| Patent Number |
Title Of Patent |
Date Issued |
| D463031 |
Microvolume sample plate |
September 17, 2002 |
|
| 7037654 |
Methods and compositions for enhancing detection in determinations employing cleavable electroph |
May 2, 2006 |
| Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or |
| 7001725 |
Kits employing generalized target-binding e-tag probes |
February 21, 2006 |
| Kits for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-t |
| 6964735 |
Method for moving charged particles |
November 15, 2005 |
| Devices and methods are disclosed for moving charged molecules through a medium by the application of a plurality of electrical fields of sufficient strength and applied for sufficient amounts of time so as to move the charged molecules through the medium. The devices although prefer |
| 6955874 |
Kits employing oligonucleotide-binding e-tag probes |
October 18, 2005 |
| Kits for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, capture agent and optionally a |
| 6949347 |
Multiplex analysis using membrane-bound sensitizers |
September 27, 2005 |
| The present invention is directed to methods and compositions for determining the presence, absence, and/or amounts of one or more membrane-associated analytes in a sample. In accordance with the invention, binding compounds derivatized with releasable molecular tags specifically bin |
| 6939451 |
Microfluidic chip having integrated electrodes |
September 6, 2005 |
| A microfluidic device having integrated components for conducting chemical operations. Depending upon the desired application, the components include electrodes for manipulating charged entities, heaters, electrochemical detectors, sensors for temperature, pH, fluid flow, and other usefu |
| 6916612 |
Sets of oligonucleotide-binding e-tag probes |
July 12, 2005 |
| Probe sets for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or "e-tag probes", comprising a detection |
| 6908594 |
Efficient microfluidic sealing |
June 21, 2005 |
| Improved sealing for microstructures in microfluidic devices having a plurality of units is provided by providing collars surrounding the openings to the microstructures, such as reservoirs. The collars are protrusions extending from the surface of the devices and the internal walls |
| 6906797 |
Side light activated microfluid channels |
June 14, 2005 |
| Devices are provided comprising reflecting channels for directing horizontally a vertical light beam through a multiplicity of streams. The devices provide particular applications in microfluidics, where streams of narrow cross-sectional dimensions are involved. Desirably, channels havin |
| 6905583 |
Closed-loop control of electrokinetic processes in microfluidic devices based on optical reading |
June 14, 2005 |
| A method for performing an electrophoretic separation on a microfluidic device includes applying an electric field to electrokinetically move a sample along a channel towards a location. The sample may be concentrated by application of the electric field. The method further comprises |
| 6900889 |
Submersible light-directing member for material excitation in microfluidic devices |
May 31, 2005 |
| A system for material excitation in microfluidic devices is described. Aspects of the invention resemble a submerged periscope when in use. They allow for light to be redirected along a more advantageous trajectory as does the maritime device. A prism with a reflecting surface may be use |
| 6846645 |
Multiplexed enzymatic assays |
January 25, 2005 |
| A mutiplexed enzyme assay method and substrate set for performing the method are disclosed. The method includes performing a plurality of enzyme reactions in the presence of a plurality of enzymes substrates, under conditions effective to convert an enzyme substrate to a corresponding |
| 6838680 |
Multiplexed fluorescent detection in microfluidic devices |
January 4, 2005 |
| An optical detection and orientation device is provided comprising a housing having an excitation light source, an optical element for reflecting the excitation light to an aspherical lens and transmitting light emitted by a fluorophore excited by said excitation light, a focusing le |
| 6838156 |
Method for linking two plastic work pieces without using foreign matter |
January 4, 2005 |
| The invention relates to a method for linking two adjacent plastic work pieces (2, 3). The inventive method is characterized in that the intended contact zone (K) of at least one of the two work pieces (2) with which the latter adjoins to the other work piece (3) is at least partially su |
| 6827906 |
Continuous form microstructure assay array |
December 7, 2004 |
| A continuous form microstructure array device is constructed as a flexible elongate film laminate containing a plurality of microstructures or arrays of microstructures arranged serially lengthwise along the laminate. The laminate can be continuously drawn from a supply roll or stack, ad |
| 6824663 |
Efficient compound distribution in microfluidic devices |
November 30, 2004 |
| Microfluidic devices are provided having units of 8-fold symmetry comprising 8 assay units, where a reservoir provides a common component to 8 assay units. The units can be compactly formed in a substrate to provide the ability to perform a large number of assays within a small area. The |
| 6818399 |
Methods employing generalized target-binding e-tag probes |
