| Patent Number |
Title Of Patent |
Date Issued |
| RE38723 |
Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
April 12, 2005 |
| The subject invention provides substrates useful for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process inv |
| 7446202 |
Cyanine dye compounds |
November 4, 2008 |
| Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula ##STR00001## wherein W forms one or two fused 5- or 6-membered aromatic rings, a has a value of 0 or 1, n |
| 7445894 |
Compositions and methods for detection and isolation of phosphorylated molecules |
November 4, 2008 |
| The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being |
| 7129346 |
Crown ether derivatives |
October 31, 2006 |
| The invention describes crown ether chelators, including crown ethers having the formula ##STR00001## and aza-substituted and thia-substituted analogs thereof. These crown ethers may be substituted by a dye moiety, a chemically reactive group, a conjugated substance, or a combination |
| 7102005 |
Compositions and methods for detection and isolation of phosphorylated molecules |
September 5, 2006 |
| The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being |
| 6977305 |
Modified carbocyanine dyes and their conjugates |
December 20, 2005 |
| Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior |
| 6974873 |
Modified carbocyanine dyes and their conjugates |
December 13, 2005 |
| Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior |
| 6972326 |
Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes |
December 6, 2005 |
| The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or d |
| 6967251 |
Reagents for labeling biomolecules having aldehyde or ketone moieties |
November 22, 2005 |
| Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: ##STR1##wherein Q is carbonyl, thiocarbonyl, or sulfonyl, |
| 6716979 |
Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings |
April 6, 2004 |
| The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the |
| 6664047 |
Aza-benzazolium containing cyanine dyes |
December 16, 2003 |
| Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually |
| 6579718 |
Carbazolylvinyl dye protein stains |
June 17, 2003 |
| The present invention describes a variety of substituted and unsubstituted carbazolylvinyl dyes and their use for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by |
| 6562632 |
Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
May 13, 2003 |
| The invention describes reactive dyes having an alkyl spacer attached via a sulfonamide bond to a sulforhodamine 101 fluorophore, and a variety of useful conjugates prepared therefrom. The increased length of the covalent linkage due to the alkyl spacer results in dye-conjugates having a |
| 6399392 |
Xanthene dyes and their application as luminescence quenching compounds |
June 4, 2002 |
| The quenching compounds of the invention are nitrogen-substituted xanthenes that are substituted by one or more aromatic or heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable fluorescence and efficiently quench a broad spectrum of |
| 6329205 |
Detection method using luminescent europium-based protein stains |
December 11, 2001 |
| The invention relates to the staining of amine-containing polymers, including including peptides, polypeptides, and proteins, in gels and on solid supports, using complexes of europium (3+). |
| 6323337 |
Quenching oligonucleotides |
November 27, 2001 |
| The invention relates to oligonucleotides labeled with an energy transfer acceptor useful in conjunction with fluorescent nucleic acid stains. The resulting oligonucleotides are useful for decreasing background fluorescence during amplification assays and in ligation assays, and for |
| 6323186 |
Phosphate-bound polyazaindacene derivatives of nucleotides |
November 27, 2001 |
| The invention relates to dipyrrometheneboron difluoride-substituted nucleotides substituted by a dipyrrometheneboron difluoride label on a phosphate moiety. The nucleotides of the invention are useful in fluorescence assays, including protein binding assays and assays for hydrolase e |
| 6316267 |
Luminescent protein stains an their method of use |
November 13, 2001 |
| The invention relates to the staining of poly(amino acids), including peptides, polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals. |
| 6291203 |
Cyanine dyes that stain cells and mitochondria |
September 18, 2001 |
| The present invention relates to the use of positively-charged unsymmetrical cyanine dyes, including novel unsymmetrical cyanine dyes, to stain cells and selectively stain intracellular organelles, with particular advantages in staining mitochondria. The dyes are generally weakly flu |
| 6265179 |
Detection of phosphate using coupled enzymatic reactions |
July 24, 2001 |
| Inorganic phosphate may be detected and optionally quantified via the coupling of a phosphate-dependent enzymatic reaction with an enzyme system that generates hydrogen peroxide in the presence of a chromogenic or fluorogenic peroxidase substrate. Phosphate consuming or phosphate-pro |
| 6229055 |
Synthesis of fluorinated xanthene derivatives |
May 8, 2001 |
