| Patent Number |
Title Of Patent |
Date Issued |
| RE38416 |
Cross-linking oligonucleotides |
February 3, 2004 |
| This invention is directed to novel substituted nucleotide bases with a crosslinking arm which accomplish crosslinking between specific sites on adjoining strands of oligonucleotides a oligodeoxynucleotides. The invention is also directed to oligonucleotides comprising at least one of |
| 7381818 |
Fluorescent probes containing 5'-minor groove binder, fluorophore and quenching moieties and met |
June 3, 2008 |
| Minor groove binder-oligonucleotide probes are provided along with methods for their use wherein the probes have an attached fluorophore which, in an unhybridized form exhibits very low background signal. |
| 7368549 |
T.sub.m leveling compositions |
May 6, 2008 |
| Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups. |
| 7348146 |
Single nucleotide polymorphism analysis of highly polymorphic target sequences |
March 25, 2008 |
| Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked. |
| 7319022 |
Amplification methods |
January 15, 2008 |
| Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe. |
| 7297495 |
Hybridization-triggered fluorescent detection of nucleic acids |
November 20, 2007 |
| Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence prop |
| 7252940 |
Abasic site endonuclease assay |
August 7, 2007 |
| The present invention provides a novel method for detection and/or genotyping of nucleic acids that utilizes the specificity of an AP endonuclease. In addition, the present invention provides a novel method for nucleic acid amplification. |
| 7205105 |
Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR an |
April 17, 2007 |
| Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5' nuclease digestion. |
| 7112684 |
Compounds and methods for fluorescent labeling |
September 26, 2006 |
| Dye reagents useful in labeling biological materials are provided along with methods for their use. |
| 7045610 |
Modified oligonucleotides for mismatch discrimination |
May 16, 2006 |
| Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups. |
| 6972339 |
Compounds and methods for fluorescent labeling |
December 6, 2005 |
| Dye reagents useful in labeling biological materials are provided along with methods for their use. |
| 6972328 |
Non-aggregating non-quenching oligomer comprising nucelotide analogs, method of synthesis and us |
December 6, 2005 |
| The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced |
| 6962991 |
Process for the synthesis of pyrazolopyrimidines |
November 8, 2005 |
| The present invention provides a nucleoside comprising a pyrazolopyrimidine base and a process for producing the same. In particular, the processes of the present invention comprises using a halogenated pyrazolopyrimidine base and removing the halogen after the base is coupled to a sugar |
| 6951930 |
Hybridization-triggered fluorescent detection of nucleic acids |
October 4, 2005 |
| Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properti |
| 6884584 |
Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove bind |
April 26, 2005 |
| Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI.sub.3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI.sub.3 group resides in the mino |
| 6790945 |
Fluorescent quenching detection reagents and methods |
September 14, 2004 |
| Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group. |
| 6699975 |
Fluorescent quenching detection reagents and methods |
March 2, 2004 |
| Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about 300 to about 800 nm, and or where the quencher includes a substituted 4-(phenyldiazenyl)phenylamine structure provide improved signal to noise ratios and othe |
| 6683173 |
Tm leveling methods |
January 27, 2004 |
| Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups. |
| 6660845 |
Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis a |
December 9, 2003 |
| The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced |
| 6653473 |
Fluorescent quenching detection reagents and methods |
November 25, 2003 |
| Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group. |
| 6548652 |
Attachment of oligonucleotides to solid supports through schiff base type linkages for capture a |
April 15, 2003 |
| Derivatized oligonucleotides (ODNs) are coupled to a solid support in improved yield resulting in a high density of coupled oligonucleotide per surface unit of the support, through a Schiff base type bond formed between an NH.sub.2 group attached either to the solid support or to the |
| 6486308 |
Covalently linked oligonucleotide minor groove binder conjugates |
November 26, 2002 |
| Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids thereof. The covalently bound oligonucleotide minor groove binder conjugates strogly b |
| 6472153 |
Hybridization-triggered fluorescent detection of nucleic acids |
October 29, 2002 |
| Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properti |
| 6441159 |
Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture a |
August 27, 2002 |
| Derivatized oligonucleotides (ODNs) are coupled to a solid support in improved yield resulting in a high density of coupled oligonucleotide per surface unit of the support through a Schiff base type bond formed between an NH.sub.2 group attached either to the solid support or to the ODN |
| 6426408 |
Covalently linked oligonucleotide minor groove binder conjugates |
July 30, 2002 |
| Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids thereof. The covalently bound oligonucleotide minor groove binder conjugates strogly b |
| 6339147 |
Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture a |
January 15, 2002 |
| Derivatized oligonucleotides (ODNs) are coupled to a solid support in improved yield resulting in a high density of coupled oligonucleotide per surface unit of the support, through a Schiff base type bond formed between an NH.sub.2 group attached either to the solid support or to the |