November 16, 2004 |
| Methods for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The methods include the use of electrophoretic tag prob |
| 6818113 |
Microfluidic device with sample injector and method of using |
November 16, 2004 |
| A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that |
| 6808609 |
Device and method for moving charged particles |
October 26, 2004 |
| Devices and methods are disclosed for moving charged molecules through a medium by the application of a plurality of electrical fields of sufficient strength and applied for sufficient amounts of time so as to move the charged molecules through the medium. The devices although prefer |
| 6803019 |
Laminate microstructure device and method for making same |
October 12, 2004 |
| A continuous form microstructure array device (20) is constructed as a flexible elongate film laminate containing microstructure arrays (26) arranged serially along the laminate. The laminate can be continuously drawn from a roll, passed through a processing and analysis device and r |
| 6787016 |
Dynamic coating with linear polymer mixture for electrophoresis |
September 7, 2004 |
| Compositions and methods are provided for performing capillary electrophoresis using a composition comprising in combination in an aqueous buffered medium a coating polymer and a sieving polymer, where the sieving polymer is more hydrophilic than the coating polymer and is present in |
| 6787015 |
Methods for conducting electrophoretic analysis |
September 7, 2004 |
| Capillary electrophoresis is performed under conventional conditions in microchannels having a norbornene based polymer surface. The norbornene based polymers can be used as a solid substrate for forming the necessary features for a microfluidic device, where the entire device may be mad |
| 6730206 |
Microfluidic device and system with improved sample handling |
May 4, 2004 |
| An improved microfluidics device and system for sample loading and injection are disclosed. The device includes three main channels--a separation channel, supply channel, and drain channel--for use in loading and injecting a sample from the supply channel. Pairs of peripheral channel |
| 6686152 |
Methods employing oligonucleotide-binding e-tag probes |
February 3, 2004 |
| Methods for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The methods include the use of electrophoretic tag probes or e-tag probes, comprising a detection |
| 6685813 |
Tandem isotachophoresis/zone electrophoresis method and system |
February 3, 2004 |
| A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable |
| 6682887 |
Detection using degradation of a tagged sequence |
January 27, 2004 |
| Methods and compositions are provided for detecting DNA sequences, particularly single nucleotide polymorphisms, using a pair of nucleotide sequences, a primer and a snp detection sequence, where the snp detection sequence binds downstream from the primer to the target DNA in the dir |
| 6673550 |
Electrophoretic tag reagents comprising fluorescent compounds |
January 6, 2004 |
| Electrophoretic probes comprising fluorescent compounds as detection groups and mobility modifiers are disclosed for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. In one embodiment, detection involves the |
| 6649351 |
Methods for detecting a plurality of analytes by mass spectrometry |
November 18, 2003 |
| The invention provides a method for detecting a target analyte, by: (a) contacting one or more target analytes with a set of first and second binding reagents under conditions sufficient for binding of a target analyte with the first and second binding reagents, each of the first bin |
| 6632630 |
Monooxygenase assays |
October 14, 2003 |
| Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and |
| 6632606 |
Methods for single nucleotide polymorphism detection |
October 14, 2003 |
| Methods and compositions are provided for determining large numbers of single nucleotide polymorphisms in target DNA employing particles having (1) primers complementary to sequences in the target DNA where the next succeeding 3'-nucleotide is a potential single nucleotide polymorphism a |
| 6630296 |
Multiplexed enzymatic assays |
October 7, 2003 |
| A multiplexed enzyme assay method and substrate set for performing the method are disclosed. The method includes performing a plurality of enzyme reactions in the presence of a plurality of enzymes substrates, under conditions effective to convert an enzyme substrate to a corresponding |
| 6627406 |
Sample evaporative control |
September 30, 2003 |
| Devices and methods are provided using microfluidic devices for manipulating small volumes and determining a variety of chemical and physical events. The devices rely upon an opening to the atmosphere of a small volume in a zone, where a sample is placed in the zone where evaporation |
| 6627400 |
Multiplexed measurement of membrane protein populations |
September 30, 2003 |
| Families of compositions are provided as labels, referred to as eTag reporters for attaching to polymeric compounds and assaying based on release of the eTag reporters from the polymeric compound and separation and detection. For oligonucleotides, the eTag reporters are synthesized at |
| 6623860 |
Multilevel flow structures |
September 23, 2003 |
