| Patent Number |
Title Of Patent |
Date Issued |
| 7225079 |
System and method for automated matched ion polynucleotide chromatography |
May 29, 2007 |
| In an extensive Matched Ion Polynucleotide Chromatography (MIPC) system and method, and the computer programs or software associated therewith, the system provides automated options for sample selection, mobile phase gradient selection and control, column and mobile phase temperature |
| 7169298 |
Method and apparatus for separating polynucleotides using monolithic capillary columns |
January 30, 2007 |
| Methods and devices based on capillary monolithic columns, preferably consisting of an underivatized poly(styrene-divinylbenzene) monolith, for separating a mixture of polynucleotides by ion pair-reverse phase-high performance chromatography (IP-RP-HPLC). In various aspects of the method |
| 7138518 |
Liquid chromatographic separation of polynucleotides |
November 21, 2006 |
| In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivale |
| 7135289 |
Methods and compositions for mutation analysis of polynucleotides by liquid chromatography |
November 14, 2006 |
| Methods, compositions, and kits for separating heteroduplex and homoduplex DNA molecules in a test mixture by temperature-compression denaturing high performance liquid chromatography (tcDHPLC). The method includes use of nitrogen-containing additives in the mobile phase that allow detec |
| 7129075 |
Isolated CEL II endonuclease |
October 31, 2006 |
| The present invention relates to the isolation and characterization of CEL I and CEL II endonuclease proteins. Methods and kits for identifying mismatches in double-stranded DNA are also provided. |
| 6894048 |
Sulfur containing dihydrophthalazine antagonists of excitatory amino acid receptors |
May 17, 2005 |
| Substituted dihydrophthalazine sulfur containing compositions are provided which are active as non-NMDA ionotropic excitatory amino acid (EAA) receptor antagonists. The compositions are useful for treating disorders associated with excessive activation of the non-NMDA subtype of the |
| 6887867 |
5H-2,3-benzodiazepine antagonists of excitatory amino acid receptors |
May 3, 2005 |
| Substituted benzodiazepine compositions are provided which are active as non-NMDA ionotropic excitatory amino acid (EAA) receptor antagonists. The compounds are generally 7- or 8-mono substituted 5H-2,3-benzodiazepines. The compositions are useful for treating disorders associated with |
| 6838242 |
Modifying double stranded DNA to enhance separations by matched ion polynucleotide chromatograph |
January 4, 2005 |
| Covalently bound non-polar tags are used to increase the retention times of double stranded polynucleotides on Matched Ion Polynucleotide Chromatography (MIPC) columns. In doing so, separations of DNA mixture components is improved. Additionally, when the non-polar tags are fluoropho |
| 6737022 |
Fragment collector apparatus |
May 18, 2004 |
| A system for separating an aqueous stream of mixed polynucleotides into a series of length-based polynucleotide fractions and collecting one or more of the length-based polynucleotide fractions into separate containers. The system comprises a separation column containing separation media |
| 6703390 |
Sulfur containing dihydrophthalazine antagonists of excitatory amino acid receptors |
March 9, 2004 |
| Substituted dihydrophthalazine sulfur containing compositions are provided which are active as non-NMDA ionotropic excitatory amino acid (EAA) receptor antagonists. The compositions are useful for treating disorders associated with excessive activation of the non-NMDA subtype of the |
| 6652745 |
Column for DNA separation by matched ion polynucleotide chromatography |
November 25, 2003 |
| An improved separation column and method for separating a mixture of double stranded DNA fragments by Matched Ion Polynucleotide Chromatography. The cylindrical column has an ID greater than about 5 mm and contains polymer beads. The beads have an average diameter of 1 to 100 microns and |
| 6642374 |
Process for separation of polynucleotide fragments |
November 4, 2003 |
| A batch process for obtaining polynucleotide fragments, such as dsDNA, having a selected size from a mixture of polynucleotide fragments including the steps of a) applying a solution of the mixture of polynucleotide fragments and a counterion agent to a binding medium having a hydrop |
| 6579459 |
System and method for performing polynucleotide separations using liquid chromatography |
June 17, 2003 |
| Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatog |
| 6576133 |
Method and system for RNA analysis by matched ion polynucleotide chromatography |
June 10, 2003 |
| A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a polymeric separation medium having non-polar surfaces and eluting the RNA molecules with a mobile phase which includes |