| Facile syntheses for fluorinated resorcinol and aminophenol derivatives are provided that yield isomer-free products in good yield. These novel methods use generally available precursors and standard laboratory reagents and equipment to reproducibly produce these synthetically useful |
| 6162931 |
Fluorinated xanthene derivatives |
December 19, 2000 |
| The family of dyes of the invention are fluoresceins and rhodols that are directly substituted on one or more aromatic carbons by fluorine. These fluorine-substituted fluorescent dyes possess greater photostability and have lower sensitivity to pH changes in the physiological range of 6- |
| 6130101 |
Sulfonated xanthene derivatives |
October 10, 2000 |
| The present invention describes xanthene dyes, including rhodamines, rhodols and fluoresceins that are substituted one or more times by a sulfonic acid or a salt of a sulfonic acid. The dyes of the invention, including chemically reactive dyes and dye-conjugates are useful as fluores |
| 6013802 |
Fluorescent conjugates of metal-chelating nitrogen heterocycles |
January 11, 2000 |
| The present invention describes a family of fluorescent indicators for metal cations. The indicators are fluorophore conjugates of pyridyl-based metal ion chelators. The indicators are very sensitive detection as quantification reagents for a variety of metals, in a variety of oxidation |
| 6005113 |
Long wavelength dyes for infrared tracing |
December 21, 1999 |
| The invention relates to fluorescent dyes that are substituted or unsubstituted derivatives of 1-(isoindolyl)methylene-isoindole that are bound through both isoindole nitrogens to a boron difluoride moiety, forming a fluorescent dibenzopyrrometheneboron difluoride compound ##STR1## |
| 6004536 |
Lipophilic cyanine dyes with enchanced aqueous solubilty |
December 21, 1999 |
| The present invention relates to a family of cyanine dyes possessing lipophilic alkyl chains and either one or more reactive functional groups, bromo or chloro, or phenyl, sulfophenyl or polysulfophenyl substituents or combinations thereof. The dyes of the invention are useful for staini |
| 5888829 |
Photolabile caged ionophores and method of using in a membrane separation process |
March 30, 1999 |
| The present invention describes a family of photolabile caged ionophores. The compounds of the present invention are photolytically cleavable esters of nigericin, ionomycin, A-23187, 4-Br-A-23187 and monensin. The photolysis of the present compounds allows the release of the free ion |
| 5872243 |
Caged nucleotides |
February 16, 1999 |
| The present invention describes a family of photolabile caged nucleotides, including cyclic nucleotides. The compounds of the present invention are caged analogs and derivatives of NAD.sup.+, NADH, NADP, NADPH, NAADP and cADPR. The photolysis of the present compounds allows the release o |
| 5869689 |
Stains for acidic organelles |
February 9, 1999 |
| Dipyrrometheneboron difluoride dyes possessing a covalently attached basic amine moiety are described that have utility for staining acidic organelles. Samples comprising isolated acidic organelles, or a cell or cells containing acidic organelles, are stained by preparing an aqueous |
| 5863753 |
Chemically reactive unsymmetrical cyanine dyes and their conjugates |
January 26, 1999 |
| The invention comprises cyanine dyes, in particular chemically reactive dyes, conjugates of reactive cyanine dyes, the non-covalent complexes of nucleic acids with the dyes and dye-conjugates of the invention, and a method of forming a nucleic acid complex with the dyes and dye-conjugate |
| 5846737 |
Conjugates of sulforhodamine fluorophores with enhanced fluorescence |
December 8, 1998 |
| The invention describes useful conjugates of sulforhodamine, wherein the conjugated substance and the fluorophore are separated by an alkanoic acid spacer that is attached to the fluorophore via a sulfonamide bond. The increased length of the covalent linkage due to the spacer results in |
| 5830912 |
Derivatives of 6,8-difluoro-7-hydroxycoumarin |
November 3, 1998 |
| The present invention comprises 6,8-difluoro-7-hydroxycoumarins and derivatives of 6,8-difluoro-7-hydroxycoumarins, including reactive dyes, dye-conjugates and enzyme substrates. These fluorine-substituted fluorescent dyes typically possess greater photostability and lower pH sensiti |
| 5798276 |
Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
August 25, 1998 |
| The invention describes reactive dyes having an alkyl spacer attached via a sulfonamide bond to a sulforhodamine 101 fluorophore, and a variety of useful conjugates prepared therefrom. The increased length of the covalent linkage due to the alkyl spacer results in dye-conjugates having a |
| 5786219 |
Microspheres with fluorescent spherical zones |
July 28, 1998 |
| The invention describes novel fluorescently labeled microspheres, where the microspheres possess at least one internal fluorescent spherical zone. The invention also describes the method of preparing the novel microspheres, the method of calibrating microscopy instrumentation using the n |
| 5773227 |
Bifunctional chelating polysaccharides |
June 30, 1998 |
| This invention describes bifunctional polysaccharides conjugated to both a chelating group suitable for the selective complexation of metal cations, and a targeting peptide specific for a cellular substructure. These bifunctional polysaccharides are primarily useful for the regulation, |
