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably s |
| 5866389 |
Kit for conducting an assay to detect a substance using enzymatically-induced decomposition of d |
February 2, 1999 |
| A kit including a dioxetane and an enzyme reactive therewith is useful in an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with the enzym |
| 5856522 |
Method of using synthesis of 1,2-dioxetanes and kits therefore |
January 5, 1999 |
| Kits comprising 1,2-dioxetanes which can be cause to chemiluminesce by contact with an enzyme, and the enzyme, are provided for use in optically detectable assays. The assay calls for binding the enzyme to the substance to be detected in a sample, removing any unbound enzyme, and then |
| 5851771 |
Chemiluminescent 1,2-dioxetanes |
December 22, 1998 |
| Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is |
| 5849495 |
Chemiluminescent energy transfer assays |
December 15, 1998 |
| A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos.TM. as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioa |
| 5847161 |
1, 2 chemiluminescent dioxetanes of improved performance |
December 8, 1998 |
| A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably s |
| 5843681 |
Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits the |
December 1, 1998 |
| 1, 2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the g |
| 5840919 |
Chemiluminescent 1,2-dioxetanes |
November 24, 1998 |
| Novel 1,2-dioxetanes with improved chemiluminescent properties, such as signal intensity, S/N ratio, T1/2, etc. are provided by spiroadamantyl 1,2-dioxetanes, wherein the remaining carbon atom of the ring bears an alkoxy, aryloxy, or arylalkoxy substituent, and either a phenyl or nap |
| 5831102 |
Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes |
November 3, 1998 |
| Enzymatically cleavable chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature before a bond by which an enzymatically cleavable labile substituent thereof is intentionally cleaved, are disclosed. These com |
| 5827650 |
Membrane for chemiluminescent blotting applications |
October 27, 1998 |
| A new and improved polymeric membrane for use in biological assays is provided. A blotting assay employing 1,2-dioxetanes as a source of chemiluminescent employs, as an improved membrane, a polymer comprised of at least one monomer of the formula: ##STR1## The membranes reduce ba |
| 5800999 |
Dioxetane-precursor-labeled probes and detection assays employing the same |
September 1, 1998 |
| Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor |
| 5783381 |
Chemiluminescent 1,2-dioxetanes |
July 21, 1998 |
| Dioxetanes that can be triggered by bases are provided which can be used to detect bases in organic solvents, aqueous preparations and detect gaseous bases in the atmosphere. The dioxetanes can be used to detect the release of bases from various labels, as a means to detect the presence |
| 5777133 |
Synthesis of 1, 2-dioxetanes and intermediates therefor |
July 7, 1998 |
| Compounds having the formula: ##STR1## wherein T is a polycycloalkylidene group (e.g., adamant-2-ylidene); R is a C.sub.1-20 alkyl, aralkyl or cycloalkyl group; and Y is a fluorescent chromophore (eg., m-phenylene), produced by reacting a compound having the formula: ##STR2## |
| 5773628 |
1,2-dioxetane compounds with haloalkoxy groups, methods preparation and use |
June 30, 1998 |
| A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a halo |
| 5763681 |
Synthesis of stable, water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
June 9, 1998 |
| A novel synthesis of compound having the formula: ##STR1## wherein T is a stabilizing spiro-linked polycycloalkylidene group, R.sup.3 is a C.sub.1 -C.sub.20 alkyl, aralkyl or heteroatom containing group, Y is an aromatic fluorescent chromophore, and Z is a cleavable group which, |
| 5756770 |
Synthesis of stable, water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
May 26, 1998 |
| A novel synthesis of 1,2-dioxetane phosphate alkali metal salts and novel intermediates employed in this synthesis are disclosed. A hydroxyaryl enol ether alkali metal salt having the formula: ##STR1## wherein T can be an unsubstituted or substituted adamant-2'-ylidene group, R.s |
| 5753436 |
Chemiluminescent energy transfer assays |
May 19, 1998 |
| A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos.TM. as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioa |
| 5707559 |
Chemiluminescent 1,2-dioxetane compounds |
January 13, 1998 |
| Novel light producing 1,2-dioxetanes are described of the formula ##STR1## wherein ArOX is an aryl ring substituted with an X oxy group and A are passive organic groups which allow the 1,2-dioxetane to produce light when triggered by removing X. X is a chemically labile group which i |
| 5679803 |
1,2 chemiluminescent dioxetanes of improved performance |
October 21, 1997 |
| A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably s |
| 5679802 |
1,2-dioxetanes useful in chemiluminescent immunoassays |
October 21, 1997 |
| A compound of the formula ##STR1## where n, Q, and Z are as defined useful in chemiluminescent immunoassays. |