| 6524480 |
Non-polar media for polynucleotide separations |
February 25, 2003 |
| Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped |
| 6521411 |
Method and system for the preparation of cDNA |
February 18, 2003 |
| In one aspect, the present invention concerns an improved method for the preparation of cDNA libraries. Preferred embodiments of the invention include methods and systems capable of generating cDNA libraries enriched for high molecular weight cDNA inserts. The method generally entails th |
| 6521123 |
Polynucleotide separations on polymeric separation media |
February 18, 2003 |
| Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surf |
| 6503397 |
Non-polar media for polynucleotide separations |
January 7, 2003 |
| Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped |
| 6491821 |
System and method for performing polynucleotide separations using liquid chromatography |
December 10, 2002 |
| Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatog |
| 6488855 |
Non-polar media for polynucleotide separations |
December 3, 2002 |
| Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped |
| 6485648 |
MIPC column cleaning system and process |
November 26, 2002 |
| An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation solution supply means, and a separation solution conduit commu |
| 6482317 |
Polynucleotide separations on polymeric separation media |
November 19, 2002 |
| Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surf |
| 6475388 |
Method and system for RNA analysis by matched ion polynucleotide chromatography |
November 5, 2002 |
| A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a reverse phase column containing polymeric beads and eluting, preferably under denaturing conditions, the RNA molecules |
| 6471866 |
Process for performing polynucleotide separations |
October 29, 2002 |
| The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non |
| 6461819 |
Analysis of nicked DNA by matched ion polynucleotide chromatography under denaturing conditions |
October 8, 2002 |
| The present invention provides a method for calibrating a MIPC column wherein the calibration relates to the determination of the organic solvent component in the mobile phase required to elute dsDNA fragments of different base pair lengths at specific retention times. Since a MIPC c |
| 6455692 |
Method of concentrating polynucleotides using MIPC |
September 24, 2002 |
| The present invention is directed to improved methods for detection of mutations in DNA using Denaturing Matched Ion Polynucleotide Chromatography (DMIPC). The invention includes the following aspects: analysis of PCR amplification products to identify factors that affect PCR replica |
| 6419824 |
Apparatus and method for separating and purifying polynucleotides |
July 16, 2002 |
| The disclosure describes an ambient or low pressure device for separating polynucleotide fragments from a mixture of polynucleotide fragments comprises a tube having an upper solution input chamber, a lower eluant receiving chamber, and a fixed unit of separation media supported therein. |
| 6372142 |
Column for DNA separation by matched ion polynucleotide chromatography |
April 16, 2002 |
| An improved separation column and method for separating a mixture of double stranded DNA fragments by Matched Ion Polynucleotide Chromatography. The cylindrical column has an ID greater than about 5 mm and contains polymer beads. The beads have an average diameter of 1 to 100 microns and |
| 6372130 |
Non-polar media for polynucleotide separations |
April 16, 2002 |
| Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped |
| 6361672 |
Multiple laser diode electromagnetic radiation source in multiple electrophoresis channel system |
March 26, 2002 |
| The present invention is a multiple channel electrophoresis system which includes a multiple laser diode illumination source. The multiple laser diode illumination source has a plurality of individual laser diodes, each of which is situated so as to provide independent fluorescence induc |
| 6355791 |
Polynucleotide separations on polymeric separation media |
March 12, 2002 |
| Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surface of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surfa |
| 6355417 |
Band array display of polynucleotide separations |
March 12, 2002 |
| A method and apparatus for representing double stranded nucleic acid fragments which have been separated by a chromatographic process as an array of bands which can be accurately quantified, optimized and stored. Using, for example, a Matched Ion Polynucleotide Chromatography (MIPC) |
| 6355165 |
MIPC chromatographic apparatus with improved temperature control |
March 12, 2002 |
