| Patent Number |
Title Of Patent |
Date Issued |
| RE39337 |
Nude mouse model for human neoplastic disease |
October 10, 2006 |
| A nude mouse model for human neoplastic diseases having histologically intact human neoplastic tissue transplanted onto an organ of the mouse which corresponds to the human organ from which the tissue is obtained. |
| 7329516 |
Methods for increasing protein polyethylene glycol (PEG) conjugation |
February 12, 2008 |
| The present invention relates to highly conjugated proteins and methods for making such proteins. In particular, the present invention relates to methods for linking additional sites to a protein for conjugation with activated polyethylene glycol (PEG) linkers, without denaturing the |
| 7264819 |
Lyase treatment for P. carinii |
September 4, 2007 |
| Infection by P. carinii can be treated by administering methioninase optionally in combination with additional therapeutic agents, such as antibiotics. |
| 7252941 |
Expression profiling based on histocultures |
August 7, 2007 |
| Methods of obtaining faithful expression libraries from tissue samples comprise extraction of RNA from intact tissue cultured in three-dimensional sponge-gel based histocultures. |
| 7067496 |
Methods for introducing genes into mammalian subjects |
June 27, 2006 |
| Methods to obtain genetic modifications of cells in histoculture are described. Modification is assisted by treating the histoculture with collagenase prior to contacting the histoculture with the delivery vehicle for the desired gene. Hair follicles and other organized tissues can b |
| 6927038 |
Total cysteine assay |
August 9, 2005 |
| A method to determine a total cysteine in biological fluids utilizes similarly treated portions of the fluid with a homocysteinase and a non-specific desulfurase. |
| 6916793 |
Selenium-containing pro-drugs for cancer therapy |
July 12, 2005 |
| Methods for inhibiting the growth of tumor cells by combination treatment with a selenium-containing prodrug and an enzyme for which it is a substrate are described. The treatment also enhances the effect of anti-tumor agents. |
| 6905831 |
Real time measurement of cellular responses |
June 14, 2005 |
| Living cells can be stably modified to emit different colors from the cytoplasm and nucleus, thus permitting analysis of the status of said cells and the effect of agents on said cells either by visual or instrumentally-aided observation. These observations may be made, if desired, i |
| 6844326 |
Treatment of alopecia |
January 18, 2005 |
| A composition and method for treating and monitoring alopecia by altering hair follicle cells to produce a cell cycle inhibitor is disclosed. |
| 6759038 |
Metastasis models using green fluorescent protein (GFP) as a marker |
July 6, 2004 |
| A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is dis |
| 6733776 |
Method for promoting hair growth |
May 11, 2004 |
| The invention provides a method to provide hair growth by stimulating extrusion of hair from a hair follicle which employs a liposomal formulation of an active ingredient which stimulates extrusion when taken up by the hair follicles. |
| 6713273 |
High expression and production of high specific activity recombinant S-adenosyl homocysteinase ( |
March 30, 2004 |
| The invention provides novel methods relating to SAM detection and production as well as a novel SAHH enzymatic activity for use in such methods. Additional methods, compositions, and kits relating to the novel SAHH are also provided. |
| 6653278 |
Selenium-containing pro-drugs for cancer therapy |
November 25, 2003 |
| Methods for inhibiting the growth of tumor cells by combination treatment with a selenium-containing prodrug and an enzyme for which it is a substrate are described. The treatment also enhances the effect of anti-tumor agents. |
| 6653092 |
Method to determine hydrogen peroxide |
November 25, 2003 |
| A method to detect and measure the concentration of hydrogen peroxide comprises adding reagents consisting essentially of peroxidase and a dialkyl phenylenediamine and detecting any color generated by the interaction of hydrogen peroxide with these reagents. |
| 6649159 |
Whole-body optical imaging of gene expression and uses thereof |
November 18, 2003 |
| The invention relates to the whole-body external optical imaging of gene expression. Specifically, methods for whole-body external optical imaging of gene expression and methods for evaluating a candidate protocol or drug for treating diseases or disorders using a fluorophore operatively |
| 6632430 |
Modulators of methylation for control of bacterial virulence |
October 14, 2003 |
| Compositions and methods which ameliorate the virulence of bacterial infection are described wherein the active ingredient modulates transmethylation reactions in bacterial cells. Particularly useful compounds are inhibitors of S-adenosyl methionine synthetase (SAMS), of S-adenosyl h |
| 6562586 |
In vitro model for HIV and other viral diseases |
May 13, 2003 |
| An in vitro model system for viral infection is comprised of a tissue block from adult tonsil or lymph node supported on a matrix which is flexible and porous and wherin the supported tissue block is cultured in a medium whose surface is congruent with the tissue block/matrix interface. |
| 6551772 |
Model for viral infection and immune response |
April 22, 2003 |
| An in vitro model system for viral infection and for immune response is comprised of a tissue block from adult tonsil or lymph node supported on a matrix which is flexible and porous and wherein the supported tissue block is cultured in a medium whose surface is congruent with the tissue |
| 6524571 |
Methioninase gene therapy for tumor treatment |
February 25, 2003 |
| A depletion method to inhibit tumor growth includes introducing a viral expression system capable of expressing methioninase or a fusion protein containing methioninase into a tumor contained in a vertebrate subject or cells thereof. The fusion protein may contain a fluorescent protein t |
| 6468762 |
High specificity homocysteinases |
October 22, 2002 |
| Homocysteinase enzymes are disclosed which have sufficient specificity for homocysteine, as compared to cysteine that hydrogen sulfide can be used as a measure of homocysteine in a biological fluid even in the presence of substantial amounts of cysteine, exceeding the level of homocystei |
| 6461851 |
High expression modules containing two or more tandem copies of a methioninase encoding sequence |
October 8, 2002 |
| The construction of multicopy expression systems for methioninase are disclosed. The higher expression systems employ a promoter operably linked to two or more copies of a tandemly repeated methioninase encoding sequence. Such multicopy expression systems were found to produce unexpe |
| 6448446 |
Synthesis of N,N-disubstituted-p-phenylenediamine |
September 10, 2002 |
| An improved synthesis of N,N,-disubstituted-p-phenylene-diamine (DSPDA) preferably di-n-butyl-p-phenylenediamine is disclosed. The improvement rests on the discovery that DSPDA and its salts are extremely sensitive to oxygen so that once the product DSPDA or its salt is formed, oxygen mu |
| 6426194 |
Homogeneous enzymatic assay for vitamin B6 and improvements in H2S detection |
July 30, 2002 |
| Enzymatic methods to determine the concentration of pyridoxal 5'-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular disease. The assay can be a homogeneous assay using the ability of PLP to function as a co-enzyme |
| 6399380 |
Native-state histoculturing skin extracellular support |
June 4, 2002 |
| Methods and compositions for a native-state histoculturing system for skin are disclosed. Skin samples are placed on an extracellular support matrix immersed in a medium whereby the internal surface of the skin is adjacent to the matrix and the external surface is exposed above the surfa |
| 6372489 |
Method and model for hair pigmentation |
April 16, 2002 |
| A composition and method for treating disorders related to tyrosinase gene expression and melanin biosynthesis is disclosed. The composition comprises a tyrosinase encoding nucleotide sequence and an ORF-438 encoding nucleotide sequence derived from Streptomyces, adapted for expressi |
| 6329162 |
Biological fluid assay methods |
December 11, 2001 |
| A method to assess the level of folate in a biological sample comprises:providing said sample with glycine N-methyltransferase (GMT) and with an excess of S-adenosyl methionine (SAM) and of glycine;providing a control which contains no folate with said GMT and excess SAM and glycine in compa |
| 6261596 |
Method to provide for production of hair coloring pigments in hair follicles |
July 17, 2001 |
| The present invention describes a method for targeted and specific delivery of beneficial compounds, including hair dyes, melanin, proteins, and nucleic acids for gene therapy, to hair follicle cells using liposomes encapsulating the beneficial compound. Particularly preferred methods |
| 6251384 |
Metastasis models using green fluorescent protein (GFP) as a marker |
June 26, 2001 |
| A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is dis |
| 6235968 |
Metastasis models using green fluorescent protein (GFP) as a marker |
May 22, 2001 |
| A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is dis |
| 6235967 |
Metastasis models using green fluorescent protein as a marker |
May 22, 2001 |
| A method to follow the progression of metastasis of a primary tumor, which method comprises optionally removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the tissues in situ or observing the excised tissues |
| 6232523 |
Metastasis models using green fluorescent protein (GFP) as a marker |
May 15, 2001 |
| A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is dis |
| 6231854 |
Methioninase formulations and use in diagnostic methods |
May 15, 2001 |
| The present invention discloses the cloning of a methioninase-encoding nucleic acid molecule from Pseudomonas putida and the construction of high-level expression modules containing the methioninase-encoding nucleic acid molecule. The invention further provides expression modules that us |
| 6224901 |
Method for delivering beneficial compositions to hair follicles |
May 1, 2001 |
| The present invention is directed to introduce a replacement pigment into the hair shaft through the hair follicle using a formulation of a replacement pigment in a liposomal composition. |
| 6140102 |
High specificity homocysteinases and genes therefor |
October 31, 2000 |
| Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The n |
| 6080127 |
Skin vibration method for topical targeted delivery of beneficial agents into hair follicles |
June 27, 2000 |
| The present invention provides a methods and apparatuses for use in providing rapid, selective and effective targeted delivery of beneficial substances into hair follicles. The invention relies on the use of vibrational method of delivery of substances of different size from small mo |
| 6066467 |
High specificity homocysteine assays for biological samples |
May 23, 2000 |
| Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The n |
| 5998191 |
High specificity homocysteine assays for biological samples |
December 7, 1999 |
| Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The n |
| 5985540 |
High specificity homocysteine assays for biological samples |
November 16, 1999 |
| Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The n |
| 5965157 |
Method to provide for production of hair coloring pigments in hair follicles |
October 12, 1999 |
| The present invention provides a method to specifically target hair follicles with formulations which effect the production of hair coloring pigments in the follicle. Liposomal formulations for this purpose are disclosed. |
| 5914126 |
Methods to deliver macromolecules to hair follicles |
June 22, 1999 |
| The invention provides methods to deliver macromolecules to hair follicles selectively using formulations of these macromolecules in liposomal separations. |
| 5891704 |
Method to produce high levels of methioninase |
April 6, 1999 |
| The present invention discloses the cloning of a methioninase-encoding nucleic acid molecule from Pseudomonas putida and the construction of high-level expression modules containing the methioninase-encoding nucleic acid molecule. The invention further provides expression modules that us |
| 5888506 |
Methioninase formulations |
March 30, 1999 |
| Improved formulations of methioninase which contain methioninase in crystalline, lyophilized, or conjugated form are disclosed. The methioninase in these compositions has a specific activity of at least 20 U/mg and the formulations are detergent free and contain less than 1 ng endoto |
| 5849579 |
Native-state histoculturing method for skin |
December 15, 1998 |
| Methods and compositions for a native-state histoculturing system for skin are disclosed. Skin samples are placed on an extracellular support matrix immersed in a medium whereby the internal surface of the skin is adjacent to the matrix and the external surface is exposed above the surfa |
| 5830177 |
Skin vibration method for topical targeted delivery of beneficial agents into hair follicles |
November 3, 1998 |
| The present invention provides a methods and apparatuses for use in providing rapid, selective and effective targeted delivery of beneficial substances into hair follicles. The invention relies on the use of vibrational method of delivery of substances of different size from small mo |
| 5753263 |
Method to deliver compositions conferring resistance to alopecia to hair follicles |
May 19, 1998 |
| The invention describes a method to deliver a composition selectively to hair follicles using a liposomal formulation. Proteins which are cell cycle inhibitors are products of the multi-drug resistance gene or the recombinant materials for their production are targeted to hair follicles |
| 5726009 |
Native-state method and system for determining viability and proliferative capacity of tissues i |
March 10, 1998 |
| The present invention relates to a method of using an in vitro culture system to measure the cellular proliferative capacity cellular viability of human tissues, particularlytumor tissues. The invention also describes the use of the method to evaluate the effectiveness of an antineoplast |
| 5715835 |
Methods for treating and reducing the potential for cardiovascular disease using methioninase co |
February 10, 1998 |
| The present invention provides methods for treating and reducing the potential for cardiovascular disease using methioninase compositions having less than 10 ng endotoxin per mg methioninase. |
| 5690929 |
Use of methioninase and chemotherapy agents in chemotherapy |
November 25, 1997 |
| The present invention describes methods for using methioninase as an antitumor reagent in anti-methionine chemotherapy. Specifically, methioninase is used to slow or stop cell division which can be enhanced with competitive inhibitors of methionine. In addition, methioninase can be u |
| 5641508 |
Method for delivering melanin to hair follicles |
June 24, 1997 |
| The present invention describes a method for targeted and specific delivery of beneficial compounds, including dyes, proteins, and nucleic acids for gene therapy, to hair follicle cells using liposomes encapsulating the beneficial compound. Particularly preferred methods describe deliver |
| 5474909 |
Noncolorimetric histoculture method for predicting drug response of tumors |
December 12, 1995 |
| A method of evaluating the effectiveness of drugs in inhibiting the growth of tumor cells. A sample of a tumor is histocultured, a drug to be evaluated is added to the sample and the sample is incubated. A suitable tetrazolium salt is added and a frozen section of the sample is prepared. |