| 5723218 |
Dipyrrometheneboron difluoride labeled flourescent microparticles |
March 3, 1998 |
| The invention is a novel fluorescently labeled microparticle, where the microparticle internally incorporates at least one dipyrrometheneboron difluoride dye. Appropriate selection of substituents results in dipyrrometheneboron difluoride derivatives that, when incorporated into poly |
| 5719031 |
Dye labeled polymers as reagents for measuring polymer degradation |
February 17, 1998 |
| This invention relates to polymers labeled with fluorescent dye to the point that significant fluorescence quenching occurs, such that degradation of the polymer results in fluorescence enhancement. The resulting fluorescence enhancement is useful for measuring the degradation of suc |
| 5696157 |
Sulfonated derivatives of 7-aminocoumarin |
December 9, 1997 |
| The present invention describes 7-aminocoumarin dyes that are substituted one or more times at the 3-, 6- and/or 8-positions by a sulfonic acid or a salt of a sulfonic acid, said dyes being useful as fluorescent probes or in the preparation of enzyme substrates, caged probes, or adducts |
| 5686261 |
Xanthylium dyes that are well retained in mitochondria |
November 11, 1997 |
| The present invention describes a method of staining mitochondria, and analyzing mitochondrial function, using a class of fluorescent substituted 3',6'-diaminoxanthenes and their reduced analogs 3',6'-diaminodihydroxanthenes, which are oxidized to the fluorescent form of the dye in s |
| 5658751 |
Substituted unsymmetrical cyanine dyes with selected permeability |
August 19, 1997 |
| The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a pyridinium or quinolinium ring system having at least one substituent on th |
| 5656449 |
Neutral unsymmetrical cyanine dyes |
August 12, 1997 |
| The invention describes the preparation and use of fluorescent stains for nucleic acids derived from neutral unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked to a methine bridge to a pyridine or quinoline ring system. The fluorescence characteristics of |
| 5648270 |
Methods of sensing with fluorescent conjugates of metal-chelating nitrogen heterocycles |
July 15, 1997 |
| The present invention describes the use of a family of fluorescent indicators for metal cations. The indicators are fluorophore conjugates of pyridyl-based metal ion chelators. The indicators are very sensitive detection as quantification reagents for a variety of metals, in a variety |
| 5635608 |
.alpha.-carboxy caged compounds |
June 3, 1997 |
| The invention relates to caged compounds for the study of biological processes, where the caged compound has a photoremoveable .alpha.-carboxy-substituted o-nitrobenzyl group. Covalent attachment of the substituted o-nitrobenzyl to a parent compound yields a caged compound with biolo |
| 5616502 |
Non-specific protein staining using merocyanine dyes |
April 1, 1997 |
| The present invention describes the use of a variety of merocyanine dyes and substituted merocyanine dyes for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by their |
| 5582977 |
Dimers of unsymmetrical cyanine dyes |
December 10, 1996 |
| The invention relates to dimers of unsymmetrical cyanine dyes, typically dimers of benzthiazole or benzoxazole derivatives, that exhibit enhanced fluorescence on binding with DNA or RNA, The dimers generally have the formula: ##STR1## where R.sup.1 and R.sup.2, which may be the s |
| 5576424 |
Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
November 19, 1996 |
| The subject invention provides substrates useful for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process inv |
| 5573909 |
Fluorescent labeling using microparticles with controllable stokes shift |
November 12, 1996 |
| The invention relates to methods for labeling or detecting one or more target materials using surface coated fluorescent microparticles with unique characteristics. The unique microparticles used to practice the invention have at least two components: an external substance or coating |
| 5545535 |
Fluorescent assay for bacterial gram reaction |
August 13, 1996 |
| The invention relates to a method of analyzing a sample thought to contain bacteria using an aqueous solution comprising one or more fluorescent dyes: a fluorescent dye of formula I, a fluorescent dye of formula II, a fluorescent dye of formula III, and a fluorescent dye of formula IV. E |
| 5534416 |
Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
July 9, 1996 |
| The invention relates to a method of analyzing the viability of a sample of cells using an aqueous solution comprising two fluorescent dyes. Dye I has the formula: ##STR1## where R.sup.2 is C.sub.1-6 alkyl; Z.sup.- is a biologically compatible counterion;X is O; S; Se; or NR.sup. |
| 5516864 |
Fluorescent ion-selective diaryldiaza crown ether conjugates |
May 14, 1996 |
| This invention describes novel sensors for ions that are based on the combination of xanthylium-based dyes with metal-binding N,N'-diaryldiaza crown ethers. These sensors are primarily useful for detection and quantitation of alkali-metal ions in aqueous solution. Binding of the ion |