| Multilevel microfluidic structures and their use are provided for performing operations using electrokinetic or pneumatic force for moving sample components through and between levels. The devices have flow systems comprising microstructures including reservoirs, channels and vias, w |
| 6614030 |
Multiplexed fluorescent detection in microfluidic devices |
September 2, 2003 |
| An optical detection and orientation device is provided comprising housing having an excitation light source, an optical element for reflecting the excitation light to an aspherical lens and transmitting light emitted by a fluorophore excited by said excitation light, a focussing lens fo |
| 6613525 |
Microfluidic apparatus and method for purification and processing |
September 2, 2003 |
| Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said ma |
| 6613211 |
Capillary electrokinesis based cellular assays |
September 2, 2003 |
| Cell based assays are performed in a microfluidic device, where the cells are introduced into a reservoir and are contacted with one or more agents prior to or during their residence in the reservoir or in a capillary channel connected to the reservoir. The cells are moved by electrokine |
| 6558945 |
Method and device for rapid color detection |
May 6, 2003 |
| Methods and apparatus are provided for miniaturized DNA sequencing using four-color detection. The method employs a multichannel chip where the channels are simultaneously irradiated to excite fluorophores in the channels. The emitted light is divided into four different wavelength b |
| 6555389 |
Sample evaporative control |
April 29, 2003 |
| Devices and methods are provided using microfluidic devices for manipulating small volumes and determining a variety of chemical and physical events. The devices rely upon an opening to the atmosphere of a small volume in a zone, where a sample is placed in the zone where evaporation |
| 6515753 |
Optical alignment in capillary detection using capillary wall scatter |
February 4, 2003 |
| The present invention provides for methods and apparatus for locating a capillary channel that is disposed within a lab chip. The method provides for scanning the channel with a light source, monitoring the resulting light at the edges of the lab chip, and interpreting this information |
| 6514700 |
Nucleic acid detection using degradation of a tagged sequence |
February 4, 2003 |
| Methods and compositions are provided for detecting target molecules, e.g. DNA sequences, particularly single nucleotide polymorphisms, using a pair of nucleotide sequences, a primer and a snp detection sequence, where the snp detection sequence binds downstream from the primer to the ta |
| 6399952 |
Multiplexed fluorescent detection in microfluidic devices |
June 4, 2002 |
| An optical detection and orientation device is provided comprising housing having an excitation light source, an optical element for reflecting the excitation light to an aspherical lens and transmitting light emitted by a fluorophore excited by said excitation light, a focussing lens fo |
| 6344326 |
Microfluidic method for nucleic acid purification and processing |
February 5, 2002 |
| Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said ma |
| 6322980 |
Single nucleotide detection using degradation of a fluorescent sequence |
November 27, 2001 |
| Methods and compositions are provided for detecting single nucleotide polymorphisms using a pair of oligonucleotides, a primer and a snp detection sequence, where the snp detection sequence hybridizes to the target DNA downstream from the primer and in the direction of primer extensi |
| 6306273 |
Methods and compositions for conducting processes in microfluidic devices |
October 23, 2001 |
| A method is disclosed for controlling the direction and transport of a material on a microfluidic device formed from an acrylic polymer, by electrokinetic flow of a fluid containing the material. The method comprises providing an electrokinesis buffer containing a charged hydrophilic |
| 6284113 |
Apparatus and method for transferring liquids |
September 4, 2001 |
| The present invention concerns devices, apparatus and methods for transferring liquids. One aspect of the present invention is a device comprising a plate having a plurality of transfer elements. Each of the transfer elements comprises an aperture in the plate where the aperture is c |
| 6176962 |
Methods for fabricating enclosed microchannel structures |
January 23, 2001 |
| Methods are provided for the fabrication of polymeric microchannel structures having enclosed microchannels of capillary dimension. The microchannel structures are constructed of a base plate and a cover, sealed together. Microchannel structures having walls of a plastic material are |
| 6103537 |
Capillary assays involving separation of free and bound species |
August 15, 2000 |
| The present invention concerns methods for masking inhomogeneity of a member of a specific binding pair (sbp) employed in a capillary electroseparation. The method comprises binding the sbp member to synthetic particles that become localized during capillary electroseparation. Also d |
| 6103199 |
Capillary electroflow apparatus and method |
August 15, 2000 |
| The present invention concerns an apparatus for conducting a microfluidic process. The apparatus comprises integral first and second plates. The first plate comprises an array of sample receiving elements for receiving a plurality of samples from an array of sample containers and dispens |