| A liquid chromatography apparatus with stationary and mobile phase temperature controls suitable for polynucleotide separations by MIPC and DMIPC processes. The apparatus includes heater means with a temperature control system; a matched ion polynucleotide chromatography separation c |
| 6342161 |
Method for high resolution liquid chromatographic separation of double-stranded DNA |
January 29, 2002 |
| Mixtures of dsDNA fragments are separated by Matched Ion Polynucleotide Chromatography (MIPC) using an isocratic mobile phase to elute polynucleic acid from an MIPC column. The use of isocratic elution conditions provides a marked improvement in the separation of dsDNA fragments compared |
| 6309549 |
Polynucleotide separations on polymeric separation |
October 30, 2001 |
| Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surf |
| 6287822 |
Mutation detection method |
September 11, 2001 |
| The present invention is directed to improved methods for detection of mutations in DNA using Denaturing Matched Ion Polynucleotide Chromatography (DMIPC). The invention includes the following aspects: analysis of PCR amplification products to identify factors that affect PCR replica |
| 6265168 |
Apparatus and method for separating and purifying polynucleotides |
July 24, 2001 |
| A method for removing a target DNA fragment having a predetermined base-pair length from a mixture of DNA fragments comprises the following steps. A mixture of DNA fragments which may contain the target DNA fragments is applied to a separation column containing media having a nonpola |
| 6258264 |
Non-polar media for polynucleotide separations |
July 10, 2001 |
| Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped |
| 6251272 |
System and column for performing polynucleotide separations using liquid chromatography |
June 26, 2001 |
| Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatog |
| 6218153 |
Target DNA amplification by MIPC and PCR |
April 17, 2001 |
| A method for detecting a putative mutant DNA in a sample of DNA includes the steps of amplifying the sample of DNA using PCR; hybridizing the amplified sample to form a mixture of homoduplexes and heteroduplexes; separating the mixture into fractions by Denaturing Matched Ion Polynuc |
| 6210885 |
Modifying double stranded DNA to enhance separations by matched ion polynucleotide chromatograph |
April 3, 2001 |
| Covalently bound non-polar tags are used to increase the retention times of double stranded polynucleotides on Matched Ion Polynucleotide Chromatography (MIPC) columns. In doing so, separations of DNA mixture components is improved. Additionally, when the non-polar tags are fluoropho |
| 6187539 |
Analysis of nicked DNA by matched ion polynucleotide chromatography |
February 13, 2001 |
| The present invention provides a method for calibrating a MIPC column wherein the calibration relates to the determination of the organic solvent component in the mobile phase required to elute dsDNA fragments of different base pair lengths at specific retention times. Since a MIPC c |
| 6177559 |
Process for separation of polynucleotide fragments |
January 23, 2001 |
| A batch process for obtaining polynucleotide fragments, such as dsDNA, having a selected size from a mixture of polynucleotide fragments including the steps of a) applying a solution of the mixture of polynucleotide fragments and a counterion agent to a binding medium having a hydrop |
| 6174441 |
Method for performing polynucleotide separations using liquid chromatography |
January 16, 2001 |
| Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatog |
| 6156206 |
Process for performing polynucleotide separations |
December 5, 2000 |
| The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non |
| 6136195 |
MIPC column cleaning system and process |
October 24, 2000 |
| An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation solution supply means, and a separation solution conduit commu |
| 6103112 |
MIPC chromatographic apparatus with improved temperature control |
August 15, 2000 |
| A liquid chromatography apparatus with stationary and mobile phase temperature controls suitable for polynucleotide separations by MIPC and DMIPC processes. The apparatus includes heater means with a temperature control system; a matched ion polynucleotide chromatography separation c |
| 6074880 |
Sample analyte containing solution fraction collection system, and method of use |
June 13, 2000 |
| Disclosed is a system and method which allows user controlled formation, and ejection of droplets of sample analyte containing solutions, thereby enabling precise user directed fractionalization and distributed collection of sample analytes. |
| 6066258 |
Polynucleotide separations on polymeric separation media |
May 23, 2000 |
| Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surf |
| 6056877 |
Non-polar media for polynucleotide separations |
May 2, 2000 |
| Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped |
